Effects of alpha-tocopherol on bacterial translocation and lipid peroxidation in rats with intestinal obstruction
Acta cir. bras
; 18(4): 283-288, jul.-ago. 2003. ilus, tab
Article
em En
| LILACS
| ID: lil-347140
Biblioteca responsável:
BR1.2
RESUMO
PURPOSE:
Investigate if alpha-tocopherol has a protective effect on intestinal mucosa after obstruction and to evaluate the potential relations between lipid peroxidation and bacterial translocation.METHODS:
Ten rats were submitted to a sham laparotomy and six served as control group. A small bowel obstruction was done in sixteen animals and among them eight were pretreated with alpha-tocopherol. Forty-eight hours later, mesenteric lymph node, spleen, liver and blood cultures and also samples from ileal mucosal were obtained, Thiobarbituric acid reactive substances (TBARS) levels were determined and intestinal histological assessment was performed.RESULTS:
Bacterial translocation was significantly increased in the obstructed rats compared with the control, sham and antioxidant pretreated groups (p< 0,05). TBARS (nmol/100mg) in untreated obstructed rats increased from 49,0 ± 13,3 in control group to 128,8 ± 40 after 48 hours of intestinal obstruction and achieved 72,3 ± 24,6 in alpha-tocopherol group (p< 0,05). Bacterial adherence to the intestinal epithelial cells surface and mucosal necrosis were significantly increased in the obstructed compared with nonobstructed rats.CONCLUSION:
Alpha-tocopherol reduce the deleterious effects of the TBARS over the intestinal mucosal suggesting that in such circumstances there might be an association between bacterial translocation and lipid peroxidation after an intestinal occlusion.
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Vitamina E
/
Peroxidação de Lipídeos
/
Translocação Bacteriana
/
Radicais Livres
/
Obstrução Intestinal
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil