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Serial X-ray liquidography: multi-dimensional assay framework for exploring biomolecular structural dynamics with microgram quantities.
Kim, Seong Ok; Yun, So Ri; Lee, Hyosub; Jo, Junbeom; Ahn, Doo-Sik; Kim, Doyeong; Kosheleva, Irina; Henning, Robert; Kim, Jungmin; Kim, Changin; You, Seyoung; Kim, Hanui; Lee, Sang Jin; Ihee, Hyotcherl.
Afiliación
  • Kim SO; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Yun SR; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • Lee H; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Jo J; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • Ahn DS; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Kim D; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • Kosheleva I; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Henning R; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • Kim J; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Kim C; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • You S; Center for Advanced Reactions Dynamics (CARD), Institute for Basic Science (IBS), Daejeon, 34141, Republic of Korea.
  • Kim H; Department of Chemistry, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, 34141, Republic of Korea.
  • Lee SJ; Center for Advanced Radiation Sources, The University of Chicago, 9700 South Cass Avenue, Argonne, IL, 60439, USA.
  • Ihee H; Center for Advanced Radiation Sources, The University of Chicago, 9700 South Cass Avenue, Argonne, IL, 60439, USA.
Nat Commun ; 15(1): 6287, 2024 Jul 26.
Article en En | MEDLINE | ID: mdl-39060271
ABSTRACT
Understanding protein structure and kinetics under physiological conditions is crucial for elucidating complex biological processes. While time-resolved (TR) techniques have advanced to track molecular actions, their practical application in biological reactions is often confined to reversible photoreactions within limited experimental parameters due to inefficient sample utilization and inflexibility of experimental setups. Here, we introduce serial X-ray liquidography (SXL), a technique that combines time-resolved X-ray liquidography with a fixed target of serially arranged microchambers. SXL breaks through the previously mentioned barriers, enabling microgram-scale TR studies of both irreversible and reversible reactions of even a non-photoactive protein. We demonstrate its versatility in studying a wide range of biological reactions, highlighting its potential as a flexible and multi-dimensional assay framework for kinetic and structural characterization. Leveraging X-ray free-electron lasers and micro-focused X-ray pulses promises further enhancements in both temporal resolution and minimizing sample quantity. SXL offers unprecedented insights into the structural and kinetic landscapes of molecular actions, paving the way for a deeper understanding of complex biological processes.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article