The single-cell chromatin landscape in gonadal cell lineage specification.
BMC Genomics
; 25(1): 464, 2024 May 13.
Article
en En
| MEDLINE
| ID: mdl-38741085
ABSTRACT
Gonad development includes sex determination and divergent maturation of the testes and ovaries. Recent advances in measuring gene expression in single cells are providing new insights into this complex process. However, the underlying epigenetic regulatory mechanisms remain unclear. Here, we profiled chromatin accessibility in mouse gonadal cells of both sexes from embryonic day 11.5 to 14.5 using single-cell assay for transposase accessible chromatin by sequencing (scATAC-seq). Our results showed that individual cell types can be inferred by the chromatin landscape, and that cells can be temporally ordered along developmental trajectories. Integrative analysis of transcriptomic and chromatin-accessibility maps identified multiple putative regulatory elements proximal to key gonadal genes Nr5a1, Sox9 and Wt1. We also uncover cell type-specific regulatory factors underlying cell type specification. Overall, our results provide a better understanding of the epigenetic landscape associated with the progressive restriction of cell fates in the gonad.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Cromatina
/
Linaje de la Célula
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Factor de Transcripción SOX9
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Análisis de la Célula Individual
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Gónadas
Límite:
Animals
Idioma:
En
Revista:
BMC Genomics
Asunto de la revista:
GENETICA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China