New strategy to improve quality control of Montenegro skin test at the production level
Rev. Soc. Bras. Med. Trop
; 50(6): 788-794, Nov.-Dec. 2017. tab, graf
Article
em En
| LILACS
| ID: biblio-897029
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
The production of the Montenegro antigen for skin test poses difficulties regarding quality control. Here, we propose that certain animal models reproducing a similar immune response to humans may be used in the quality control of Montenegro antigen production.METHODS:
Fifteen Cavia porcellus (guinea pigs) were immunized with Leishmania amazonensis or Leishmania braziliensis , and, after 30 days, they were skin tested with standard Montenegro antigen. To validate C. porcellus as an animal model for skin tests, eighteen Mesocricetus auratus (hamsters) were infected with L. amazonensis or L. braziliensis , and, after 45 days, they were skin tested with standard Montenegro antigen.RESULTS:
Cavia porcellus immunized with L. amazonensis or L. braziliensis , and hamsters infected with the same species presented induration reactions when skin tested with standard Montenegro antigen 48-72h after the test.CONCLUSIONS:
The comparison between immunization methods and immune response from the two animal species validated C. porcellus as a good model for Montenegro skin test, and the model showed strong potential as an in vivo model in the quality control of the production of Montenegro antigen.Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Leishmania braziliensis
/
Testes Intradérmicos
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Leishmaniose Cutânea
/
Modelos Animais
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Antígenos de Protozoários
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Brasil