Superoxide production and antioxidant enzymes in ammonia intoxication in rats.
Free Radic Res
; 27(6): 637-44, 1997 Dec.
Article
en En
| MEDLINE
| ID: mdl-9455699
ABSTRACT
Injection of large doses of ammonium salts lead to the rapid death of animals. However, the molecular mechanisms involved in ammonia toxicity remain to be clarified. We have tested the effect of injecting 7 mmol/kg of ammonium acetate on the production of superoxide and on the activities of some antioxidant enzymes in rat liver, brain, erythrocytes and plasma. Glutathione peroxidase, superoxide dismutase and catalase activities were decreased in liver and brain (both in cytosolic and mitochondrial fractions) and also in blood red cells, while glutathione reductase activity remained unchanged. Superoxide production in submitochondrial particles from liver and brain was increased by more than 100% in both tissues. Both diminished activity of antioxidant enzymes and increased superoxide radical production could lead to oxidative stress and cell damage, which could be involved in the mechanism of acute ammonia toxicity.
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Banco de datos:
MEDLINE
Asunto principal:
Superóxidos
/
Amoníaco
/
Antioxidantes
Límite:
Animals
Idioma:
En
Revista:
Free Radic Res
Asunto de la revista:
BIOQUIMICA
Año:
1997
Tipo del documento:
Article
País de afiliación:
Rusia