[A reversed-phase HPLC method for determining tretinoin].
Zhongguo Yao Li Xue Bao
; 15(5): 458-61, 1994 Sep.
Article
en Zh
| MEDLINE
| ID: mdl-7717076
ABSTRACT
Tretinoin (Tre) and its active stereo isomer isotretinoin (Iso) were simultaneously determined by reversed-phase high pressure liquid chromatographic method with a uv detector adjusted to 348 nm. Separation was accomplished on YWG-C18 column by using a MeOHNH4Ac buffer (pH 6.0) 8515 (volvol), chlorpromazine (Chl) being chosen as internal standard. Minimal detectable amount of Tre was 0.5 ng. Calibration curve was linear (r = 0.9999) in the concentration range of 25-2500 ng.ml-1. This method was used to determinate the transdermal amounts of Tre from three different preparations in Franz diffusion cell in vitro. The results showed that the proposed method could distinguish the transdermal differences from various formulations or different skin samples. In addition, it is able to be used in quantitative analysis of Tre and Iso.
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Banco de datos:
MEDLINE
Asunto principal:
Tretinoina
/
Isotretinoína
Límite:
Animals
Idioma:
Zh
Revista:
Zhongguo Yao Li Xue Bao
Año:
1994
Tipo del documento:
Article
País de afiliación:
China