Partial characterization of the epitope on excretory-secretory products of Fasciola hepatica recognized by monoclonal antibody ES78.
J Parasitol
; 84(1): 55-61, 1998 Feb.
Article
en En
| MEDLINE
| ID: mdl-9488338
ABSTRACT
This report contains a partial characterization of the epitope recognized by monoclonal antibody (MAb) ES78 produced against excretory-secretory (ES) antigens of Fasciola hepatica. ES78 is currently used for the detection of ES antigens in serum and stool samples of cattle and humans with fasciolosis, using a highly sensitive and specific sandwich enzyme-linked immunosorbent assay (ELISA). The epitope was characterized by periodate oxidation, alkaline borohydride reduction, trichloroacetic acid precipitation, beta-mercaptoethanol treatment, and enzymatic proteolysis. These results, together with those of the 2-site ELISA, lectin immunoassays, and beta-galactosidase digestion, showed that MAb ES78 reacts with a partly protein/partly carbohydrate antigenic determinant that is found on several ES molecules of adult specimens of F. hepatica and contains at least 1 disulfide bond and beta-galactose probably as galactose-beta(1-3)-N-acetylgalactosamine disaccharide.
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Banco de datos:
MEDLINE
Asunto principal:
Anticuerpos Antihelmínticos
/
Fasciola hepatica
/
Anticuerpos Monoclonales
/
Antígenos Helmínticos
Límite:
Animals
Idioma:
En
Revista:
J Parasitol
Año:
1998
Tipo del documento:
Article
País de afiliación:
Cuba