Correlation Between Proinflammatory Cytokine Expression and Clinical Data in Apical Granuloma.

INTRODUCTION: This study was intended to evaluate the expression of inflammatory cytokines commonly secreted by CD4+ T cells (IL-2, IL-5, IL-17, TGF-ß, TNF-α, and IFN-γ) in apical granulomas and correlate with the clinical conditions and time elapsed since root canal treatment. METHODS: Eighteen biopsy specimens obtained by periradicular surgery of teeth with post-treatment apical periodontitis and diagnosed as apical granuloma were available from the oral pathology laboratory. Silanized slides containing paraffin sections were used for immunohistochemical reactions. Images were analyzed by using an optical microscopy and each slide was subdivided into 5 fields at high magnification. RESULTS: IFN-γ and TGF-ß were the cytokines with the highest expression levels. There were statistically significant differences when comparing IL-2 and IFN-γ (P < .05), and IL-2 and TGF-ß (P < .05). Comparison between the detected cytokines and clinical data and time of treatment demonstrated significant correlation (P < .05) between lower expression of IL-2 and the presence of painful symptoms, absence of sinus tract, and treatments performed more than 4 years before. It was also possible to observe a significant correlation between lower expression of IL-5 and treatments performed less than 4 years before (P < .05). CONCLUSION: IFN-γ and TGF-ß were highly expressed in apical granulomas. However, only IL-2 and IL-5 levels were associated with clinical data and time since previous root canal treatment.

Pro-inflammatory and anti-inflammatory cytokine expression in post-treatment apical periodontitis.

OBJECTIVE: This study evaluated the expression of pro-inflammatory (IL-1ß, IL-6, IFN-γ and TNF-α) and anti-inflammatory (IL-4 and TGF-ß) cytokines in apical periodontitis lesions. Correlations between these cytokines and clinical and cone-beam computed tomographic (CBCT) data were also assessed. MATERIAL AND METHODS: Apical periodontitis lesions' data were obtained from 27 patients subjected to periradicular surgery. Specimens were processed for histopathologic and immunohistochemical analysis. Sections were evaluated according to the amount of positive staining for each antibody. Expression levels of the target mediators were compared with clinical and CBCT data. RESULTS: Twenty lesions were diagnosed as granuloma and 7 as cyst. In granulomas, IL-4 expression was significantly higher than IL-6 (p=0.001) and TNF-α (p=0.001). There was a significant relationship between high levels of TNF-α and lesions <5 mm (p=0.017). In cysts, IL-6 expression was significant lower than IL-4 (p=0.001) and IFN-γ (p=0.004). There was a significant relationship between high levels of TGF-ß and endodontic treatment performed ≤4 years before (p=0.045). In general, IL-4 was the most expressed mediator in both cysts and granulomas. CONCLUSIONS: There was a balance between the expression of pro-inflammatory and anti-inflammatory cytokines associated with the chronic periradicular inflammatory process. TNF-α and TGF-ß were related to some clinical and CBCT data.

Os cistos radiculares poem curar após tratamento endodôntico / Radicular cysts may heal after endodontic treatment

A etiologia das lesões perirradiculares e suas diferentes manifestações clínicas já estão bastante esclarecidas, porém o tratamento dos cistos radiculares ainda é um assunto controverso na Odontologia. O objetivo deste trabalho é realizar uma análise crítica, baseada na literatura, sobre o tratamento de cistos radiculares, buscando evidências que demonstrem que os cistos radiculares podem ser eliminados após tratamento endodôntico. Os cistos podem ser divididos em: cistos em bolsa e cistos verdadeiros, sendo que os cistos em bolsa respondem ao tratamento endodôntico, enquanto os cistos verdadeiros somente podem ser tratados através da cirurgia perirradicular. Pode-se concluir que sendo o agente microbiano o responsável pelas lesões perirradiculares, a maioria destas lesões, incluindo os cistos, regridem após a intervenção endodôntica não cirúrgica.

The etiology of apical periodontitis and its different clinical manifestations are already well versed, but the treatment of radicular cysts is still a controversial subject in Dentistry. The e aim of this study is to perform a critical analysis based on literature regarding treatment of radicular cysts, seeking evidence demonstrating that the radicular cysts can be eliminated after endodontic treatment. The cysts can be divided in: Apical Pocket Cysts and true cysts. Apical pocket cysts respond to endodontic treatment, while true cysts can only be treated by periradicular surgery. It can be concluded that since the microbial agent is responsible for the apical periodontitis, the majority of these lesions, including cysts regress after nonsurgical endodontic therapy.