Fluorescence Hyperspectral Imaging of Oil Samples and Its Quantitative Applications in Component Analysis and Thickness Estimation.

The fast response and analysis of oil spill accidents is important but remains challenging. Here, a compact fluorescence hyperspectral system based on a grating-prism structure able to perform component analysis of oil as well as make a quantitative estimation of oil film thickness is developed. The spectrometer spectral range is 366⁻814 nm with a spectral resolution of 1 nm. The feasibility of the spectrometer system is demonstrated by determining the composition of three types of crude oil and various mixtures of them. The relationship between the oil film thickness and the fluorescent hyperspectral intensity is furthermore investigated and found to be linear, which demonstrates the feasibility of using the fluorescence data to quantitatively measure oil film thickness. Capable of oil identification, distribution analysis, and oil film thickness detection, the fluorescence hyperspectral imaging system presented is promising for use during oil spill accidents by mounting it on, e.g., an unmanned aerial vehicle.

Alpha11 beta1 integrin-dependent regulation of periodontal ligament function in the erupting mouse incisor.

The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated a null allele of the alpha11 gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective incisors. Mutant incisors are characterized by disorganized periodontal ligaments, whereas molar ligaments appear normal. The primary defect in the incisor ligament leads to halted tooth eruption. alpha11beta1-defective embryonic fibroblasts displayed severe defects in vitro, characterized by (i) greatly reduced cell adhesion and spreading on collagen I, (ii) reduced ability to retract collagen lattices, and (iii) reduced cell proliferation. Analysis of matrix metalloproteinase in vitro and in vivo revealed disturbed MMP13 and MMP14 synthesis in alpha11(-/-) cells. We show that alpha11beta1 is the major receptor for collagen I on mouse embryonic fibroblasts and suggest that alpha11beta1 integrin is specifically required on periodontal ligament fibroblasts for cell migration and collagen reorganization to help generate the forces needed for axial tooth movement. Our data show a unique role for alpha11beta1 integrin during tooth eruption.

Strong resonant coupling of surface plasmon polaritons to radiation modes through a thin metal slab with dielectric gratings.

Strong resonant coupling of surface plasmon polaritons to radiation modes by means of a dielectric grating deposited on top of a metal slab is numerically analyzed, and some novel properties of this configuration are discussed. The dielectric grating is not only responsible for coupling of incident light to surface plasmon polaritons but also for outcoupling of the surface plasmon polaritons to radiation modes. A key advantage of the configuration presented is that it is not based on conventional attenuated total reflection using a prism with high refractive index.

Commercial applications of nuclear transfer cloning: three examples.

Potential applications of cloning go well beyond the popularly envisioned replication of valuable animals. This is because targeted genetic modifications can be made in donor cells before nuclear transfer. Applications that are currently being pursued include therapeutic protein production in the milk and blood of transgenic cloned animals, the use of cells, tissues and organs from gene-modified animals for transplantation into humans and genetically modified livestock that produce healthier and safer products in an environmentally friendly manner. Commercial and social acceptance of one or more of these early cloning applications will lead to yet unimagined applications of nuclear transfer technology. The present paper summarises progress on three additional applications of nuclear transfer, namely the development of male livestock that produce single-sex sperm, the transfer of immune responses from animals to their clones to permit the production of unlimited supplies of unique polyclonal antibodies, and the generation of genetically modified animals that accurately mimic human diseases for the purpose of developing new therapies. However, the myriad applications of cloning will require appropriate safeguards to ensure safe, humane and responsible outcomes of the technology.

Targeted disruption of the ribosomal protein S19 gene is lethal prior to implantation.

The ribosomal protein S19 (RPS19) is located in the small (40S) subunit and is one of 79 ribosomal proteins. The gene encoding RPS19 is mutated in approximately 25% of patients with Diamond-Blackfan anemia, which is a rare congenital erythroblastopenia. Affected individuals present with decreased numbers or the absence of erythroid precursors in the bone marrow, and associated malformations of various organs are common. We produced C57BL/6J mice with a targeted disruption of murine Rps19 to study its role in erythropoiesis and development. Mice homozygous for the disrupted Rps19 were not identified as early as the blastocyst stage, indicating a lethal effect. In contrast, mice heterozygous for the disrupted Rps19 allele have normal growth and organ development, including that of the hematopoietic system. Our findings indicate that zygotes which are Rps19(-/-) do not form blastocysts, whereas one normal Rps19 allele in C57BL/6J mice is sufficient to maintain normal ribosomal and possibly extraribosomal functions.