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Transcription templated assembly of the nucleolus in the C. elegans embryo.
Kodan, Nishant; Hussaini, Rabeya; Weber, Stephanie C; Kondev, Jane; Mohapatra, Lishibanya.
Afiliación
  • Kodan N; School of Physics and Astronomy, College of Science, Rochester Institute of Technology, Rochester, NY 14623, USA.
  • Hussaini R; Department of Physics, New York University, New York, NY 10003, USA.
  • Weber SC; Department of Biology, McGill University, Montreal, QC H3A 1B1, Canada.
  • Kondev J; Department of Physics, McGill University, Montreal, QC H3A 2T8, Canada.
  • Mohapatra L; Department of Physics, Brandeis University, Waltham, MA 02454, USA.
bioRxiv ; 2024 Jun 06.
Article en En | MEDLINE | ID: mdl-38895351
ABSTRACT
The nucleolus is a multicomponent structure made of RNA and proteins that serves as the site of ribosome biogenesis within the nucleus. It has been extensively studied as a prototype of a biomolecular condensate whose assembly is driven by phase separation. While the steady-state size of the nucleolus is quantitatively accounted for by the thermodynamics of phase separation, we show that experimental measurements of the assembly dynamics are inconsistent with a simple model of a phase-separating system relaxing to its equilibrium state. Instead, we show that the dynamics are well described by a model in which the transcription of ribosomal RNA actively drives nucleolar assembly. We find that our model of active transcription-templated assembly quantitatively accounts for the rapid kinetics observed in early embryos at different developmental stages, and for different RNAi perturbations of embryo size. Our model predicts a scaling of the time to assembly with the volume of the nucleus to the one-third power, which is confirmed by experimental data. Our study highlights the role of active processes such as transcription in controlling the placement and timing of assembly of membraneless organelles.
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Texto completo: 1 Banco de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Banco de datos: MEDLINE Idioma: En Revista: BioRxiv Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos