LW-1 induced resistance to TMV in tobacco was mediated by nitric oxide and salicylic acid pathway.
Pestic Biochem Physiol
; 202: 105896, 2024 Jun.
Article
en En
| MEDLINE
| ID: mdl-38879345
ABSTRACT
The objective of this study was to investigate the mechanism underlying LW-1-induced resistance to TMV in wild-type and salicylic acid (SA)-deficient NahG transgenic tobacco plants. Our findings revealed that LW-1 failed to induce antivirus infection activity and increase SA content in NahG tobacco, indicating the crucial role of SA in these processes. Meanwhile, LW-1 triggered defense-related early-signaling nitric oxide (NO) generation, as evidenced by the emergence of NO fluorescence in both types of tobacco upon treatment with LW-1, however, NO fluorescence was stronger in NahG compared to wild-type tobacco. Notably, both of them were eliminated by the NO scavenger cPTIO, which also reversed LW-1-induced antivirus activity and the increase of SA content, suggesting that NO participates in LW-1-induced resistance to TMV, and may act upstream of the SA pathway. Defense-related enzymes and genes were detected in tobacco with or without TMV inoculation, and the results showed that LW-1 regulated both enzyme activity (ß-1,3-glucanase [GLU], catalase [CAT] and phenylalanine ammonia-lyase [PAL]) and gene expression (PR1, PAL, WYKY4) through NO signaling in both SA-dependent and SA-independent pathways.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Plantas
/
Nicotiana
/
Virus del Mosaico del Tabaco
/
Ácido Salicílico
/
Resistencia a la Enfermedad
/
Óxido Nítrico
Idioma:
En
Revista:
Pestic Biochem Physiol
Año:
2024
Tipo del documento:
Article
País de afiliación:
China