Immunochemical localization of the fraction-1 antigen, a virulence determinant of Yersinia pestis
An. acad. bras. ciênc
; 62(3): 291-7, set. 1990. ilus
Article
em En
| LILACS
| ID: lil-92525
Biblioteca responsável:
BR1.1
ABSTRACT
The distribution of Yersina pestis Fraction-1 (F1) antigen was analyzed in cells grown at 28§C and 37§C. Fractionation of Y. pestis cells followed by analysis in SDS-polyacrylamide gel electrophoresis indicated that the mature form of the F1 antigen is localized in the extracellular matrix and in the cytoplasm. Localization of the F1 antigen was confirmed by immunoblots and a single peptide with a molecular weight of 17,000 daltons was recognized. Electron microscopy of Y. pestis cells labeled with colloidal gold-conjugated antibodies corroborated the extracellular matrix and cytoplasm dual location of the F1 antigen
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Bases de dados:
LILACS
Assunto principal:
Yersinia pestis
/
Fracionamento Celular
/
Antígenos de Bactérias
Idioma:
En
Revista:
An. acad. bras. ciênc
Assunto da revista:
CIENCIA
Ano de publicação:
1990
Tipo de documento:
Article