VitelloTag: a tool for high-throughput cargo delivery into oocytes.
Development
; 151(20)2024 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-39171380
ABSTRACT
Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Vitelogeninas
Límite:
Animals
Idioma:
En
Revista:
Development
/
Development (Camb.)
/
Development (Cambridge)
Asunto de la revista:
BIOLOGIA
/
EMBRIOLOGIA
Año:
2024
Tipo del documento:
Article
País de afiliación:
Estados Unidos