Alterations in the duodenum myenteric neurons of Wistar ratsafter ingesting of 2, 4 dichlorophenoxyacetic acid
Braz. j. morphol. sci
; 30(1): 28-32, 2013. tab, ilus
Article
em En
| LILACS
| ID: lil-699325
Biblioteca responsável:
BR734.1
ABSTRACT
The 2,4 dichlorophenoxyacetic acid (2,4-D) is a systemic herbicide whose effects in animal organic systemshave been examined in previous studies, being the neurotoxicity considered the predominant effect. However,the studies that detect the 2,4-D neurotoxicity have merely focused in the central nervous system, andtherefore, little is known about the effect of this herbicide in the enteric nervous system. This study aimedto verifying the 2,4-D effects on the myenteric neurons in duodenum of Wistar rats. Ten 60-day-old maleWistar rats (Rattus norvegicus) were divided in two groups control group (C) that did not receive 2,4-D andexperimental group (E) that received 5.0 mg of 2,4-D/kg for 15 days. At the end of experimental period, theanimal were euthanized, the duodenum was collected and processed for NADPH-diaphorase histochemicalanalysis in order to expose the nitrergic myenteric neurons (NADPH-dp). In the light microscopy analysis, thewhole-mount preparation obtained from duodenum of each animal were image-captured in 120 and 40 fields,for quantitative and morphometric analyses of myenteric neurons, respectively. The neuronal density was notaffected when comparing the two groups, but an increase (p > 0.05) of 8.5% was observed in the cell bodyarea of neurons in the E group. In conclusion, the ingestion of 2,4-D at a dosage of 5.0 mg/kg body weightfor 15 days does not change the neuronal density, but promotes the hypertrophy of NADPH-dp myentericneurons in duodenum of the rats of this study.
Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Neurônios Nitrérgicos
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Herbicidas
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Intestino Delgado
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Plexo Mientérico
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NADPH Desidrogenase
Limite:
Animals
Idioma:
En
Revista:
Braz. j. morphol. sci
Assunto da revista:
ANATOMIA
Ano de publicação:
2013
Tipo de documento:
Article
País de afiliação:
Brasil