Performance of direct immunofl uorescence assay for the detection of human metapneumovirus under clinical laboratory settings
Rev. Soc. Bras. Med. Trop.(online);
48(6): 762-764, Nov.-Dec. 2015. tab
Artigo
em Inglês
| Coleciona SUS - BR, CONASS, SES-RS | ID: biblio-1122026
Introduction:
Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofl uorescence (DIF) to detect hMPV in a clinical laboratory setting.Methods:
Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF and real time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays.Results:
In all, 36 (8%) samples were positive by DIF and 94 (21%) were positive by qRT-PCR. Direct immunofl uorescence specifi city was 99% and sensitivity was 38%.Conclusions:
DIF is not very sensitive under clinical laboratory settings. (AU)
Biblioteca responsável:
BR1618.1
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