Ganglioside GM2 is substrate for a sialidase in MDCK cells.
FEBS Lett
; 428(1-2): 115-7, 1998 May 22.
Article
en En
| MEDLINE
| ID: mdl-9645488
ABSTRACT
GM1 ganglioside carrying a fluorescent fatty acid in substitution of the natural one, has been administered to cultured Madin-Darby canine kidney (MDCK) cells for different pulse times (0.5-24 h), and its metabolic fate was followed. The fluorescent GM2, asialo-GM2, asialo-GM1 and ceramide were the only detectable metabolites. The complete absence of fluorescent GM3 is consistent with the presence in these cells of a sialidase working on GM1 and GM2 gangliosides. After treatment of the cells with chloroquine the fluorescent GM1 remained essentially undegraded, indicating a catabolic processing at lysosomal level.
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Banco de datos:
MEDLINE
Asunto principal:
Gangliósido G(M2)
/
Neuraminidasa
Límite:
Animals
Idioma:
En
Revista:
FEBS Lett
Año:
1998
Tipo del documento:
Article
País de afiliación:
Italia