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1.
Environ Sci Pollut Res Int ; 31(5): 7543-7555, 2024 Jan.
Artículo en Inglés | MEDLINE | ID: mdl-38165545

RESUMEN

The elimination of antimony pollution has attracted increasing concerns because of its high toxicity to human health and the natural environment. In this work, biomimetic δ-MnO2 was synthesized by using waste tobacco stem-silks as biotemplate (Bio-δ-MnO2) and used in the capture of Sb(III)from aqueous solution. The tobacco stem-silks not only provided unique wrinkled morphologies but also contained carbon element self-doped into the resulting samples. The maximum Sb(III) adsorption capacity reached 763.4 mg∙g -1, which is 2.06 times higher than δ-MnO2 without template (370.0 mg∙g -1), 4.53 times than tobacco stem-silks carbon (168.5 mg∙g -1), and 10.39 times than commercial MnO2 (73.5 mg∙g -1), respectively. The isotherm and kinetic studies indicated that the adsorption behavior was consistent with the Langmuir isotherm model and the pseudo-second-order kinetic equation. As far as we are aware, the adsorption capacity of Bio-δ-MnO2 is much higher than that of most Sb(III) adsorbents. FT-IR, XPS, SEM, XRD, and Zeta potential analyses showed that the main mechanism for the adsorption of Sb(III) by Bio-δ-MnO2 includes electrostatic attraction, surface complexation, and redox. Overall, this study provides a new sustainable way to convert agricultural wastes to more valuable products such as biomimetic adsorbent for Sb(III) removal in addition to conventional activated carbon and biochar.


Asunto(s)
Óxidos , Contaminantes Químicos del Agua , Humanos , Cinética , Compuestos de Manganeso , Espectroscopía Infrarroja por Transformada de Fourier , Contaminantes Químicos del Agua/análisis , Adsorción
2.
Molecules ; 28(1)2023 Jan 02.
Artículo en Inglés | MEDLINE | ID: mdl-36615581

RESUMEN

In this study, Co-doped TiO2 was synthesized using waste tobacco stem silk (TSS) as a template via a one-pot impregnation method. These samples were characterized using various physicochemical techniques such as N2 adsorption/desorption analysis, diffuse reflectance UV-visible spectroscopy, X-ray diffraction, field-emission scanning electron microscopy, high-resolution transmission electron microscopy, X-ray photoelectron spectroscopy, photoluminescence spectroscopy, and electron paramagnetic resonance spectroscopy. The synthesized material was used for the photodegradation of tetracycline hydrochloride (TCH) under visible light (420-800 nm). No strong photodegradation activity was observed for mesoporous TiO2 synthesized using waste TSS as a template, mesoporous Co-doped TiO2, or TiO2. In contrast, Co-doped mesoporous TiO2 synthesized using waste TSS as a template exhibited significant photocatalytic degradation, with 86% removal of TCH. Moreover, owing to the unique chemical structure of Ti-O-Co, the energy gap of TiO2 decreased. The edge of the absorption band was redshifted, such that the photoexcitation energy for generating electron-hole pairs decreased. The electron-hole separation efficiency improved, rendering the microstructured biotemplated TiO2 a much more efficient catalyst for the visible-light degradation of TCH.


Asunto(s)
Nicotiana , Tetraciclina , Luz , Antibacterianos/química , Titanio/química , Catálisis
3.
Angew Chem Int Ed Engl ; 61(38): e202208721, 2022 09 19.
Artículo en Inglés | MEDLINE | ID: mdl-35906932

RESUMEN

A series of dinuclear RuII complexes with extremely high TPA cross sections in the range of 800-900 nm have been designed. The amphiphilic complex Ru3 containing tert-butyl groups has balanced performance in singlet oxygen generation and photothermal conversion and becomes the ideal drug candidate of the series. Ru3 targets mitochondria without penetrating the nucleus, which substantially increases its photodynamic therapy activity and reduces its dark cytotoxicity. Ru3 successfully suppresses melanoma tumor growth in vitro and in vivo with combined photodynamic and photothermal therapy under low light dose irradiation of an 808 nm low-power laser, avoiding the known PDT resistance in melanoma. The excellent therapeutic effect of Ru3 facilitates its applications in further human trials for larger or deeper buried tumors, thereby becoming a prospective candidate for a new generation of low-power IR-driven dual PDT/PTT drugs.


Asunto(s)
Melanoma , Fotoquimioterapia , Rutenio , Línea Celular Tumoral , Humanos , Rayos Láser , Melanoma/tratamiento farmacológico , Mitocondrias , Fármacos Fotosensibilizantes/farmacología , Fármacos Fotosensibilizantes/uso terapéutico , Terapia Fototérmica , Rutenio/farmacología
4.
Biomater Biosyst ; 5: 100038, 2022 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-36825110

RESUMEN

Protease has been widely used in biological and industrial fields. Developing efficient artificial enzyme mimics remains a major technical challenge due to the high stability of peptide bonds. Nanoenzymes with high stability, high activity and low cost, provided new opportunities to break through natural enzyme inherent limitations. However, compared with many nanomaterials with inherent peroxidase activity, the intrinsic mimic proteases properties of magnetic nanomaterials were seldom explored, let alone the interaction between magnetic nanomaterials and cellular proteins. Herein, we reported for the first time that magnetic CuFe2O4 possesses inherent protease activity to hydrolyze bovine serum albumin (BSA) and casein under physiological conditions, and the CuFe2O4 is more resistant to high temperature than the natural trypsin. It also exhibited significantly higher catalytic efficiency than other copper nanomaterials and can be recycled for many times. Protease participated in pathophysiological processes and all stages of tumor progression. Interesting, CuFe2O4 exhibited anti-proliferative effect on A549, SKOV3, HT-29, BABL-3T3 and HUVEC cells, as well as it was particularly sensitive against SKOV3 cells. CuFe2O4 was about 30 times more effective than conventional chemotherapy drugs oxaliplatin and artesunate against SKOV3 cells. In addition, CuFe2O4 also mediated the expression of intracellular proteins, such as MMP-2, MMP-9, F-actin, and NF-kB, which may be associated with global protein hydrolysis by CuFe2O4, leading to inhibition of cell migration. The merits of the high magnetic properties, good protease-mimic and antitumor activities make CuFe2O4 nanoparticles very prospective candidates for many applications such as proteomics and biotechnology.

5.
J Nanobiotechnology ; 19(1): 68, 2021 Mar 04.
Artículo en Inglés | MEDLINE | ID: mdl-33663548

RESUMEN

It was shown that some nanomaterials may have anticancer properties, but lack of selectivity is one of challenges, let alone selective suppression of cancer growth by regulating the cellular microenvironment. Herein, we demonstrated for the first time that carbon quantum dots/Cu2O composite (CQDs/Cu2O) selectively inhibited ovarian cancer SKOV3 cells by targeting cellular microenvironment, such as matrix metalloproteinases, angiogenic cytokines and cytoskeleton. The result was showed CQDs/Cu2O possessed anticancer properties against SKOV3 cells with IC50 = 0.85 µg mL-1, which was approximately threefold lower than other tested cancer cells and approximately 12-fold lower than normal cells. Compared with popular anticancer drugs, the IC50 of CQDs/Cu2O was approximately 114-fold and 75-fold lower than the IC50 of commercial artesunate (ART) and oxaliplatin (OXA). Furthermore, CQDs/Cu2O possessed the ability to decrease the expression of MMP-2/9 and induced alterations in the cytoskeleton of SKOV3 cells by disruption of F-actin. It also exhibited stronger antiangiogenic effects than commercial antiangiogenic inhibitor (SU5416) through down-regulating the expression of VEGFR2. In addition, CQDs/Cu2O has a vital function on transcriptional regulation of multiple genes in SKOV3 cells, where 495 genes were up-regulated and 756 genes were down-regulated. It is worth noting that CQDs/Cu2O also regulated angiogenesis-related genes in SKOV3 cells, such as Maspin and TSP1 gene, to suppress angiogenesis. Therefore, CQDs/Cu2O selectively mediated of ovarian cancer SKOV3 cells death mainly through decreasing the expression of MMP-2, MMP-9, F-actin, and VEGFR2, meanwhile CQDs/Cu2O caused apoptosis of SKOV3 via S phase cell cycle arrest. These findings reveal a new application for the use of CQDs/Cu2O composite as potential therapeutic interventions in ovarian cancer SKOV3 cells.


Asunto(s)
Carbono/farmacología , Muerte Celular/efectos de los fármacos , Citocinas/metabolismo , Citoesqueleto/metabolismo , Metaloproteinasas de la Matriz/metabolismo , Nanocompuestos/química , Neoplasias Ováricas/tratamiento farmacológico , Puntos Cuánticos/química , Inductores de la Angiogénesis , Inhibidores de la Angiogénesis/química , Inhibidores de la Angiogénesis/farmacología , Animales , Antineoplásicos/química , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Ciclo Celular , Puntos de Control del Ciclo Celular/efectos de los fármacos , Línea Celular Tumoral , Cobre/química , Cobre/farmacología , Regulación hacia Abajo/efectos de los fármacos , Femenino , Humanos , Metaloproteinasa 9 de la Matriz/metabolismo , Ratones , Receptor 2 de Factores de Crecimiento Endotelial Vascular/metabolismo
6.
Inorg Chem ; 59(20): 14920-14931, 2020 Oct 19.
Artículo en Inglés | MEDLINE | ID: mdl-32951429

RESUMEN

Photodynamic therapy (PDT) using two-photon near-infrared light excitation is a very effective way to avoid the use of short-wavelength ultraviolet or visible light which cannot efficiently penetrate into the biological tissues and is harmful to the healthy cells. Herein, a series of cyclometalated Ir(III) complexes with a structurally simple diimine ligand were designed and the synthetic route and preparation procedure were optimized, so that the complexes could be obtained in apparently higher yield, productivity, and efficiency in comparison to the traditional methods. Their ground state and excited singlet and triplet state properties were studied by spectroscopy and quantum chemistry theoretical calculations to investigate the effect of substituent groups on the photophysical properties of the complexes. The Ir(III) complexes, especially Ir1 and Ir3, showed very low dark toxicities and high phototoxicities under both one-photon and two-photon excitation, indicating their great potential as PDT agents. They were also found to be highly sensitive two-photon mitochondria dyes.


Asunto(s)
Antineoplásicos/farmacología , Complejos de Coordinación/farmacología , Colorantes Fluorescentes/farmacología , Mitocondrias/metabolismo , Fármacos Fotosensibilizantes/farmacología , Células A549 , Antineoplásicos/síntesis química , Antineoplásicos/efectos de la radiación , Complejos de Coordinación/síntesis química , Complejos de Coordinación/efectos de la radiación , Colorantes Fluorescentes/síntesis química , Colorantes Fluorescentes/efectos de la radiación , Humanos , Iridio/química , Iridio/efectos de la radiación , Fotones , Fármacos Fotosensibilizantes/síntesis química , Fármacos Fotosensibilizantes/efectos de la radiación , Oxígeno Singlete/metabolismo
7.
ACS Appl Bio Mater ; 3(7): 4081-4094, 2020 Jul 20.
Artículo en Inglés | MEDLINE | ID: mdl-35025483

RESUMEN

Metals-organic frameworks (MOFs) have been widely explored in biomedicine, mostly in drug delivery, biosensing, and bioimaging due to their large surface area, tunable porosity, readily chemical functionalization, and good biocompatibility. However, the underlining cellular mechanisms controlling the process for MOF cytotoxicity remains almost completely unknown. Here, we demonstrate that pristine Cu-MOF without any loaded drug selectively inhibited ovarian cancer mainly through promoting tubulin polymerization and destroying the cell actin cytoskeleton (F-actin) to trigger the mitotic catastrophe, accompanying by conventional programmed cell death. To our knowledge, this is the first report claiming that mitotic catastrophe may be an explaining mechanism of MOF cytotoxicity. Cu-MOF with an intrinsic protease-like activity also hydrolyzed cellular cytoskeleton proteins (F-actin). The RNA sequencing data indicated the differential expressional mRNA of cell proliferation and actin cytoskeleton (ACTA2, ACTN3, FSCN2, and SCIN) and mitotic spindles (PLK1 and TPX2) related genes. We found that Cu-MOF as a promising candidate in the disruption of cellular cytoskeleton and the change of the gene expression could be actin altering and antimitotic agents against cancer cells, allowing for fundamental biological and biophysical studies of MOFs.

8.
Sci Rep ; 6: 26126, 2016 05 18.
Artículo en Inglés | MEDLINE | ID: mdl-27188337

RESUMEN

Though metal-organic frameworks (MOFs) have inspired potential applications in biomedicine, cytotoxicity studies of MOFs have been relatively rare. Here we demonstrate for the first time that an easily available MOF, Fe-MIL-101, possesses intrinsic activity against human SKOV3 ovarian cancer cells and suppress the proliferation of SKOV3 cells (IC50 = 23.6 µg mL(-1)) and normal mouse embryonic fibroblasts (BABL-3T3, IC50 = 78.3 µg mL(-1)) cells. It was more effective against SKOV3 cells than typical anticancer drugs such as artesunate (ART, IC50 = 96.9 µg mL(-1)) and oxaliplatin (OXA, IC50 = 64.4 µg mL(-1)), but had less effect on normal BABL-3T3 cells compared with ART (IC50 = 36.6 µg mL(-1)) and OXA (IC50 = 13.8 µg mL(-1)). Fe-MIL-101 induced apoptosis of human umbilical vein endothelial cells (HUVECs) via G0/G1 cell cycle arrest and decreased the mitochondrial membrane potential in HUVECs and induced apoptosis. Furthermore, Fe-MIL-101 exhibited stronger antiangiogenic effects in HUVEC cells than antiangiogenic inhibitor (SU5416) via downregulation the expression of MMP-2/9. Our results reveal a new role of Fe-MIL-101 as a novel, non-toxic anti-angiogenic agent that restricted ovarian tumour growth. These findings could open a new avenue of using MOFs as potential therapeutics in angiogenesis-dependent diseases, including ovarian cancer.


Asunto(s)
Antineoplásicos/metabolismo , Proliferación Celular/efectos de los fármacos , Complejos de Coordinación/metabolismo , Hierro/metabolismo , Inhibidores de la Metaloproteinasa de la Matriz/metabolismo , Neovascularización Fisiológica/efectos de los fármacos , Animales , Línea Celular , Femenino , Humanos , Concentración 50 Inhibidora , Estructuras Metalorgánicas , Ratones
9.
Oncol Lett ; 11(3): 1693-1698, 2016 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-26998063

RESUMEN

While the anti-tumor actions of ginsenosides from Panax notoginseng are well-studied, the anti-proliferative activity of 20(S)-protopanaxadiol saponins (PDS) in Sanchi ginseng on human ovarian cancer has not been reported, nor has its effect on migration of SKOV3 cells been investigated. In the present study, a wound-healing assay indicated that PDS inhibited the migration of SKOV3 cells, and a Matrigel™ tube formation assay demonstrated the presence of inhibitory tube-structures following treatment with PDS. To date, there are no previous reports on the regulation of osteopontin (OPN), a glycophosphoprotein cytokine frequently expressed in ovarian carcinoma effusions by PDS. A reduction in the expression of OPN following PDS-treatment was observed using immunohistochemical and western blot experiments. These results suggest that PDS may be useful in the search for a potential ovarian cancer treatment.

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