Serologic reactivity using conserved envelope epitopes in feline lentivirus-infected felids.
J Vet Diagn Invest
; 9(2): 125-9, 1997 Apr.
Article
en En
| MEDLINE
| ID: mdl-9211229
ABSTRACT
An enzyme-linked immunosorbent assay (ELISA) based on synthetic peptides identical to lentivirus envelope protein amino acid sequences was used to study serologic reactivity of lentivirus-infected domestic cats and nondomestic felids. One feline immunodeficiency virus (FIV) peptide, P237, was consistently recognized by antibodies from FIV-infected cats, but 2 other FIV peptide antigens were not. The molecular basis for this serologic reactivity was examined. Lentivirus-infected nondomestic Felis species reacted intensely with a puma lentivirus (PLV) peptide corresponding to the conserved FIV peptide. However, lentivirus-infected Panthera species, from which a different lentivirus has been isolated, did not react with the PLV. FIV-infected domestic felids also did not have significant reactivity with the PLV peptide. The peptide ELISA is comparable in sensitivity and specificity to western blot analysis and a commercial enzyme immunoassay. Unlike the other assays, however, the peptide ELISA is inexpensive, requires a small amount of serum, enables the study of specific isotype reactivity, and discriminates between antibodies to FIV and those to PLV. Antibody tests based upon the FIV and the PLV peptides should be useful for detecting the possible introduction of FIV into exotic felids or of lentiviruses from nondomestic felids into the domestic cat population.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas del Envoltorio Viral
/
Síndrome de Inmunodeficiencia Adquirida del Felino
/
Infecciones por Lentivirus
/
Virus de la Inmunodeficiencia Felina
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Vet Diagn Invest
Asunto de la revista:
MEDICINA VETERINARIA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Estados Unidos