N-Acylation of ethanolamine phospholipids in canine myocardium.
Biochim Biophys Acta
; 712(2): 342-55, 1982 Aug 18.
Article
en En
| MEDLINE
| ID: mdl-7126608
ABSTRACT
N-Acylethanolamine phospholipids, which are found in infarcted but not in normal canine myocardium, were produced by preparations of normal myocardial tissue incubated in the presence of millimolar concentrations of Ca2+. Biosynthetic activity from endogenous substrates was associated with both microsomal and mitochondrial fractions and exhibited an alkaline pH optimum. The time course of N-acylethanolamine phospholipid synthesis and degradation was followed after pulse labeling of myocardial ethanolamine phospholipids with [1,2-14C]ethanolamine. Enzymic N-acylation of both phosphatidylethanolamine and lysophosphatidylethanolamine was demonstrated by incubating these substrates with homogenates of myocardial tissue. Neither free fatty acids nor acyl-CoA derivatives served as acyl donor but some of the constituent fatty acids of exogenous phosphatidylethanolamine were recovered among the amide-linked fatty acids of the N-acylethanolamine phospholipids. N-Acylation may thus occur by the transfer of O-acyl groups catalyzed by a Ca2+ -dependent transacylase. Since N-acylethanolamine phospholipids are precursors of the biologically active N-acylethanolamines, their biosynthesis may constitute an important injury-induced metabolic event aimed at the protection of ischemic myocardial tissue.
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Banco de datos:
MEDLINE
Asunto principal:
Fosfolípidos
/
Etanolaminas
/
Miocardio
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1982
Tipo del documento:
Article