Changes in insulin-like growth factor I, 17-b-estradiol, and progesterone in postmenopausal women with benign and malignant ovarian tumours.

BACKGROUND: The aim of this research was to observe changes in IGF-I and steroid hormones in postmenopausal women with benign and malignant ovarian tumors. MATERIAL AND METHODS: The concentrations of insulin-like growth factor I (IGF-I), 17-b-estradiol and progesterone were examined by radioimmunological assay in three groups of postmenopausal women: patients with malignant ovarian tumors, patients with benign ovarian tumors, and healthy women. In all three groups the age and body mass index (BMI) were comparable. RESULTS: The concentrations of IGF-I in the serum of the patients with malignant cancer were significantly lower. The concentrations of estradiol were significantly higher in the patients with both benign and malignant ovarian tumors as compared to the controls. CONCLUSIONS: Decreased IGF-I concentration in the serum of postmenopausal women with ovarian tumors may suggest that the tumor is malignant.

Function of insulin-like growth factor (IGF-I) and its binding protein (IGFBP-1) in pathological proliferation of endometrium.

The purpose of this project was the investigation of insulin, IGF-I and IGFBP-1 concentrations in the serum of patients with pathological changes in endometrium. IGF-I and its binding protein (IGFBP-1) stimulate cell growth and differentiation of the normal and neoplastic endometrium. The mean values of IGF-I concentrations in the group of patients with atypical hyperplasia and endometrial carcinoma were significantly higher than in the control group with normal endometrium. Significantly higher IGFBP-1 concentrations were found in the serum of patients with the atypical hyperplasia of endometrium and endometrial carcinoma. The correlation between insulin concentration and changes in endometrium in the examined groups was not found.

[The influence of demographic and behavioral factors on the risk of human papillomavirus infection]. / Ocena wplywu wybranych czynników demograficznych i srodowiskowych na ryzyko wystapienia infekcji wirusami brodawczaka ludzkiego.

OBJECTIVES: The aim of this work was the assessment of correlation between different types of HPV and chosen factors: the number of pregnancies, childbirths and smoking habits. MATERIALS AND METHODS: Patients were examined in the Gynaecology Clinic, Silesian Medical Academy in Zabrze from 1998 until 2000. They were either hospital patients or women subjected to routine cytological examination. The smears were simultaneously collected for both cytooncologic examination and PCR identification of HPV viruses. Cytological smears were classified according to the Bethesda system. RESULTS: A relationship between the presence of HPV and number of pregnancies or childbirths was confirmed as well as the relationship between smoking and the presence of HPV genome. The higher number of pregnancies or childbirths the higher frequency of HPV presence is. HPV presence was more frequent in the group of smoking women. CONCLUSIONS: A relationship between the presence of HPV and the number of past childbirth or pregnancies was found. The statistically significant correlation between smoking and the risk of HPV infection was also confirmed.

[Chromosome instability in women with genital organs carcinoma]. / Niestabilnosc chromosomowa kobiet z choroba nowotworowa narzadu rodnego.

OBJECTIVES: The aim of the current work was the assessment of the mutagen susceptibility of chromosomes of patients with carcinomas in comparison to healthy volunteers. It was interesting whether the bleomycin assay can be useful for searching for more susceptible to cancer disease individuals. MATERIALS AND METHODS: There were 4 groups of patients analysed: controls and three test groups (patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma). In total 108 female patients were examined by use of bleomycin assay. Lymphocytes were cultured in vitro and treated with bleomycin. The b/c (breaks per cell) index was evaluated by use of light microscopy. RESULTS: Statistically significant increased test values were found in patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma. CONCLUSIONS: The assessment of chromosome instability could be a useful prognostic test in the diagnosis of carcinoma of female genital organs. The bleomycin assay is useful for searching subpopulations with higher chromosome instability and more susceptible to cancer disease.

[Diagnostic procedures of pathological bleeding in women population: comparison of hysteroscopy, ultrasonography and microscopic examination endometrium]. / Diagnostyka nieprawidlowych krwawien z dróg rodnych u kobiet: porównanie badan histeroskopowych, ultrasonograficznych i mikroskopowych endometrium.

The goal of this study was to asses the diagnostic value of hysteroscopy, cytology, ultrasonography and histopathology in various pathological states in endometrium. 250 patients with abnormal uterine bleeding were examined. The wide range of several diagnoses were achieved with 8 cases of cancer. Used methods were found to be complementary because 7 cases of cancer were recognised by histopathologic method, 6 by hysteroscopy and 5 by a cytological test (3 results were suspicious). To the risk group were qualified by ultrasonography all patients in postmenopausal age.

[Detection of human papillomavirus genome in cervical squamous intraepithelial lesions]. / Badania nad obecnoscia genomu onkogennych typów wirusa brodawczaka ludzkiego w wybranych stanach patologii szyjki macicy.

OBJECTIVES: The aim of this work was the assessment of the correlation between different types of HPV and abnormal stages of uterine cervix. MATERIALS AND METHODS: The smears were simultaneously collected for both cytooncologic examination and PCR identification of HPV viruses. Cytologic smears were classified according to the Bethesda system. RESULTS: A strong relationship between the presence of HPV types 16, 18 and 33 and the intensification of cytologic changes was confirmed. The more advanced abnormal changes in the uterine cervix, the more often presence of these HPV types. The presence of HPV types 6 and 11 were more often in low grade lesions. CONCLUSIONS: The conclusions from this examinations are: there is a proportional correlation between the grades of Squamous Intraepithelial Lesions, uterine cervix carcinoma and the presence of oncogenic types of human papillomavirus.

[Comparison of conservative and laparoscopic treatment of PCOS]. / Porównanie leczenia zachowawczego i laparoskopowego zespolu PCOS.

OBJECTIVE: Aim of study contained clinical analysis of results conservative (Clostilbegyt) and laparoscopic (electro-catheterisation of ovaries) methods of treatment PCOS. Authors analysed count of pregnancy, deliveries and serum concentrations of LH, FSH, LH/FSH ratio, 17 beta E2 before and after treatment. MATERIAL AND METHODS: Stady included 50 obese patients (group A, BMI > 28) treated by Clostilbegyt and 50 lean patients (group B, BMI < 28) treated by laparoscopic ovarian electrocautery. RESULTS: We found higher percentage of pregnancies (84%) and deliveries in group B (72%) versus A. We obtained statistically significant higher serum concentrations of LH, 17 beta E2 and LH/FSH ratio of group B versus A. CONCLUSIONS: 1. Recommendation method for treatment PCOS is a laparoscopic electro-catheterisation of ovaries. 2. This method stabilize hormonal secretion of hypothalamic-pituitary-ovarian axis in the PCOS.

[Laparotomy performed in pregnant women due to non-obstetrical causes]. / Laparotomie wykonane u ciezarnych z przyczyn niepolozniczych.

OBJECTIVE: The clinical characteristics of laparotomy during pregnancy were investigated. MATERIALS AND METHODS: A series of 24 cases of laparotomy during pregnancy was investigated with respect to clinicopathologic features and outcome. RESULTS: Among 23 cases there were 20 ovarian tumors, 2 acute appendicitis and 2 uterine fibroid. Gestational ages at the time of surgery ranged from 16 to 24 weeks of patients with ovarian tumors and from 30 to 32 weeks in others. No intraoperative or postoperative maternal or fetal complications occurred. One patient had a stage III ovarian carcinoma and went to total hysterectomy, and other patient had a stage IA ovarian carcinoma. CONCLUSIONS: Appendectomy can be performed without additional risk to the fetus for those who require surgical intervention during pregnancy. Ovarian surgery in pregnancy for persistent masses is important to obtain a final histologic diagnosis.

New renin inhibitors with hydrophilic C-terminus.

Four new peptide-based renin inhibitors, Boc-Phe(4-OMe)-MePhe-AHPPA-epsilon Ahx-EA (11), Boc-Phe(4-OMe)-MeLeu-AHP-PA-epsilon Ahx-EA (15), Boc-Phe(4-OMe)-MePhe-Sta-epsilon Ahx-EA (20) and Boc-Phe(4-OMe)-MeLeu-Sta-epsilon Ahx-EA (21) have been synthesized in search of structures with improved biological properties. They were designed as compounds with moderate hydrophobicity (5.28, 4.79, 4.79 and 4.30), respectively. All synthesized inhibitors were resistant to chymotrypsin activity, all were poorly soluble in buffers pH 2.0 and pH 7.4. The inhibitory potency of renin activity in vitro of 11, 15, 20 and 21 expressed as IC50 was 7.0 x 10(-4), 7.5 x 10(-5), 6.0 x 10(-4) and 2.5 x 10(-4) M/l, respectively.

Renin inhibitors containing nicotinic or isonicotinic acid at the N-terminus. Part V.

Four new compounds: Nic-Phe/4-OMe/-MePhe-Sta-epsilonAhx-OMe/23/,Nic-Phe/4-OMe/-MePhe- Sta-epsilonAhx-Iaa/24/,iNic-Phe/4-OMe/-MeLeu-Sta-ep silonAhx-OMe/29/ and iNic-Phe/4-OMe/-MeLeu-Sta-epsilonAhx-Iaa/30/ have been synthesized in search after renin inhibitors of improved biological properties. Their stability against chymotrypsin activity, solubility in water at pH 7.4, 6.9 and 2.0, partition coefficient and activity in vitro were determined. All synthesized inhibitors are resistant to enzymatic degradation, all are very good soluble in water at pH 2.0, poorly soluble at pH 6.9 and insoluble at pH 7.4. Partition coefficients go up together with increase of pH worth of buffer. IC50 of obtained inhibitors 23,24,29 and 30 is 3 x 10(-4),7.5 x 10(-4),4 x 10(5) and 4 x 10(-3)M/1 respectively.

[Comparative studies of 2 systems of early cyto-oncologic diagnosis of cervix erosion]. / Badania porównawcze dwóch systemów wczesnej diagnostyki cytoonkologicznej nadzerki szyjki macicy.

In the years 1979-1980 screening examinations (by the open method) were given to 51232 women, that is 85.3% of the female population of Bytom at risk for cervical cancer. Grade I and II cytological smears were found in 46320 (98.52%) of women. Papanicolau grade III was found in 201 (1.12%), and grade IV-V in 64 (0.12%). At the same time, in the years 1978-1984 in the closed health service institutions 19968 women were examined, and suspected smears (grade III-IV) were noted in 368 cases (1.8%). Histological verification showed cervical dysplasia in 184 (0.36%) and 106 (0.53%) respectively, and cervical carcinoma grade 0-IV 52 (10%) and 156 (0.94%). The author compared the results with similar ones obtained in various regions of Poland and in foreign countries. The author failed to find greater detectability of cervical dysplasia and carcinoma in Silesia in comparison with certain, less industrialized, regions of Poland. The author thinks that the stationary system of cervical cancer prevention has a greater chance of being introduced on condition of reorganization of this system, introduction of training of cytological technicians and organization of centres of cytooncological examinations.

[Preventive gynecological examinations of women employed in the cement industry]. / Profilaktyczne badania ginekologiczne u kobiet zatrudnionych w przemysle cementowym.

Prophylactic gynecologic examinations were carried out in 218 women-workers of a cement plant. The most frequent diseases were: retroflexion of the uterus (55.5%), inflammation of the ovary (50.45%), erosion of the vaginal part of the uterus (41.8%), prolapse of the vaginal walls (39.45%), incontinence (35.32%) and Trichomonas vaginalis (18.7%). The extensive prevalence of the diseases imposes a need to carry on preventive examinations of women employed in cement industry. The authors indicate that certain diseases are obviously due to deficient hygiene.