Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca
Braz. arch. biol. technol
; 64: e21200316, 2021. tab, graf
Article
em En
| LILACS
| ID: biblio-1278451
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.
Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Plantas Geneticamente Modificadas
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Arabidopsis
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Fragaria
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Herbicidas
Idioma:
En
Revista:
Braz. arch. biol. technol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China