MMP-9 Levels in the Gingival Crevicular Fluid of Chilean Rosacea Patients.

Rosacea is a chronic inflammatory skin disease whose prevalence rates remain unknown in Chile. Laboratory benchmark testing for this disease is not useful, therefore, we aimed to evaluate the gingival crevicular fluid (GCF) levels of extracellular metalloproteinases (MMP)-2 and MMP-9 as novel rosacea biomarkers. We designed a cross-sectional study with a control group. Participants were systemically healthy adults (n = 20) and persons with rosacea (n = 18). We performed a periodontal evaluation and collected gingival crevicular fluid to measure MMP-2 and MMP-9 levels. Analysis showed mean and standard deviation of MMP-9 concentrations in the GCF for patients with rosacea was 764.52 ± 569.83 pg/mL; for healthy patients, it was 260.69 ± 170.43 pg/mL (p < 0.05). The diagnosis of rosacea was responsible for the levels of MMP-9 in the GCF (p < 0.05), as opposed to periodontitis, smoking, and age (p > 0.05). The Area under ROC for MMP-9 was 0.869 (95%, C.I: 0.719−0.956), with a sensitivity of 72.22% and specificity of 81.58% for the diagnosis of rosacea. We conclude that the quantification of MMP-9 in the GCF could be used as a biomarker of rosacea. Also, rosacea was responsible for increasing the levels of MMP-9 in the GCF independent of periodontal status.

Relaxin Inhibits the Cardiac Myofibroblast NLRP3 Inflammasome as Part of Its Anti-Fibrotic Actions via the Angiotensin Type 2 and ATP (P2X7) Receptors.

Chronic NLRP3 inflammasome activation can promote fibrosis through its production of interleukin (IL)-1ß and IL-18. Conversely, recombinant human relaxin (RLX) can inhibit the pro-fibrotic interactions between IL-1ß, IL-18 and transforming growth factor (TGF)-ß1. Here, the broader extent by which RLX targeted the myofibroblast NLRP3 inflammasome to mediate its anti-fibrotic effects was elucidated. Primary human cardiac fibroblasts (HCFs), stimulated with TGF-ß1 (to promote myofibroblast (HCMF) differentiation), LPS (to prime the NLRP3 inflammasome) and ATP (to activate the NLRP3 inflammasome) (T+L+A) or benzoylbenzoyl-ATP (to activate the ATP receptor; P2X7R) (T+L+Bz), co-expressed relaxin family peptide receptor-1 (RXFP1), the angiotensin II type 2 receptor (AT2R) and P2X7R, and underwent increased protein expression of toll-like receptor (TLR)-4, NLRP3, caspase-1, IL-1ß and IL-18. Whilst RLX co-administration to HCMFs significantly prevented the T+L+A- or T+L+Bz-stimulated increase in these end points, the inhibitory effects of RLX were annulled by the pharmacological antagonism of either RXFP1, AT2R, P2X7R, TLR-4, reactive oxygen species (ROS) or caspase-1. The RLX-induced amelioration of left ventricular inflammation, cardiomyocyte hypertrophy and fibrosis in isoproterenol (ISO)-injured mice, was also attenuated by P2X7R antagonism. Thus, the ability of RLX to ameliorate the myofibroblast NLRP3 inflammasome as part of its anti-fibrotic effects, appeared to involve RXFP1, AT2R, P2X7R and the inhibition of TLR-4, ROS and caspase-1.

Patient experience and non-surgical periodontal therapy in a postgraduate periodontal training program: A qualitative study

ABSTRACT: Objective: To explore patient experience for non-surgical periodontal therapy using phenomenography. Methods: This qualitative study was performed in a postgraduate specialist training program in periodontics. Patients reported their experiences through in-depth interviews in order to recognize the impact of non-surgical periodontal therapy on their quality of life and their satisfaction. A homogeneous sampling procedure was used, establishing redundancy after the fifteenth interview. Interviews were audio recorded, transcribed and analyzed by three researchers who codified the answers into categories to determine the emerging topics. Results: The core emerging themes were: (i) lack of information about periodontal disease; (ii) experience during treatment; (iii) treatment implications; and (iv) clinician-patient relationship. Conclusions: Patients seem to have limited knowledge about periodontal diseases. Reasons for attendance mainly include bleeding gums, tooth mobility, and aesthetic problems. Therapy can generate pain, fear, and is considered invasive, whereas dentine hypersensitivity may occur post-operatively. Despite this, patients would be willing to undergo treatment again and were generally satisfied with the level of care and treatment received.

Enseñanza histológica e histopatológica a través de microscopía virtual, una oportunidad en odontología / Virtual microscopy in histological and histopathological teaching, an opportunity in dentistry

RESUMEN La microscopía virtual (MV) está siendo ampliamente implementada en educación y podría llevar a reemplazar a la microscopía óptica (MO). Objetivo: Proporcionar una revisión de la literatura a partir de las preguntas ¿Cuál es la percepción de académicos y estudiantes? y ¿Cuál es el desempeño de los estudiantes? respecto a la enseñanza de histología y/o histopatología con MV en odontología. Material y métodos: Se consultaron las bases de datos: Pubmed, Scielo, Science Direct y Scopus, y 10 artículos fueron seleccionados. Resultados: La totalidad de estudios que evaluaron percepción y desempeño académico obtuvieron resultados a favor de la MV. Conclusiones: La MV tiene un futuro prometedor, pero más estudios con metodologías similares y que consideren la percepción de los académicos son requeridos.

ABSTRACT Virtual microscopy (VM) is being widely implemented in education and could lead to the replacement of light microscopy (LM). Objective: To provide a review of the literature based on the questions: What is the perception of academics and students? and What is the performance of students? regarding the teaching of histology and/or histopathology with VM in dentistry. Material and methods: The following databases were consulted: Pubmed, Scielo, Science Direct and Scopus, and 10 articles were selected. Results: All the studies that evaluated perception and academic performance obtained results in favor of VM. Conclusions: VM has a promising future, but more studies with similar methodologies and that consider the perception of academics are required.

Utilidad de tinción de tricrómico de Masson en la cuantificación de densidad media vascular en mucosa oral normal, displasia epitelial y carcinoma oral de células escamosas / Utility of Masson's trichrome stain in the quantification of mean vascular density in normal oral mucosa, epithelial dysplasia and oral squamous cell carcinoma

RESUMEN: El objetivo de este estudio fue valuar la utilidad del uso de la tinción de Tricrómico de Masson (TM) en la cuantificación de la densidad media vascular (DMV) en Mucosa Oral Normal (MON), Displasia Epitelial Oral (DEO) y Carcinoma Oral de Células Escamosas (COCE). Estudio descriptivo de serie de casos. Se analizaron 17 muestras de MON, 15 muestras de DEO y 16 de COCE, teñidas con TM. Para determinar su utilidad, se compararon con las mismas muestras analizadas con técnica de inmunohistoquímica contra CD31. La cuantificación de la DMV se realizó en las 3 áreas de mayor vascularización de cada muestra. Se determinó la DMV según diagnóstico mediante la tinción TM e inmunohistoquímica contra CD31, y se calculó la correlación entre ambos. La DMV cuantificada con TM y contra CD31 difiere según el diagnóstico, observándose un aumento de la DMV al malignizarse el diagnóstico. No se encontraron diferencias al comparar la DMV cuantificada con TM y contra CD31. La correlación de la DMV analizado por TM y contra CD31 es significativa y moderada. La cuantificación de vasos sanguíneos es posible mediante la tinción de TM en muestras de MON, DEO y COCE, con una correlación moderada con la inmunohistoquímica contra CD31.

SUMMARY. The objective of this study was to evaluate the utility of Masson's Trichrome (TM) staining in the quantification of the mean vascular density (DMV) in samples of normal oral mucosa (MON), oral epithelial dysplasia (ODE) and oral squamous cell carcinoma (COCE). The design - a descriptive study of case series. We analyzed 17 samples of MON, 15 samples of DEO and 16 samples of COCE, stained with TM. To determine usefulness, we compared and analyzed the same samples, either stained with TM or with immunohistochemical technique against CD31. Quantification of the DMV was performed in the 3 areas of greatest vascularization in each sample. DMV was determined according to diagnosis by TM staining and immunohistochemistry against CD31, and the correlation between the two was then calculated. DMV quantified with TM and against CD31 differs according to the diagnosis, with an increase in DMV upon malignant diagnosis. No differences were found when comparing DMV quantified with TM and against CD31. The correlation of the DMV analyzed by TM and against CD31 is significant and moderate. Quantification of blood vessels is possible by TM staining in samples of MON, DEO and COCE. TM staining is moderately correlated with immunohistochemistry against CD31.

La superación profesional del técnico en salud y el empleo de los entornos virtuales de enseñanza / The health technician's professional training and the use of the teaching virtual environments

Se propone una estrategia, desde un enfoque desarrollador del proceso de enseñanza-aprendizaje, con vistas a contribuir a la superación profesional de los docentes de los institutos superiores tecnológicos en relación con el uso de los entornos virtuales, lo cual les permitirá responder al desafío transformador de este nivel educativo.

A strategy of the teaching-learning process is proposed from a developing approach, with the aim of contributing to the professional training of the teaching staff of the technological higher institutes in relation to the use of the virtual environments, which will allow them to respond to the transforming challenge of this educational level.

Presencia y morfometría de forámenes y canales linguales en cone beam CT de mandíbulas de individuos chilenos: correlación con medición directa en hueso / Presence and morphometry of lingual foramina and canal in cone beam CT of Chilean dry mandibles: correlation with direct bone measures

Se han descrito forámenes ubicados en la cara interna del sector mandibular anterior, ya sea en hueso seco, radiografía convencional y en estudios mediante cone beam CT (CBCT). El objetivo de este estudio fue comparar la presencia, posición y las características anatómicas de los forámenes y canales linguales encontrados en CBCT de mandíbulas Chilenas secas, con los resultados de la misma muestra estudiada con visión directa en hueso seco. Se utilizaron 68 mandíbulas secas de la unidad de morfología de la Universidad de los Andes. Se evaluó número de dientes remanentes, número de forámenes relacionados con las espinas mentonianas, posición, área y longitud de cada canal en hueso seco y en CBCT de la misma muestra. Se encontró presencia del foramen lingual en 100 % de CBCT y 97 % de observación directa en hueso. Se observaron 150 forámenes en hueso seco y 170 forámenes en CBCT. La mayor cantidad se encontró por superior (hueso: 55; CBCT:61) e inferior (hueso:66 ; CBCT:92) a las espinas mentonianas. La confiablidad no fue la esperada, con un kappa para el número de forámenes superiores de 0,43, una correlación intraclase (rho) para el número de forámenes inferiores de 0,31. El área de forámenes superiores tuvo un rho de 0,059 e inferiores un rho de 0,144, mientras que, la longitud de los canales superiores, un rho de 0,5 e inferiores un rho de 0,246. Se observó una tendencia a mayores diámetros y longitudes de canales en las mediciones realizadas en CBCT comparadas con las mediciones en hueso seco. Teniendo en cuenta la alta prevalencia de estos forámenes, es recomendable previo a cualquier cirugía en el sector mandibular anterior, el estudio mediante CBCT. Además de mantener un margen de seguridad respecto a los forámenes linguales debido a una mínima distorsión esperable de estas estructuras.

On several studies, there has been described foramina located in the inner face of the anterior mandible, whether on dry bone, conventional radiography and cone beam CT (CBCT). The aim of this study was compare the presence, position and anatomical characteristics of foramina and lingual canals found in CBCT of Chilean mandibles, with the results of the same sample studied with direct vision in dry bone. We studied 68 mandibles from the morphology unit of Universidad de los Andes. The number of teeth, foramina related to mental spines, position, area and length of each canal were evaluated, in dry bone and CBCT from the same sample. Presence of lingual foramen was found in 100 % of CBCT and 97 % of direct observation in bone. There were 150 foramina in dry bone and 170 foramina in CBCT. The greatest amount was found superior (bone: 55; CBCT: 61) and inferior (bone: 66; CBCT: 92) to the mental spines. . The reliability was not as expected, with a kappa for the number of upper foramina of 0.43, an intraclass correlation for the number of lower foramen of 0.31. The upper foramen area had an intraclass correlation of 0,059 and an inferior correlation of 0.144 while, the length of the superior canals, had a 0.5 and inferior 0.246 of intraclass correlation. A trend was observed for higher diameters and canal lengths in the measurements performed with CBCT compared to the measurements in dry bone. Taking into account the high prevalence of these foramina, it is advisable prior to any surgery in the anterior mandible, the prior study using CBCT. In addition to maintaining a margin of safety with respect to lingual foramina.

Frequency and characterization of the infratemporal spine in a sample of Chilean human skulls / Frecuencia y caracterización de la espina infratemporal en una muestra de cráneos humanos chilenos

The infratemporal spine, or sphenoidal tubercle, is a bony structure described in both classical anatomical literature and contemporary literature. However, the available literature does not mention the specific anatomical characteristics or the distribution of this bony element in the population. The aim of this study was to define this structure, identify its presence, and identify its morphology in a sample of Chilean human skulls. Fifty-seven dry skulls, obtained from the morphology unit at Universidad de los Andes, were used. The great wings of the sphenoid bone on both sides of the skull were evaluated in search of the infratemporal spine. These spines were classified according to their morphological characteristics of either laminar, pyramidal, or truncated pyramidal, as they related to the infratemporal crest and as they related to the pterygoid process. The presence of the infratemporal spine was found in 100 % of the studied skulls, unilaterally or bilaterally. The most common morphology was found to be laminar (40 %), followed by pyramidal (35 %), and, finally, truncated pyramidal (24 %). The majority (73 %) of these infratemporal spines was closely associated with the pterigoyd process with a complete or partial relation, with fewer (34 %) being associated with the infratemporal crest.

La espina infratemporal o tubérculo esfenoidal es un reparo óseo mencionado tanto en la literatura anatómica clásica como en los artículos más actuales. Las publicaciones disponibles no mencionan las características anatómicas ni la distribución en la población de este elemento óseo. El objetivo de este trabajo fue definir, identificar la presencia y describir la morfología de la espina infratemporal en una muestra de población chilena. Se utilizaron 57 cráneos secos de la unidad de anatomía de la Universidad de los Andes. Se evaluaron las alas mayores del hueso esfenoides tanto derechas como izquierdas en búsqueda de la espina infratemporal. Se clasificaron según sus características morfológicas, siendo piramidal, piramidal truncada o laminar, según su relación con la cresta infratemporal y según su relación con el proceso pterigoideo. Se encontró la presencia de la espina infratemporal en un 100 % de los cráneos estudiados, ya sea de manera uni o bilateral. La morfología más común correspondió a laminar con un 40 %, seguido de piramidal con un 35 % y finalmente un 24 % de morfología piramidal truncada. Se relacionaron más bien con el proceso pterigoideo ya sea de manera completa o media (73 %) y en menor medida con la cresta infratemporal (34 %).

Efecto de las variantes de VKORC1 y CYP2C9 sobre la dosis de anticoagulantes orales en individuos chilenos / Effect of VKORC1 and CYP2C9 variants on dosage of oral anticoagulants in Chilean individuals

Background: The dose of oral anticoagulants (OAC) shows great variability among patients. Pharmacogenetic studies have shown that common variants in genes CYP2C9 (*2 and *3) and VKORC1 (-1639G>A) are associated with lower requirements of OAC. Aim: To study the association between average maintenance doses of oral anticoagulant therapy required to maintain a stable INR and CYP2C9 and VKORC1 gene variants in Chilean adults. Material and Methods: Prospective study of patients on anticoagulant treatment and with a stable international normalized ratio (INR) for prothrombin time for at least three months. Patients were classified as having high or low acenocoumarol or warfarin requirements. Peripheral blood DNA genotyping was performed by polymerase chain reaction and restriction fragment polymorphism or sequencing and electrophoresis. Results: The study included 185 patients, 125 on acenocoumarol and 60 on warfarin. Patients with VKORC1-1639A allele were more likely to require lower doses of both drugs than patients with the G allele (Odds ratio [OR] for acenocoumarol 9.06, and OR for warfarin = 18.7). There was no association between CYP2C9*2 and*3 and acenocoumarol or warfarin requirements. Conclusions: There is an association between VKORC1-1639A variant and anticoagulant doses.

Morphometry of the Greater Palatal Canal in Adult Skulls.

The purpose of this study was to evaluate in dry skulls the length and angle between the greater palatine foramen and the foramen rotundum in both the frontal and sagittal planes. In 50 human skulls from the department of morphology, the distance and angulation required to reach the foramen rotundum through the greater palatine canal were measured in the frontal and sagittal planes. A stylet was introduced up to the foramen rotundum in each greater palatine canal and fixed. The skulls were then photographed from a front and lateral view (both right and left). Finally, the stylets were photographed on graph paper. These images were analyzed with Photoshop software. In the frontal plane, mean angulations of 5.32 degrees on the right side and 6.15 degrees on the left side were obtained. In the sagittal plane, mean angulations of 61.66 degrees on the right side and 61.81 degrees on the left side were obtained. Finally, the mean length required to reach the foramen rotundum was 31.95  mm on the right side and 32.49  mm on the left side. Some of these results differ from those stated in the foreign literature (10 degrees front, 70 degrees sagittal). These differences should be considered for both clinical practice and teaching in Chile.

Aplicación de técnica de hidróxido de potasio y glicerina para diafanización dentaria / Application of potassium hydroxide and glycerin technique for dental clearing

La diafanización dentaria consiste en lograr transparentar la estructura mineralizada, permitiendo observar la arquitectura interna del diente. Se realiza principalmente a partir de métodos por deshidratación de la muestra. Por otro lado existen técnicas utilizadas para el estudio de centros de osificación basadas en métodos por maceración utilizando hidróxido de potasio y glicerina, de las cuales no se tiene antecedentes de haber sido utilizada en diafanización dentaria. El objetivo de este trabajo fue describir una técnica de diafanizado por maceración de tejidos dentarios mediante hidróxido de potasio y mostrar el resultado obtenido con un seguimiento de 5 años. Se seleccionó una muestra de 40 dientes (10 incisivos, 6 caninos, 10 premolares y 14 molares). Se limpiaron los dientes (destartraje) y luego se descalcificaron parcialmente con ácido clorhídrico. El 25% de la muestra fue diafanizada con el método clásico de deshidratación. El 75% restante fue diafanizado con el método por maceración, que consistió básicamente en una maceración parcial del diente mediante baños en hidróxido de potasio de baja concentración, luego agregando glicerina en forma ascendente hasta terminar en glicerina pura por tiempo indefinido. La técnica por maceración logró diafanización adecuada de los tejidos con una óptima visualización del sistema de canales radiculares en las muestras diafanizadas. Con ambas técnicas se logró transparentar los dientes pero con diferencias evidentes. Con la primera los dientes lograron un tono amarillento, perdiéndose gran cantidad de tejido dentario en cambio las muestras diafanizadas por maceración conservaron mejor su estructura y obtuvieron un color más cercano al del diente original. Con el método por maceración se obtienen dientes diafanizados más similares a la realidad que lo logrado con el método por deshidratación.

Dental clearing objective is performed to obtain the translucency of the mineralized structure, allowing an appreciation of the inner architecture of the tooth. It is achieved mainly using the dehydration technique on the sample. On the other hand, there are other techniques used for the study of ossification centers based on the maceration methods using potassium hydroxide and glycerin, none of which have been reported for dental clearing. The purpose of this study was to describe the maceration technique of the dental tissue using potassium hydroxide with a five-year follow up. We selected 40 teeth to complete the sample (10 incisors, 6 cuspid, 10 bicuspids and 14 molars). The teeth were cleaned and then partially decalcified with hydrochloric acid. A quarter of the sample was cleared by classic dehydration method. The other three quarters were cleared with the maceration method, that consisted mainly in a partial maceration using potassium hydroxide baths at low concentrations, followed by the addition of glycerin at ascending concentrations and finally using pure glycerin for an undefined period of time. The maceration technique achieved an adequate clearing of the mineralized tissue with an optimal visualization of the root canal system in the cleared sample. With both techniques it was possibly to clear the teeth but with evident differences. With the first technique the teeth achieved a yellowish color, losing a great amount of dental tissue however the maceration technique sample allowed the preservation of more structure and obtained a coloration similar to the original tooth. With the maceration method the samples were more similar to the original ones than the cleared teeth obtained with dehydration.

Two-port laparoscopic radical cystectomy with reusable umbilical system: a feasibility study.

OBJECTIVE: To describe the technique and report outcomes of laparoscopic radical cystectomy using 2 ports (2-port LRC) for muscle-invasive bladder cancer. PATIENTS AND METHODS: Prospective study was performed between November 2011 and October 2012 to standardize 2-port LRC, lymph node dissection, and urinary diversion. Twenty patients were intervened (8 ileal conduit, 12 neobladder) and followed up for >1 yr. Median follow-up was 75.5 weeks (interquartile range, 65.2-86 weeks). A reusable system placed through the umbilicus and laparoscopic curved instruments with double rotation, plus one 10-mm extra port placed in the right iliac fossa were used. Neobladder or conduit was performed extracorporeally. Preoperative, perioperative, and pathologic outcomes and long-term security data are presented. RESULTS: Median age was 69.5 years; body mass index, 27.4 kg/m(2); operative time, 335 minutes; estimated blood loss, 337 mL; hospital stay, 9 days; intraoperative transfusion rate, 10%; and visual analog pain score, 3 at day 3. Surgical margin was positive in a case (5%); 3 (15%) were pT0, 2 (10%) pT1, 5 (25%) pT2, 6 (30%) pT3a, 3 (15%) pT3b, and 1 (5%) pT4. The number of nodes removed was 18.5 (interquartile range, 16-29.2), 4 (20%) positive. Complications were major in 2 (10%; fecal peritonitis and urinary sepsis) and minor in 4 (20%; ileus and 3 postoperative transfusion) cases. No case required additional analgesia. Incision was totally hidden in the umbilicus. Continence rate in neobladders was 91.7% at daytime and 75% at nighttime. Study limitation was the absence of a comparative cohort. CONCLUSION: Umbilical 2-port LRC is feasible with good oncologic and functional outcomes, low postoperative pain, and absence of abdominal wall complications. Difficulties have slowed laparoendoscopic single-site radical cystectomy, but umbilical 2-port LRC is a very acceptable alternative for minimally invasive surgery of muscle-invasive bladder cancer.

Vertiginous syndrome associated with incorrect anesthesia technique to block the maxillary nerve via the greater palatine canal: case report and anatomic correlation with cadaveric parts / Síndrome vertiginoso asociado a técnica anestésica incorrecta para bloquear el nervio maxilar vía canal palatino mayor: reporte de caso y correlación anatómica en piezas cadavéricas

The anesthetic technique through the greater palatine canal seeks to block the maxillary nerve in the pterygopalatine fossa and anesthetize a large area, including the pulp and periodontium of the arch in question. After applying this technique in a patient, it failed to obtain the expected result. The patient began to experience dizziness, nausea, vomiting and the sensation of fluid in the ear. She was evaluated in both the emergency room of the Hospital Parroquial de San Bernardo and at a private clinic without accurate diagnosis. Only symptomatic treatment was provided. The next day she was discharged with reduced symptoms, which disappeared completely during the day. We propose the hypothesis of a diffusion of the anesthetic solution into the middle and inner ear through the auditory tube. This diffusion would explain the vestibular symptoms and the absence of anesthesia in the expected areas. We carried out an anatomic correlation in cadavers, following the path of a needle from the palatal mucosa to the pharyngeal opening of auditory tube. We conclude that the vertiginous syndrome could be due to an incorrect application of the technique, with the needle entering the auditory tube and spreading the anesthetic solution into the middle ear.

La técnica anestésica vía canal palatino mayor tiene como objetivo abordar al nervio maxilar en la fosa pterigopalatina, anestesiando un gran territorio, incluyendo la pulpa y periodonto de la hemiarcada correspondiente. Después de haber aplicado esta técnica en una paciente y no obteniendo el resultado esperado, esta comenzó a experimentar vértigo, náuseas, sensación de líquido en el oído y vómitos. Fue evaluada en el servicio de urgencias del Hospital Parroquial de San Bernardo y en una Clínica Privada, sin lograr un diagnóstico preciso y realizando solo un tratamiento sintomático. Al día siguiente fue dada de alta con baja sintomatología, la cual desapareció totalmente durante el día. Se propone la hipótesis de una difusión del anestésico hacia el oído medio e interno mediante el tubo auditivo. Esto explicaría por un lado la sintomatología vestibular y por otro la ausencia de anestesia en los dientes y territorios esperados. Además se realizó una correlación anatómica en cadáveres, utilizando 8 hemicabezas conservadas y siguiendo el posible trayecto de la aguja desde la mucosa palatina hasta el orificio faríngeo de la tuba auditiva. Se concluyó que el síndrome vertiginoso experimentado por la paciente se pudo deber a una técnica fallida al nervio maxilar vía canal palatino mayor con ingreso de la aguja al tubo auditivo, difundiendo el anestésico hacia el oído medio.

Influencia de dientes remanentes en la presencia y morfometría de forámenes y canales en relación a las espinas mentales / Influence of remnant teeth in the presence and morphometry of foramina and canals in relation to mental spine

La zona anterior de la mandíbula, con frecuencia es intervenida desde el punto de vista quirúrgico (instalación de implantes oseointegrados, mentoplastias, genioplastias, profundizaciones de vestíbulo y flanco lingual, fracturas, etc.). Se ha descrito la presencia de vasos que penetran la tabla ósea lingual en la zona mediana mandibular, los cuales se relacionan con forámenes vasculares. La pérdida dentaria en la zona anterior mandibular podría influir en el tamaño de los forámenes y canales debido a la degeneración de los vasos incisivos provenientes de la arteria alveolar inferior. El objetivo fue evaluar la relación entre presencia y ausencia de dientes remanentes en el grupo V con la presencia y morfometría de forámenes y canales ubicados en cercanía a las espinas mentales. Se estudiaron 95 mandíbulas humanas secas, con sexo y edad indeterminada. Se midió el área de los forámenes y la longitud de los canales utilizando el programa Photoshop CS3 extended. Se obtuvo un total de 53 mandíbulas dentadas en el grupo V y 42 mandíbulas desdentadas en el grupo V. El 95,7% de la muestra tuvo al menos un foramen en relación a las espinas mentales. Se vio que los forámenes con mayor frecuencia de aparición fueron los superiores a las espinas mentales superiores en 75 mandíbulas, forámenes inferiores a las espinas mentales inferiores en 72 mandíbulas y los forámenes entre las espinas mentales en 26 mandíbulas. No se encontró asociación entre la presencia y ausencia de dientes con la frecuencia de forámenes en las tres posiciones estudiadas. Se observó que el foramen inferior a espinas mentales tuvo un diámetro mayor en mandíbulas dentadas (valor p= 0,0194) y la longitud del canal superior a las espinas mentales superiores fue significativamente más largo en mandíbulas desdentadas (valor p= 0,0206).

The anterior mandibular body is an anatomical area that is often subjected to surgery such as for installation of osseointegrated implants, mentoplasty, genioplasty, fractures, among others. The presence of vessels that penetrates through foraminas at its midline from the lingual table has been described. Tooth loss in the anterior mandibular zone may influence the size of the foramina and canals due to the atrophy of incisors vessels that originates from the inferior alveolar artery. The aim was to evaluate the relationship between presence and absence of remaining teeth in group V, with the presence and morphometry of foramina and canals located in proximity to the mental spines. We studied 95 dry human mandibles with indeterminate sex and age. We measured the area of the foraminas and the length of the canals using the program Photoshop CS3 Extended. We obtained a total of 53 dentate and 42 edentulous mandibles in group V. In 95.7% of the sample had the presence of at least one foramina relative to the mental spines. The foramina that was found more often, tended to be upper to the superior mental spines, in a total of 75 mandibles; lower to the inferior mental spines in 72 mandibles and between the mental spines in 26 mandibles. There was no association between the presence and absence of teeth versus the frequency of the foramina in the three positions established. It was seen that the foramina lower to the inferior mental spine had a greater diameter in dentate mandibles (p-value= 0.0194) and the canal upper to the superior mental spines was significantly longer in the edentulous mandibles (p-value= 0.079).

Umbilical laparoendoscopic urological surgery with a novel reusable device.

OBJECTIVE: The aims of this study were to present cumulative experience with umbilical laparoendoscopic urological surgery using a reusable device and to evaluate outcomes and complications in the first 100 patients. MATERIAL AND METHODS: Patients undergoing umbilical surgery with the KeyPort system and DuoRotate instruments (Richard Wolf, Knittlingen, Germany) were evaluated prospectively. Demographic, intraoperative and postoperative data were assessed. RESULTS: Between October 2011 and July 2012, 79 pelvic (66 radical prostatectomy, 10 radical cystectomy, one diverticulectomy, one bilateral orchiectomy, one ureter reimplantation) and 21 renal (seven radical nephrectomy, six partial nephrectomy, five nephroureterectomy, two pyeloplasty, one pyelolithotomy) surgeries were performed through the umbilicus using this platform. Follow-up was 56.7 ± 12.6 weeks (mean ± SD). Mean age was 64.3 ± 10.3 years, body mass index 29 ± 4.6 kg/m(2), operative time 232 ± 106 min and estimated blood loss 260 ± 95 ml. Conversion to standard multiport laparoscopy was not necessary. An accessory port was used in 87 cases to facilitate suturing and conduct drainage extraction. Postoperative complications occurred in 24 cases (six Clavien grade I, 12 grade II, one grade IIIa, two grade IIIb, two grade IVa, one grade IVb). Mean hospital stay was 4.2 ± 4 days. Total transfusion rate was 10%. Mean visual analogue pain scale at day 2 was 2.1 ± 1.3 (0-10). Visual analogue wound satisfaction scale at month 1 was 9.2 ± 0.6 (0-10). No cancer-related events occurred during follow-up. Late complications (4%) were not related to the surgical approach. CONCLUSIONS: Umbilical KeyPort surgery is technically feasible for a great variety of procedures, both ablative and reconstructive. This access offers adequate surgical outcomes, scarce postoperative pain and security for the patient in the short term. Its reusable nature implies a noticeable economic advantage.

Initial experience of umbilical laparoendoscopic single-site nephron-sparing surgery with KeyPort and DuoRotate system.

OBJECTIVES: To present the feasibility of laparoendoscopic single-site (LESS) nephron-sparing surgery (NSS) using new reusable umbilical single-port system and instruments. METHODS: A prospective study was performed to evaluate operative data and patient outcomes after LESS-NSS with KeyPort®, a tri-channel single-port placed through a 2.5-cm umbilical incision, and specific DuoRotate® instruments. Patient characteristics, operative time, estimated blood loss (EBL), complications, visual analog pain scale (VAPS), and visual analogue wound satisfaction scale (VAWSS) scores were registered. RESULTS: Six consecutive nonselected patients with indication of NSS and normal contralateral kidney were offered LESS-NSS. An accessory 3.5 mm port that facilitated renoraphy and unclamped technique was used in 4 (66.7%) cases each. Median tumor maximum size was 4.0 (1-7.5) cm; age 64 (31-76) years; body mass index 28.4 (22.1-39.3) kg/m(2); operative time 233.5 (140-250) min; EBL 500 (200-500) mL; difference in hemoglobin 2.3 (0.1-4.6) g/dL. VAPS at day 2 was 0.7/10 and the median length of stay 3 (2-4) days. One case (16.7%) needed transfusion. None required conversion to standard laparoscopy or use of other additional ports. Postoperative complications occurred in 3 (50%) and major complications in 1 (16.7%). Incisions were totally hidden in the umbilicus. Pathology revealed angiomyolipoma (3), renal cell carcinoma (2), and chronic inflammation (1). Tumor margins were negative in all cases with malignancy. VAWSS at first month was 9.4 (8.1-10). At a median follow-up of 24 (10-32) weeks, no patient developed complications related to the approach. CONCLUSIONS: Umbilical LESS-NSS through a new single-port system of reusable nature, with or without the help of an accessory port and occasionally without clamping, can be effectively and very economically performed with minimum postoperative pain. Good candidates are patients with presumed benign renal masses that appreciate the cosmetic advantage of the approach.

Umbilical KeyPort bilateral laparoscopic orchiectomy in patient with complete androgen insensitivity syndrome.

MAIN FINDINGS: A 22-year-old woman with complete androgen insensitivity syndrome (CAIS) presenting with primary amenorrhea and normal female external genitalia was referred for laparoscopic gonadectomy. She had been diagnosed several years earlier but was reluctant to undergo surgery. Case HYPOTHESIS: Diagnosis of this X-linked recessive inherited syndrome characterizes by disturbance of virilization in males with an AR mutation, XY karyotipe, female genitalia and severely undescended testis with risk of malignization. The optimal time to orchidectomy is not settled; neither the real risk of malignancy in these patients. Early surgery impacts development of a complete female phenotype, with enlargement of the breasts. Based on modern diagnostic imaging using DCE-MRI and surgical technology with single port laparoscopic access we hypothesize that the optimum time for gonadectomy is not at the time of diagnosis, but once feminization has completed. PROMISING FUTURE IMPLICATIONS: An umbilical laparoendoscopic single-site access for bilateral gonadectomy appears to be the first choice approach as leaves no visible incision and diminishes the psychological impact of surgery in a patient with CAIS absolutely reassured as female. KeyPort, a single port access with duo-rotate instruments developed by Richard Wolf facilitates this surgery and allows excellent cosmetic results.

Umbilical KeyPort bilateral laparoscopic orchiectomy in patient with complete androgen insensitivity syndrome

MAIN FINDINGS: A 22-year-old woman with complete androgen insensitivity syndrome (CAIS) presenting with primary amenorrhea and normal female external genitalia was referred for laparoscopic gonadectomy. She had been diagnosed several years earlier but was reluctant to undergo surgery. CASE HYPOTHESIS: Diagnosis of this X-linked recessive inherited syndrome characterizes by disturbance of virilization in males with an AR mutation, XY karyotipe, female genitalia and severely undescended testis with risk of malignization. The optimal time to orchidectomy is not settled; neither the real risk of malignancy in these patients. Early surgery impacts development of a complete female phenotype, with enlargement of the breasts. Based on modern diagnostic imaging using DCE-MRI and surgical technology with single port laparoscopic access we hypothesize that the optimum time for gonadectomy is not at the time of diagnosis, but once feminization has completed. PROMISING FUTURE IMPLICATIONS: An umbilical laparoendoscopic single-site access for bilateral gonadectomy appears to be the first choice approach as leaves no visible incision and diminishes the psychological impact of surgery in a patient with CAIS absolutely reassured as female. KeyPort, a single port access with duo-rotate instruments developed by Richard Wolf facilitates this surgery and allows excellent cosmetic results.

Presencia y morfometría de forámenes y canales en relación a las espinas mentonianas / Presence and morphometry of foramina and canals in relation to mental spines

Los procedimientos quirúrgicos desarrollados en la porción anterior del cuerpo mandibular, como por ejemplo la instalación de implantes oseointegrados, genioplastias, profundizaciones de flanco lingual, fracturas, etc. pueden presentar complicaciones de importancia cuando estas invaden estructuras vasculares, con la consecuente hemorragia y formación de hematomas sublinguales. Dentro de estos elementos anatómicos se encuentran forámenes y canales que se ubican en relación a las espinas mentonianas. El objetivo de este estudio fue cuantificar la ubicación, diámetro y longitud de los forámenes y canales presentes por superior, inferior y entre las espinas mentonianas superiores e inferiores en 71 mandíbulas secas de la Unidad de Morfología de la Universidad de los Andes las que fueron fotografiadas. Analizando las fotografías con el programa Photoshop® Cs 3 extended, se midieron en pixeles el diámetro y longitud de los forámenes y canales para luego transformarlos en mm. El 97 por ciento de las mandíbulas observadas presentaron al menos un foramen, el 45 por ciento presentó dos y el 31 por ciento tres. En relación a su ubicación el 82 por ciento de las mandíbulas presentó forámenes por superior a las espinas y el 76 por ciento por inferior. El diámetro promedio fue de 0,73 mm para los de ubicación superior y de 0,71 mm para los de ubicación inferior. La longitud promedio de los canales fue de 6,77 mm para los ubicados por superior y de 5,5 mm para los de ubicación inferior a las espinas mentonianas. Debido a la alta prevalencia de forámenes y canales en nuestra muestra es recomendable tenerlos siempre en cuenta en la planificación de intervenciones quirúrgicas que involucren ya sea el flanco lingual mediano mandibular o el cuerpo mandibular en la zona de la sínfisis.

Surgical procedures developed in the anterior mandibular body, such as the installation of osseointegrated implants, genioplasties, lingual edge insights, fractures, etc. may produce mayor complications when these invade vascular structures, with consequent bleeding and sublingual hematoma formation. Within these anatomical elements we find foramina and canals that are located in relation to the mental spine. The objective of this study was to quantify the location, diameter and length of the foramina and canals present superior, inferior and between the upper and lower mental spine in 71 dry mandibles from the Morphology Unit, Universidad de los Andes, which were photographed. Analyzing the pictures with Photoshop ® Cs 3 extended program, the diameter and length of the foramina and canals were measured in pixels and then transformed into mm. 97 percent of the jaws observed had at least one foramen, 45 percent had two and 31 percent three. In relation to location 82 percent of the mandibles showed foramina in the bones superior and inferior 76 percent. The average diameter was 0.73 mm for superior placement and 0.71 mm for inferior placement. The average length of the superior canals was 6.77 mm and 5.5 mm for those inferior to the mental spine. Due to the high prevalence of foramina and canals in our sample its recommended to keep always in mind when planning surgical procedures that involve either medium mandibular lingual flank as the mandibular body at the symphysis area.

Safety study of umbilical single-port laparoscopic radical prostatectomy with a new DuoRotate system.

BACKGROUND: Laparoendoscopic single-site (LESS) radical prostatectomy (RP) has been performed through different approaches. A new DuoRotate manual system developed by Richard Wolf (KeyPort; Richard Wolf GmbH, Knittlingen, Germany) can be applied to RP. OBJECTIVES: Our aim was to describe the surgical technique and report early outcomes of KeyPort LESS-RP to determine if this procedure is feasible and safe. DESIGN, SETTING, AND PARTICIPANTS: Prospective study performed between October 2011 and January 2012 to standardize LESS-RP. A total of 31 procedures were performed (10 with and 21 without neurovascular preservation, 8 with and 23 without pelvic lymph node dissection). SURGICAL PROCEDURE: LESS-RP was performed using the methods outlined in the manuscript. All patients underwent LESS RP by the same surgical team. Access was achieved via a tri-channel reusable KeyPort and one 3.5-mm extra port to facilitate urethrovesical anastomosis and drainage extraction. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Preoperative, perioperative, and pathologic outcomes data are presented. RESULTS AND LIMITATIONS: The mean age of the patients was 64 yr; mean body mass index: 30.7 kg/m(2); mean prostate-specific antigen level: 7 ng/ml; mean operative time: 207 min; and mean estimated blood loss: 258 ml. The average length of stay was 2.9 d and visual analog pain score (range: 0 [no pain] to 10) at day 2 was 1.2. Five focal positive margins (16.7%) were encountered (4.4% for pT2 and 57.1% for pT3). Five cases (16.7%) were pT2a, 3 (10%) were pT2b, 15 (50%) were pT2c, and 7 (23.3%) were pT3a. Lymph node dissection results were negative in all patients. Major complications occurred in two patients (6.5%) (hypercapnia with respiratory acidosis and rectourethral fistula) and minor complications in four (12.9%) (atrial fibrillation, orchitis, transfusion, and vomiting). No case required additional analgesia. Incision was totally hidden in the umbilicus. Study limitations included short follow-up (mean: 20.2 ± 4.1 wk), premature functional data, and absence of a comparative cohort. CONCLUSIONS: The KeyPort system allows performance of umbilical RP with few complications, a low positive-margin rate, excellent aesthetic results, and very low postoperative pain levels.