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Targeting c-Myc with decoy oligodeoxynucleotide-loaded polycationic nanoparticles inhibits cell growth and induces apoptosis in cancer stem-like cells (NTERA-2).
Ghorbani, Roghayeh; Gharbavi, Mahmoud; Keshavarz, Benyamin; Madanchi, Hamid; Johari, Behrooz.
Afiliación
  • Ghorbani R; Department of Medical Biotechnology, School of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
  • Gharbavi M; Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran.
  • Keshavarz B; Nanotechnology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Madanchi H; Pain Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
  • Johari B; Student Research Committee, Zanjan University of Medical Sciences, Zanjan, Iran.
Mol Biol Rep ; 51(1): 623, 2024 May 06.
Article en En | MEDLINE | ID: mdl-38710891
ABSTRACT

BACKGROUND:

An increase in cancer stem cell (CSC) populations and their resistance to common treatments could be a result of c-Myc dysregulations in certain cancer cells. In the current study, we investigated anticancer effects of c-Myc decoy ODNs loaded-poly (methacrylic acid-co-diallyl dimethyl ammonium chloride) (PMA-DDA)-coated silica nanoparticles as carriers on cancer-like stem cells (NTERA-2). METHODS AND

RESULTS:

The physicochemical characteristics of the synthesized nanocomposites (SiO2@PMA-DDA-DEC) were analyzed using FT-IR, DLS, and SEM techniques. UV-Vis spectrophotometer was applied to analyze the release pattern of decoy ODNs from the nanocomposite. Furthermore, uptake, cell viability, apoptosis, and cell cycle assays were used to investigate the anticancer effects of nanocomposites loaded with c-Myc decoy ODNs on NTERA-2 cancer cells. The results of physicochemical analytics demonstrated that SiO2@PMA-DDA-DEC nanocomposites were successfully synthesized. The prepared nanocomposites were taken up by NTERA-2 cells with high efficiency, and could effectively inhibit cell growth and increase apoptosis rate in the treated cells compared to the control group. Moreover, SiO2@PMA-DDA nanocomposites loaded with c-Myc decoy ODNs induced cell cycle arrest at the G0/G1 phase in the treated cells.

CONCLUSIONS:

The conclusion drawn from this study is that c-Myc decoy ODN-loaded SiO2@PMA-DDA nanocomposites can effectively inhibit cell growth and induce apoptosis in NTERA-2 cancer cells. Moreover, given that a metal core is incorporated into this synthetic nanocomposite, it could potentially be used in conjunction with irradiation as part of a decoy-radiotherapy combinational therapy in future investigations.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Proteínas Proto-Oncogénicas c-myc / Apoptosis / Proliferación Celular / Nanopartículas Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Madre Neoplásicas / Proteínas Proto-Oncogénicas c-myc / Apoptosis / Proliferación Celular / Nanopartículas Límite: Humans Idioma: En Revista: Mol Biol Rep Año: 2024 Tipo del documento: Article País de afiliación: Irán