Immunoexpression of vascular endothelial growth factor and B-cell lymphoma 2 in the uterine tissue of rats treated with melatonin in the estrus phase
Acta cir. bras
; 33(7): 629-640, July 2018. tab, graf
Article
em En
| LILACS
| ID: biblio-949364
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To investigate the effect of melatonin on uterine tissue in the ovariectomized rat model.Methods:
Fourty Wistar albino rats were divided into four groups for histologic and immunohistochemical examination. The rats were first numbered randomly and then randomly divided into 4 equal groups control (group 1), torsion (group 2), torsion+detorsion (group 3) and torsion+detorsion+melatonin (group 4) groups. In addition, four Wistar albino rats were used for western blot analysis in each group. And also, malondialdehyde (MDA) levels were measured biochemically in all rats.Results:
The histopathological examination of the uterine tissue in rats ovarectomized showed a degeneration in uterine glands, dilation of blood vessels in the internal layer with a thrombosis and bleeding, abnormal nucleuses and vacuolated cytoplasm above and below the nucleus. In torsion group, the apoptotic cells increased in luminal epithelium and gland cells. In the melatonin group showed that the Bcl2 negative effect on the uterine epithelium and did not lead to apoptotic cells.Conclusion:
The increase in vascular endothelial growth factor expression resulted in the rearrangement of endothelial cell growth and the induction of angiogenesis.Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Útero
/
Estro
/
Genes bcl-2
/
Fator A de Crescimento do Endotélio Vascular
/
Melatonina
/
Antioxidantes
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Article
País de afiliação:
Turquia