Persistent TFIIH binding to non-excised DNA damage causes cell and developmental failure.
Nat Commun
; 15(1): 3490, 2024 Apr 25.
Article
en En
| MEDLINE
| ID: mdl-38664429
ABSTRACT
Congenital nucleotide excision repair (NER) deficiency gives rise to several cancer-prone and/or progeroid disorders. It is not understood how defects in the same DNA repair pathway cause different disease features and severity. Here, we show that the absence of functional ERCC1-XPF or XPG endonucleases leads to stable and prolonged binding of the transcription/DNA repair factor TFIIH to DNA damage, which correlates with disease severity and induces senescence features in human cells. In vivo, in C. elegans, this prolonged TFIIH binding to non-excised DNA damage causes developmental arrest and neuronal dysfunction, in a manner dependent on transcription-coupled NER. NER factors XPA and TTDA both promote stable TFIIH DNA binding and their depletion therefore suppresses these severe phenotypical consequences. These results identify stalled NER intermediates as pathogenic to cell functionality and organismal development, which can in part explain why mutations in XPF or XPG cause different disease features than mutations in XPA or TTDA.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Daño del ADN
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Caenorhabditis elegans
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Proteínas de Unión al ADN
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Reparación del ADN
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Endonucleasas
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Factor de Transcripción TFIIH
Límite:
Animals
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Humans
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
/
CIENCIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Países Bajos