Influence of photobiomodulation therapy on root development of rat molars with open apex and pulp necrosis
Braz. oral res. (Online)
; 33: e084, 2019. graf
Article
em En
| LILACS
| ID: biblio-1019612
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract This study aimed to evaluate the role of photobiomodulation (PBM) in apexification and apexogenesis of necrotic rat molars with an open apex. Rat molars were exposed to the oral environment for 3 weeks. Canals were rinsed with 2.5% NaOCl and 17% EDTA, filled with antibiotic paste and sealed. After 7 days, canals were rinsed and divided into six groups (n=6) mineral trioxide aggregate (MTA); blood clot (BC); human dental pulp stem cells (hDPSC); MTA+PBM; BC+PBM; and hDPSC+PBM. In hDPSC groups, a 1% agarose gel scaffold was used. Two groups were not exposed healthy tooth+PBM (n = 6), healthy tooth (n = 3); and one was exposed throughout the experiment necrotic tooth (n = 3). In PBM groups, irradiation was performed with aluminum gallium indium phosphide (InGaAlP) diode laser for 30 days within 24-h intervals. After that, the specimens were processed for histological and immunohistochemical analyses. Necrotic tooth showed greater neutrophil infiltrate (p < 0.05). Necrotic tooth, healthy tooth, and healthy tooth+PBM groups showed absence of a thin layer of fibrous condensation in the periapical area. All the other groups stimulated the formation of a thicker layer of fibers (p < 0.05). All groups formed more mineralized tissue than necrotic tooth (p < 0.05). PBM associated with MTA, BC, or hDPSC formed more mineralized tissue (p < 0.05). MTA+PBM induced apexification (p < 0.05). Rabbit polyclonal anti-bone sialoprotein (BSP) antibody confirmed the histological findings of mineralized tissue formation, and hDPSC groups exhibited higher percentage of BSP-positive cells. It can be concluded that PBM improved apexification and favored apexogenesis in necrotic rat molars with an open apex.
Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Doenças Dentárias
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Necrose da Polpa Dentária
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Ápice Dentário
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Terapia com Luz de Baixa Intensidade
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Cavidade Pulpar
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Lasers Semicondutores
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Apexificação
Limite:
Animals
Idioma:
En
Revista:
Braz. oral res. (Online)
Assunto da revista:
ODONTOLOGIA
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil