Sequences responsible for transcription termination of the mouse gastrin gene.
Biochem Mol Biol Int
; 35(6): 1205-13, 1995 May.
Article
en En
| MEDLINE
| ID: mdl-7492958
ABSTRACT
To identify the transcription termination elements in the mouse gastrin gene, we examined RNA transcripts after stable transfection of gastrin expression plasmids into the NIH3T3 cell line. The GT-repeat region at the 3'-flanking sequence of the mouse gastrin gene acted as a transcription terminator. When the GT-repeat unit was deleted from its site, the effect of termination disappeared. Further experiment, using serial deletion mutants, revealed that the 56-38 nucleotide upstream region from the GT-repeat unit also participated in transcription termination. We propose that the upstream region of the GT-repeat unit might be recognized as a pause site by the RNA polymerase II, and an abnormal DNA structure, derived from the GT-repeat unit, might function as a blockage of polymerase processivity.
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Banco de datos:
MEDLINE
Asunto principal:
Terminación de la Cadena Péptídica Traduccional
/
ARN Polimerasa II
/
Gastrinas
Límite:
Animals
Idioma:
En
Revista:
Biochem Mol Biol Int
Asunto de la revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Año:
1995
Tipo del documento:
Article