Human apolipoprotein A-IV gene expression is modulated by members of the nuclear hormone receptor superfamily.
Biochim Biophys Acta
; 1210(1): 41-7, 1993 Dec 02.
Article
en En
| MEDLINE
| ID: mdl-8257717
ABSTRACT
Human apolipoprotein A-IV (apo A-IV) is essentially synthesized in intestine and to a lower extent in liver. The presence of detectable amounts of apo A-IV messenger in HepG2 and Caco-2 cells enables us to study the transcription regulation of the gene in these cells. A main hypersensitive site to DNase I is detected in the DNA of HepG2 nuclei, in a region situated about 3400 base pairs upstream from the cap site. Transient expression experiments in HepG2, Caco-2 and HeLa cells indicate that HNF-4 activates the transcription of a reporter gene through the -3500/+67 sequence of the apo A-IV gene. This activation is repressed by another transcription factor, Ear3/COUP-TF. On the contrary, HNF-1, whose expression is controlled by HNF-4, is not involved in the regulation of the reporter gene transcription through the -6700/+67 genome sequence. These results indicate that the apo A-IV gene expression is modulated by orphan ligand members of the superfamily of nuclear hormone receptors.
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Banco de datos:
MEDLINE
Asunto principal:
Apolipoproteínas A
/
Fosfoproteínas
/
Factores de Transcripción
/
Regulación de la Expresión Génica
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1993
Tipo del documento:
Article
País de afiliación:
Francia