Lipospermine-mediated gene transfer technique into murine cultured cortical cells.
J Neurosci Methods
; 71(2): 183-6, 1997 Feb.
Article
en En
| MEDLINE
| ID: mdl-9128154
ABSTRACT
In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment. The procedure was optimized after testing several parameters DPPES/DNA ratio, incubation time, kinetics of transgene expression, and growth medium. The protocol was achieved by following the expression of the E. coli LacZ reporter gene under the control of the cytomegalovirus promoter. The lipopolyamine-mediated transfection is efficient for terminally differentiated cells, since we routinely obtained transfection efficiencies of 30% for neurons.
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Banco de datos:
MEDLINE
Asunto principal:
Plásmidos
/
Transfección
/
Espermina
/
Corteza Cerebral
/
Glicina
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
J Neurosci Methods
Año:
1997
Tipo del documento:
Article
País de afiliación:
Francia