Specific assay for pregnancy-specific-beta 1-glycoprotein (Sp1-beta). Preparation of antiserum specific for SP1-beta by absorption with a crossreacting high molecular weight serum protein.
J Reprod Immunol
; 1(3): 159-66, 1979 Oct.
Article
en En
| MEDLINE
| ID: mdl-551173
ABSTRACT
Two pregnancy-associated proteins reacting with antibody against pregnancy-specific beta 1-glycoprotein (PS beta G or SP1) were separated by means of ammonium sulphate precipitation, size chromatography and preparative zone electrophoresis. The antibody preparation was made monospecific to the beta-mobile protein by liquid phase cross-absorption of anti-SP1 immunoglobulin preparation with the isolated alpha2-mobile high molecular weight protein. The specificity of the absorbed antibody was tested in line immunoelectrophoresis, rocket immunoelectrophoresis and crossed immunoelectrophoresis.
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Banco de datos:
MEDLINE
Asunto principal:
Glicoproteínas
/
Reacciones Cruzadas
/
Sueros Inmunes
/
Especificidad de Anticuerpos
Límite:
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
J Reprod Immunol
Año:
1979
Tipo del documento:
Article