In vitro antiproliferative effect of fractions from the caribbean marine sponge Myrmekioderma gyroderma / Efecto antiproliferativo in vitro de fracciones obtenidas en un extracto de la esponja marina Myrmekioderma gyroderma existente en el mar Caribe
Rev. cuba. farm;
47(3): 368-378, jul.-sep. 2013.
Artigo
em Inglês
| LILACS | ID: lil-691246
Introduction:
studies performed to Myrmekioderma genus sponges show phospholipid fatty acids, volatile compounds, sterols, bioactive cyclic diterpenes, sesquiterpenes, lineal diterpenes and glycolipid ethers. Objetive to evaluate the antiproliferative effect of seven fractions (F1-F7) obtained by flash column chromatography from the most bioactive extract of the sponge Myrmekioderma gyroderma, and to analyze the chemical composition of the most active fraction.Methods:
samples of dried sponge were extracted with two different solvents CH2Cl2 (2 x 50 mL), and CH3OH (2 x 50 mL). Each fraction was evaluated on tumor cell derived cell lines; and the cell growth, and viability were determined by a colorimeter assay using sulforhodamine B. Fatty acids structure of the most active fraction was possible by GC-MS analysis of the methyl ester, and pyrrolidine derivatives.Results:
the fraction with higher activity on the assessed tumor cell lines is F4 due to it totally inhibited MDA-MB-231, and HT29 cell line growth to 5, and 25 µg/mL concentration (IC50< 1 µg/mL). Fatty acids identified in bioactive F4 fraction of the M. gyroderma sponge can be classified on the following groups lineal chain saturated, branched-saturated, unsaturated, and a 3-hydroxy acid.Conclusions:
43 fatty acids among saturated, branched-saturated, and unsaturated were identified out of the F4 fraction with activity on the cell lines derived of breast cancer MDA-MB-231, colon carcinoma HT29, and lung carcinoma cells A-549. These results show the growth inhibitory effect shown by the fractions, on the tumor cell lines, depends on the dose.
Biblioteca responsável:
CU1.1
Texto completo
Similares
