pH-regulated electroretention chromatography: towards a new method for the separation of proteins according to their isoelectric points.
Electrophoresis
; 19(7): 1135-9, 1998 Jun.
Article
en En
| MEDLINE
| ID: mdl-9662175
ABSTRACT
The pH-dependent electroretention behavior of model proteins cytochrome c and ribonuclease A was studied in a hollow fiber arrangement, similar to that used in electrical field-flow fractionation. Field-induced immobilization of the proteins at the inner wall of the fiber was a function of the pH adjusted in the solution surrounding it, indicating that the pH inside the fiber lumen, relevant for protein migration, quickly equilibrates to the regulated value outside. A complete separation of the model proteins was achieved. Advantages of the principle as well as prospects for the development of a technique separating more than two protein species according to their isoelectric points are discussed.
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Banco de datos:
MEDLINE
Asunto principal:
Proteínas
/
Cromatografía
Límite:
Animals
Idioma:
En
Revista:
Electrophoresis
Año:
1998
Tipo del documento:
Article
País de afiliación:
Alemania