DNA prime-protein boost based vaccination with a conserved region of leptospiral immunoglobulin-like A and B proteins enhances protection against leptospirosis
Mem. Inst. Oswaldo Cruz
; 110(8): 989-995, Dec. 2015. tab, graf
Article
em En
| LILACS
| ID: lil-769835
Biblioteca responsável:
BR1.1
ABSTRACT
Leptospirosis is a zoonotic disease caused by pathogenic spirochetes of theLeptospira genus. Vaccination with bacterins has severe limitations. Here, we evaluated the N-terminal region of the leptospiral immunoglobulin-like B protein (LigBrep) as a vaccine candidate against leptospirosis using immunisation strategies based on DNA prime-protein boost, DNA vaccine, and subunit vaccine. Upon challenge with a virulent strain ofLeptospira interrogans, the prime-boost and DNA vaccine approaches induced significant protection in hamsters, as well as a specific IgG antibody response and sterilising immunity. Although vaccination with recombinant fragment of LigBrep also produced a strong antibody response, it was not immunoprotective. These results highlight the potential of LigBrep as a candidate antigen for an effective vaccine against leptospirosis and emphasise the use of the DNA prime-protein boost as an important strategy for vaccine development.
Palavras-chave
Texto completo:
1
Bases de dados:
LILACS
Assunto principal:
Proteínas de Bactérias
/
Vacinas Bacterianas
/
Vacinação
/
Leptospira
/
Leptospirose
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Antígenos de Bactérias
Limite:
Animals
Idioma:
En
Revista:
Mem. Inst. Oswaldo Cruz
Assunto da revista:
MEDICINA TROPICAL
/
PARASITOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil