Localization of the apoptosis-inducing activity of lupus anticoagulant in an annexin V-binding antibody subset.
J Clin Invest
; 101(9): 1951-9, 1998 May 01.
Article
en En
| MEDLINE
| ID: mdl-9576760
ABSTRACT
Lupus anticoagulant (LAC) is associated with arterial and venous thrombosis, thrombocytopenia, and recurrent fetal loss. We have reported previously that plasma with LAC activity induces apoptosis in endothelial cells and binds annexin V (Nakamura, N., Y. Shidara, N. Kawaguchi, C. Azuma, N. Mitsuda, S. Onishi, K. Yamaji, and Y. Wada. 1994. Biochem. Biophys. Res. Commun. 2051488-1493). In this study, we separated two IgG antibody fractions, one with and one without affinity for annexin V, from 10 patients with LAC. LAC and apoptotic activities were localized in the annexin V-binding fraction in all 10 patients. DNA fragmentation was dose-dependent, paralleling the amount of IgG added to the human umbilical vein endothelial cell culture medium, and was inhibited by preincubation with annexin V. Removal of the antiphospholipid antibodies from patient IgG with phospholipid liposomes did not abolish the apoptosis-inducing activities or binding to annexin V. These results imply that patients with LAC often have antibodies that do not bind phospholipids and are responsible for the induction of apoptosis in endothelial cells.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Endotelio Vascular
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Inhibidor de Coagulación del Lupus
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Apoptosis
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Anexina A5
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Lupus Eritematoso Sistémico
Límite:
Adult
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Female
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Humans
Idioma:
En
Revista:
J Clin Invest
Año:
1998
Tipo del documento:
Article
País de afiliación:
Japón