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Three-dimensional crystal structure of the A-tract DNA dodecamer d(CGCAAATTTGCG) complexed with the minor-groove-binding drug Hoechst 33258.
Vega, M C; García Sáez, I; Aymamí, J; Eritja, R; Van der Marel, G A; Van Boom, J H; Rich, A; Coll, M.
Afiliación
  • Vega MC; Departament de Biologia Molecular i Cel-lular, Centre d'Investigació i Desenvolupament-C.S.I.C., Barcelona, Spain.
Eur J Biochem ; 222(3): 721-6, 1994 Jun 15.
Article en En | MEDLINE | ID: mdl-7517864
The molecular structure of the DNA A-tract dodecamer d(CGCAAATTTGCG) complexed with the drug Hoechst 33258 has been determined by X-ray diffraction analysis. The Hoechst molecule binds in the DNA minor groove covering the sequence AATTT of the central A-tract, with the piperazine group close to one of the GC regions. The drug molecule makes two three-centered hydrogen bonds from the nitrogen atoms of the benzimidazole rings to the N3 and O2 atoms of the DNA bases. Although a high propeller twist is observed in the A-tract, only one unsymmetrical three-centered hydrogen bond is present in the DNA major groove. The structure is compared with other minor-groove-binding drug complexes and the influence of these drugs on DNA A-tracts is discussed.
Asunto(s)
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Banco de datos: MEDLINE Asunto principal: Oligodesoxirribonucleótidos / ADN / Bisbenzimidazol / Conformación de Ácido Nucleico Idioma: En Revista: Eur J Biochem Año: 1994 Tipo del documento: Article País de afiliación: España
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Banco de datos: MEDLINE Asunto principal: Oligodesoxirribonucleótidos / ADN / Bisbenzimidazol / Conformación de Ácido Nucleico Idioma: En Revista: Eur J Biochem Año: 1994 Tipo del documento: Article País de afiliación: España