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1.
Vet Immunol Immunopathol ; 187: 14-19, 2017 May.
Artículo en Inglés | MEDLINE | ID: mdl-28494924

RESUMEN

Canine T-zone lymphoma (TZL) is a peculiar lymphoma subtype characterized by an indolent clinical course and aberrant CD45-negative phenotype, commonly recognized by flow cytometry (FC). Recent studies have described clinical presentation and behavior, but to date the mechanisms behind the loss of CD45 protein expression have never been investigated. The aims of this study were: 1) to confirm the absence of CD45 in canine TZL via the concomitant use of FC and immunohistochemistry with two different sources of antibody; and 2) to investigate the amount of CD45 transcript and the presence of CD45 gene in the neoplastic cells of dogs affected by TZL. 57 lymph node aspirates were included in the present study: 40 (70.2%) TZLs, 7 (12.3%) high grade T-cell lymphomas and 10 (17.5%) reactive lymph nodes. Neoplastic cells and normal T-cells were isolated from TZL and reactive lymph nodes, respectively, via cell sorting. Immunohistochemistry was performed on 2 TZL, 2 reactive lymph nodes and 2 Peripheral T-cell Lymphomas. Total RNA and genomic DNA were extracted from lymph-nodes aspirates. Two different quantitative real-time PCR experiments were designed, to determine the amount of the CD45 transcript and of the corresponding gene fragment. All TZL cases were negative for CD45 at immunohistochemistry. CD45 transcript amount was significantly lower in TZL compared to controls (p<0.001). This difference was not significant (p=0.584) for CD45 DNA load, that was similar between TZL and controls. Moreover, CD45 transcript amount was inversely correlated with the percentage of neoplastic cells in each TZL sample (p=0.010). These results confirm that CD45 protein is lacking on cell surface irrespective of the technique and antibody source adopted. This phenotypic aberrancy is apparently due to the absence of gene transcription, as CD45 DNA was present, whereas CD45 transcript was virtually absent in the neoplastic cells. The data here reported support further studies investigating possible factors impairing CD45 gene transcription.


Asunto(s)
Enfermedades de los Perros/metabolismo , Antígenos Comunes de Leucocito/metabolismo , Linfoma de Células T/veterinaria , Animales , Enfermedades de los Perros/inmunología , Enfermedades de los Perros/patología , Perros , Citometría de Flujo/veterinaria , Regulación Neoplásica de la Expresión Génica , Linfoma de Células T/inmunología , Linfoma de Células T/metabolismo , Linfoma de Células T/patología , Reacción en Cadena en Tiempo Real de la Polimerasa/veterinaria
2.
Vet Comp Oncol ; 15(2): 366-373, 2017 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-26463596

RESUMEN

Richter's syndrome (RS) is the development of an aggressive lymphoma in patients with chronic lymphocytic leukaemia (CLL). In humans, RS occurs in 2-20% of CLL, which transform into diffuse large B-cell lymphoma but reports in dogs are scarce. This study retrospectively describes eight dogs with CLL progressing into RS. A database including 153 dogs with CLL (93T CD8+ and 55 B-CLL) was interrogated and RS was demonstrated in eight cases (representing 5.2% of total CLL): two with T-cell (2.2% of T CLL) and six with a B-cell immunophenotype (10.9% of B-CLL). When RS occurred, lymphocytes were decreased compared to CLL. Five dogs had anaemia and two dogs thrombocytopenia. Frequent clinical signs included lymph node swelling, coughing, vomiting, neurological signs and weight loss. Independently from the therapy, RS was associated with a short survival (median 41 days). RS should be considered as an unfavourable evolution in canine CLL.


Asunto(s)
Transformación Celular Neoplásica/patología , Enfermedades de los Perros/patología , Leucemia Linfocítica Crónica de Células B/veterinaria , Linfoma no Hodgkin/veterinaria , Animales , Perros , Femenino , Citometría de Flujo/veterinaria , Leucemia Linfocítica Crónica de Células B/patología , Recuento de Linfocitos/veterinaria , Linfoma no Hodgkin/patología , Masculino , Estudios Retrospectivos
3.
Vet Immunol Immunopathol ; 142(3-4): 228-35, 2011 Aug 15.
Artículo en Inglés | MEDLINE | ID: mdl-21663977

RESUMEN

MicroRNAs (miRNAs) are posttranscriptional regulatory noncoding RNAs used to profile human hematopoietic tumors. In this study, some mature miRNAs was quantitated in peripheral blood from dogs with chronic lymphocytic leukemia (CLL). Relative expression data were normalised against four endogenous controls (let-7a, miR-17-5p, miR-26b, and miR-223) selected by geNorm analysis. The results revealed distinct miRNA patterns in CLL depending on the immunophenotype. Also in dogs, the different miRNAs expression could reflect developmental lineage and tumor differentiation. The similar genetics, physiology and exposure to environment in dogs and humans make the miRNA expression study in canine CLL attractive for comparative oncology.


Asunto(s)
Enfermedades de los Perros/inmunología , Regulación Leucémica de la Expresión Génica/inmunología , Leucemia Linfocítica Crónica de Células B/veterinaria , MicroARNs/inmunología , Animales , Enfermedades de los Perros/genética , Perros , Citometría de Flujo/veterinaria , Inmunofenotipificación/métodos , Inmunofenotipificación/veterinaria , Leucemia Linfocítica Crónica de Células B/genética , Leucemia Linfocítica Crónica de Células B/inmunología , MicroARNs/biosíntesis , MicroARNs/genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/veterinaria , Estadísticas no Paramétricas
4.
Leuk Res ; 34(8): 1070-7, 2010 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-19945163

RESUMEN

The elucidation of microRNA (miRNA) expression pattern in canine lymphoma is attractive for veterinary and comparative oncology due to similar genetics, physiology and exposure to environment in dogs and humans. In this work, the expression of a panel of mature miRNAs was quantitated in fresh-frozen and formalin-fixed paraffin-embedded (FFPE) lymph nodes from canine lymphoma. The major findings were: the detection of a panel of miRNAs expressed in canine lymph node; the identification of three suitable endogenous controls (let-7a, miR-16, and miR-26b) by NormFinder and geNorm analysis; the concordance between results obtained from fresh-frozen and FFPE samples; the detection of upregulation of miR-17-5p and miR-181a in B- and T-cell lymphomas respectively. This is the first study aimed to the application of miRNAs analysis in canine lymphoma.


Asunto(s)
Biomarcadores de Tumor/genética , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/patología , Linfoma/genética , MicroARNs/análisis , Animales , Perros , Formaldehído/química , Secciones por Congelación , Linfoma/patología , MicroARNs/fisiología , Análisis de Secuencia por Matrices de Oligonucleótidos , Adhesión en Parafina , Fijación del Tejido
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