Protocol for differential analysis of Trim71-associated protein complexes in mouse embryonic stem cells by mass spectrometry using Perseus.

ABSTRACT

Characterizing multi-protein complexes using mass spectrometry is crucial for understanding complex biochemical activities that govern cell fate. Investigating dynamic protein-protein interactions requires diverse qualitative and quantitative approaches. We present a protocol for differential analysis of Trim71-associated proteins in mouse embryonic stem cells under two conditions. We describe steps for cytoplasmic extraction, protein immunoprecipitation, sample preparation for mass spectrometry, and data analysis with Perseus. Our versatile tool enables in-depth exploration of protein complex compositional changes, contributing to a deeper understanding of cellular dynamics and making it suitable for various research domains. For complete details on the use and execution of this protocol, please refer to Rapone et al.1.