Asunto(s)
Incompatibilidad de Grupos Sanguíneos/diagnóstico , Errores Médicos/prevención & control , Reacción a la Transfusión , Sistema del Grupo Sanguíneo ABO , Incompatibilidad de Grupos Sanguíneos/etiología , Transfusión Sanguínea/estadística & datos numéricos , Humanos , Errores Médicos/estadística & datos numéricos , Sistema del Grupo Sanguíneo Rh-Hr , Federación de RusiaAsunto(s)
Donantes de Sangre , Tipificación y Pruebas Cruzadas Sanguíneas , Trasplante de Médula Ósea , Prueba de Histocompatibilidad , Donantes de Tejidos , Tipificación y Pruebas Cruzadas Sanguíneas/tendencias , Médula Ósea/inmunología , Antígenos HLA/análisis , Prueba de Histocompatibilidad/tendencias , Humanos , Reacción a la Transfusión , Inmunología del Trasplante , U.R.S.S.RESUMEN
The human blast cells line L-101 isolated from blood leukocytes of the patient with the cutaneous erythromyeloleukemia in the media with dimethyl-sulfoxide (DMSO) and maintained by permanent cultivation stimulate myeloid differentiation of this cells. The number of differentiated cells depends on DMSO concentration. Maximum of differentiated cells (up to 75%) have exposed on the 6th day of incubation with 0,75% DMSO. The cell line L-101 is a suitable model for investigation of myeloid differentiation.
Asunto(s)
Transformación Celular Neoplásica/efectos de los fármacos , Dimetilsulfóxido/farmacología , Leucemia Eritroblástica Aguda/patología , Leucocitos/efectos de los fármacos , Línea Celular , Células Cultivadas , Relación Dosis-Respuesta a Droga , Humanos , Factores de TiempoRESUMEN
Natural cold isolymphocytotoxins (NCILCT) were studied in sera of patients with chronic renal insufficiency who had undergone 3-200 hemodialyses, in sera of healthy subjects before and after intramuscular injections of 5-9 ml of whole autologous blood, and in sera of hemophilia patients before and after a transfusion of 130-250 ml of freshly drawn donor plasma concentrate. It was noted NCILCT activity rose from 20-50% to 50-100% dead cells after injections of autologous blood and plasma transfusions in the majority of cases. In subjects who had undergone hemodialysis from 50 to 200 time NCILCT activity was considerably higher (50-80% dead cells) compared to patients with 3-10 hemodialyses (20-30% dead cells). It was suggested that the graft protection effect of blood transfusions and hemodialysis on kidney graft may be associated with an increase in NCILCT activity.
Asunto(s)
Suero Antilinfocítico/inmunología , Frío , Linfocitos B/inmunología , Citotoxicidad Inmunológica , Humanos , Inmunidad Innata , Fallo Renal Crónico/inmunología , Fallo Renal Crónico/terapia , Trasplante de Riñón , Linfocitos T/inmunologíaRESUMEN
Activity of natural cold isolymphocytotoxins (NCILCT) was studied in the serum of 16 volunteers who received whole auto blood injections. Autohemostimulation was shown to result in the increased activity of NCILCT. The evidence obtained allowed the conclusion that autohemostimulation can be used for preparation of active NCILCT test sera.