Amphiphilic Rhodamine Fluorescent Probes Combined with Basal Imaging for Fine Structures of the Cell Membrane.
Anal Chem
; 96(18): 7257-7264, 2024 05 07.
Article
en En
| MEDLINE
| ID: mdl-38664861
ABSTRACT
Confocal fluorescence imaging of fine structures of the cell membrane is important for understanding their biofunctions but is often neglected due to the lack of an effective method. Herein, we develop new amphiphilic rhodamine fluorescent probe RMGs in combination with basal imaging for this purpose. The probes show high signal-to-noise ratio and brightness and low internalization rate, making them suitable for imaging the fine substructures of the cell membrane. Using the representative probe RMG3, we not only observed the cell pseudopodia and intercellular nanotubes but also monitored the formation of migrasomes in real time. More importantly, in-depth imaging studies on more cell lines revealed for the first time that hepatocellular carcinoma cells secreted much more adherent extracellular vesicles than other cell lines, which might serve as a potential indicator of liver cells. We believe that RMGs may be useful for investigating the fine structures of the cell membrane.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Rodaminas
/
Membrana Celular
/
Colorantes Fluorescentes
Límite:
Humans
Idioma:
En
Revista:
Anal Chem
/
Anal. chem
/
Analytical chemistry
Año:
2024
Tipo del documento:
Article
País de afiliación:
China