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Dysfunction of Gpl1-Gih35-Wdr83 Complex in S. pombe Affects the Splicing of DNA Damage Repair Factors Resulting in Increased Sensitivity to DNA Damage.
Cipakova, Ingrid; Jurcik, Matus; Selicky, Tomas; Lalakova, Laura Olivia; Jakubikova, Jana; Cipak, Lubos.
Afiliación
  • Cipakova I; Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
  • Jurcik M; Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
  • Selicky T; Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
  • Lalakova LO; Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
  • Jakubikova J; Department of Tumor Immunology, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
  • Cipak L; Department of Genetics, Cancer Research Institute, Biomedical Research Center, Slovak Academy of Sciences, Dubravska cesta 9, 84505 Bratislava, Slovakia.
Int J Mol Sci ; 25(8)2024 Apr 10.
Article en En | MEDLINE | ID: mdl-38673778
ABSTRACT
Pre-mRNA splicing plays a key role in the regulation of gene expression. Recent discoveries suggest that defects in pre-mRNA splicing, resulting from the dysfunction of certain splicing factors, can impact the expression of genes crucial for genome surveillance mechanisms, including those involved in cellular response to DNA damage. In this study, we analyzed how cells with a non-functional spliceosome-associated Gpl1-Gih35-Wdr83 complex respond to DNA damage. Additionally, we investigated the role of this complex in regulating the splicing of factors involved in DNA damage repair. Our findings reveal that the deletion of any component within the Gpl1-Gih35-Wdr83 complex leads to a significant accumulation of unspliced pre-mRNAs of DNA repair factors. Consequently, mutant cells lacking this complex exhibit increased sensitivity to DNA-damaging agents. These results highlight the importance of the Gpl1-Gih35-Wdr83 complex in regulating the expression of DNA repair factors, thereby protecting the stability of the genome following DNA damage.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño del ADN / Empalme del ARN / Reparación del ADN / Factores de Empalme de ARN Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño del ADN / Empalme del ARN / Reparación del ADN / Factores de Empalme de ARN Idioma: En Revista: Int J Mol Sci Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia