Prevalence of cagA and vacA genes in isolates from patients with Helicobacter pylori-associated gastroduodenal diseases in Recife, Pernambuco, Brazil
Mem. Inst. Oswaldo Cruz
; 98(6): 817-821, Sept. 2003. ilus, tab
Article
em En
| LILACS
| ID: lil-348352
Biblioteca responsável:
BR1.1
ABSTRACT
Geographical differences in the prevalence of Helicobacter pylori genes and their association with disease severity have been identified. This study analyzes the prevalences of the cagA gene and alleles of the vacA gene in H. pylori-associated gastroduodenal diseases in isolates from Recife, PE, Brazil. Gastric biopsy of 61 H. pylori-positive patients were submitted to DNA extraction and gene amplification by polymerase chain reaction. Among the 61 patients, 21 suffered from duodenal ulcer (DU) and 40 from gastritis (GT). The prevalence of H. pylori strains harbouring the cagA gene was higher in the DU group (90.5 percent) than in the GT group (60 percent) (p = 0.02). The vacA gene was amplified in 56 out of 61 biopsies, of which 43 (76.8 percent) contained bacteria carrying the s1 allele and 13 (23.2 percent) the s2. However, the prevalence of the vacA s1 genotying was the same in either DU or GT group. The majority of the s1-typed strains, 39 (90.7 percent) out of 43, were subtype s1b. In resume there was a strong association between the H. pylori cagA+ gene and DU. However, there were no differences between the DU and GT groups in relation to the vacA s1 and s2 alleles distribution, albeit the subtype s1b was predominat
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Helicobacter pylori
/
Infecções por Helicobacter
/
Úlcera Duodenal
/
Gastrite
Tipo de estudo:
Prevalence_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Brasil