A probe for NIR-II imaging and multimodal analysis of early Alzheimer's disease by targeting CTGF.
Nat Commun
; 15(1): 5000, 2024 Jun 12.
Article
en En
| MEDLINE
| ID: mdl-38866763
ABSTRACT
To date, earlier diagnosis of Alzheimer's disease (AD) is still challenging. Recent studies revealed the elevated expression of connective tissue growth factor (CTGF) in AD brain is an upstream regulator of amyloid-beta (Aß) plaque, thus CTGF could be an earlier diagnostic biomarker of AD than Aß plaque. Herein, we develop a peptide-coated gold nanocluster that specifically targets CTGF with high affinity (KD ~ 21.9 nM). The probe can well penetrate the blood-brain-barrier (BBB) of APP/PS1 transgenic mice at early-stage (earlier than 3-month-old) in vivo, allowing non-invasive NIR-II imaging of CTGF when there is no appearance of Aß plaque deposition. Notably, this probe can also be applied to measuring CTGF on postmortem brain sections by multimodal analysis, including fluorescence imaging, peroxidase-like chromogenic imaging, and ICP-MS quantitation, which enables distinguishment between the brains of AD patients and healthy people. This probe possesses great potential for precise diagnosis of earlier AD before Aß plaque formation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Encéfalo
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Ratones Transgénicos
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Factor de Crecimiento del Tejido Conjuntivo
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Enfermedad de Alzheimer
Límite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Nat Commun
Asunto de la revista:
BIOLOGIA
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CIENCIA
Año:
2024
Tipo del documento:
Article