Improved assay for monocyte chemotaxis using frozen stored responder cells.
J Immunol Methods
; 14(1): 65-72, 1977.
Article
en En
| MEDLINE
| ID: mdl-833430
ABSTRACT
Frozen stored human mononuclear cells were compared with non-frozen cells as responder cells in the monocyte chemotaxis assay. Frozen stored cells were found to inhibit lower background migration, resulting in greater experimental-to-control ratios. The use of frozen cells also greatly improved the speed, standardization of conditions and day-to-day reproducibility of the assay procedure. Frozen cells were at least as sensitive as fresh cells for detecting low chemotactic factor concentrations and were superior for detecting differences in concentration.
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Banco de datos:
MEDLINE
Asunto principal:
Conservación de la Sangre
/
Monocitos
/
Quimiotaxis de Leucocito
Límite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Año:
1977
Tipo del documento:
Article