Repeated platelet-rich plasma injections improve erectile dysfunction in a rat model of hyperhomocysteinemia.
Asian J Androl
; 2024 Jul 02.
Article
en En
| MEDLINE
| ID: mdl-38953713
ABSTRACT
ABSTRACT Platelet-rich plasma (PRP) shows promise as a regenerative modality for mild-to-moderate erectile dysfunction (ED). However, its efficacy in treating severe ED remains unknown. Blood samples from 8-week-old male rats were used to prepare PRP through a two-step centrifugation procedure, followed by chitosan activation and freezeâthaw cycle. A hyperhomocysteinemia (HHcy)-related ED model was established using a methionine-enriched diet, and an apomorphine (APO) test was conducted during the 4 th week. APO-negative rats were divided into two groups and were injected with PRP or saline every 2 weeks. Erectile function and histological analyses of the corpus cavernosum were performed during the 16 th week. The results revealed that erectile function was significantly impaired in rats with HHcy-related ED compared to that in age-matched rats but was improved by repeated PRP injections. Immunofluorescence staining revealed a reduction in reactive oxygen species and additional benefits on the recovery of structures within the corpus cavernosum in rats that received PRP treatment compared to those in the saline-injected control group. Therefore, PRP could enhance functional and structural recovery in a severe HHcy-related ED model. A notable strength of the present study lies in the use of a repeated intracavernous injection method, mirroring protocols used in human studies, which offers more reliable results for translating the findings to humans.
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Banco de datos:
MEDLINE
Idioma:
En
Revista:
Asian J Androl
Asunto de la revista:
MEDICINA REPRODUTIVA
/
UROLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
China