RESUMEN
Oxytocin (OXT) neurons project to various brain regions and its receptor expression is widely distributed. Although it has been reported that OXT administration affects cognitive function, it is unclear how endogenous OXT plays roles in cognitive function. The present study examined the role of endogenous OXT in mice cognitive function. OXT neurons were specifically activated by OXT neuron-specific excitatory Designer Receptors Exclusively Activated by Designer Drug expression system and following administration of clozapine-N-oxide (CNO). Object recognition memory was assessed with the novel object recognition task (NORT). Moreover, we observed the expression of c-Fos via immunohistochemical staining to confirm neuronal activity. In NORT, the novel object exploration time percentage significantly increased in CNO-treated mice. CNO-treated mice showed a significant increase in the number of c-Fos-positive cells in the supramammillary nucleus (SuM). In addition, we found that the OXT-positive fibers from paraventricular hypothalamic nucleus (PVN) were identified in the SuM. Furthermore, mice injected locally with CNO into the SuM to activate OXTergic axons projecting from the PVN to the SuM showed significantly increased percentage time of novel object exploration. Taken together, we proposed that object recognition memory in mice could be modulated by OXT neurons in the PVN projecting to the SuM.
Asunto(s)
Hipotálamo , Oxitocina , Animales , Ratones , Hipotálamo/metabolismo , Oxitocina/metabolismo , Núcleo Hipotalámico Paraventricular/metabolismo , Receptores de Oxitocina/metabolismo , Hipotálamo Posterior/metabolismo , Neuronas/metabolismo , Proteínas Proto-Oncogénicas c-fos/metabolismoRESUMEN
Homer is a postsynaptic scaffold protein, which has long and short isoforms. The long form of Homer consists of an N-terminal target-binding domain and a C-terminal multimerization domain, linking multiple proteins within a complex. The short form of Homer only has the N-terminal domain and likely acts as a dominant negative regulator. Homer2a, one of the long form isoforms of the Homer family, expresses with a transient peak in the early postnatal stage of mouse cerebellar granule cells (CGCs); however, the functions of Homer2a in CGCs are not fully understood yet. In this study, we investigated the physiological roles of Homer2a in CGCs using recombinant adenovirus vectors. Overexpression of the Homer2a N-terminal domain construct, which was made structurally reminiscent with Homer1a, altered NMDAR1 localization, decreased NMDA currents, and promoted the survival of CGCs. These results suggest that the Homer2a N-terminal domain acts as a dominant negative protein to attenuate NMDAR-mediated excitotoxicity. Moreover, we identified a novel short form N-terminal domain-containing Homer2, named Homer2e, which was induced by apoptotic stimulation such as ischemic brain injury. Our study suggests that the long and short forms of Homer2 are involved in apoptosis of CGCs.
Asunto(s)
Apoptosis , Cerebelo/citología , Proteínas de Andamiaje Homer/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Isquemia Encefálica/patología , Proteínas de Andamiaje Homer/química , Proteínas de Andamiaje Homer/genética , Ratones Endogámicos ICR , Modelos Biológicos , N-Metilaspartato/metabolismo , Dominios Proteicos , Isoformas de Proteínas/metabolismoRESUMEN
Calcium-dependent activator protein for secretion 1 (CAPS1) is a key molecule in vesicular exocytosis, probably in the priming step. However, CAPS1's role in synaptic plasticity and brain function is elusive. Herein, we showed that synaptic plasticity and learning behavior were impaired in forebrain and/or hippocampus-specific Caps1 conditional knockout (cKO) mice by means of molecular, physiological, and behavioral analyses. Neonatal Caps1 cKO mice showed a decrease in the number of docked vesicles in the hippocampal CA3 region, with no detectable changes in the distribution of other major exocytosis-related molecules. Additionally, long-term potentiation (LTP) was partially and severely impaired in the CA1 and CA3 regions, respectively. CA1 LTP was reinforced by repeated high-frequency stimuli, whereas CA3 LTP was completely abolished. Accordingly, hippocampus-associated learning was severely impaired in adeno-associated virus (AAV) infection-mediated postnatal Caps1 cKO mice. Collectively, our findings suggest that CAPS1 is a key protein involved in the cellular mechanisms underlying hippocampal synaptic release and plasticity, which is crucial for hippocampus-associated learning.
Asunto(s)
Proteínas de Unión al Calcio/fisiología , Hipocampo/fisiología , Aprendizaje/fisiología , Proteínas del Tejido Nervioso/fisiología , Plasticidad Neuronal/fisiología , Animales , Western Blotting , Proteínas de Unión al Calcio/metabolismo , Condicionamiento Clásico , Aprendizaje Discriminativo , Femenino , Hipocampo/metabolismo , Hipocampo/ultraestructura , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Microscopía Electrónica , Proteínas del Tejido Nervioso/metabolismo , Fracciones Subcelulares/metabolismoRESUMEN
The HapMap Project is a major international research effort to construct a resource to facilitate the discovery of relationships between human genetic variations and health and disease. The Ser19Stop single nucleotide polymorphism (SNP) of human phytanoyl-CoA hydroxylase-interacting protein-like (PHYHIPL) gene was detected in HapMap project and registered in the dbSNP. PHYHIPL gene expression is altered in global ischemia and glioblastoma multiforme. However, the function of PHYHIPL is unknown. We generated PHYHIPL Ser19Stop knock-in mice and found that PHYHIPL impacts the morphology of cerebellar Purkinje cells (PCs), the innervation of climbing fibers to PCs, the inhibitory inputs to PCs from molecular layer interneurons, and motor learning ability. Thus, the Ser19Stop SNP of the PHYHIPL gene may be associated with cerebellum-related diseases.
Asunto(s)
Cerebelo/citología , Péptidos y Proteínas de Señalización Intracelular/genética , Polimorfismo de Nucleótido Simple , Células de Purkinje/ultraestructura , Secuencia de Aminoácidos , Animales , Sistemas CRISPR-Cas , Forma de la Célula , Codón de Terminación , Femenino , Técnicas de Sustitución del Gen , Proyecto Mapa de Haplotipos , Humanos , Interneuronas/fisiología , Péptidos y Proteínas de Señalización Intracelular/fisiología , Aprendizaje , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Actividad Motora , Fibras Nerviosas/fisiología , Células de Purkinje/metabolismo , Prueba de Desempeño de Rotación con Aceleración Constante , Alineación de Secuencia , Homología de Secuencia de AminoácidoRESUMEN
Animals can remember a situation associated with an aversive event. Contextual fear memory is initially encoded and consolidated in the hippocampus and gradually consolidated in multiple brain regions over time, including the medial prefrontal cortex (PFC). However, it is not fully understood how PFC neurons contribute to contextual fear memory formation during learning. In the present study, neuronal activity was increased in PFC neurons utilizing the pharmacogenetic hM3Dq-system in male mice. We show that fear expression and memory formation are enhanced by increasing neuronal activity in PFC during conditioning phase. Previous studies showed that the activation of hM3Dq receptor in a subset of amygdala neurons enhanced fear memory formation and biased which neurons are allocated to a memory trace, in which immediate early gene c-fos was preferentially expressed following memory retrieval in these pre-activated neurons. In this study, hM3Dq activation in PFC could not change the probability of c-fos expression in pre-activated neurons flowing memory retrieval. Instead, the number c-fos positive neurons following memory retrieval was significantly increased in the basolateral amygdala. Our results suggest that neuronal activity in PFC at the time of learning modulates fear memory formation and downstream cellular activity at an early phase.
Asunto(s)
Miedo , Memoria , Neuronas/metabolismo , Corteza Prefrontal/metabolismo , Animales , Complejo Nuclear Basolateral/metabolismo , Condicionamiento Clásico , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Proteínas Proto-Oncogénicas c-fos/metabolismo , Receptor Muscarínico M3/genética , Receptor Muscarínico M3/metabolismoRESUMEN
ADP-ribosylation factors (ARFs) are a family of small monomeric GTPases comprising six members categorized into three classes: class I (ARF1, 2, and 3), class II (ARF4 and 5), and class III (ARF6). In contrast to class I and III ARFs, which are the key regulators in vesicular membrane trafficking, the cellular function of class II ARFs remains unclear. In the present study, we generated class II ARF-deficient mice and found that ARF4+/-/ARF5-/- mice exhibited essential tremor (ET)-like behaviors. In vivo electrophysiological recordings revealed that ARF4+/-/ARF5-/- mice of both sexes exhibited abnormal brain activity when moving, raising the possibility of abnormal cerebellar excitability. Slice patch-clamp experiments demonstrated the reduced excitability of the cerebellar Purkinje cells (PCs) in ARF4+/-/ARF5-/- mice. Immunohistochemical and electrophysiological analyses revealed a severe and selective decrease of pore-forming voltage-dependent Na+ channel subunit Nav1.6, important for maintaining repetitive action potential firing, in the axon initial segment (AIS) of PCs. Importantly, this decrease in Nav1.6 protein localized in the AIS and the consequent tremors in ARF4+/-/ARF5-/- mice could be alleviated by the PC-specific expression of ARF5 using adeno-associated virus vectors. Together, our data demonstrate that the decreased expression of the class II ARF proteins in ARF4+/-/ARF5-/- mice, leading to a haploinsufficiency of ARF4 in the absence of ARF5, impairs the localization of Nav1.6 to the AIS and hence reduces the membrane excitability in PCs, resulting in the ET-like movement disorder. We suggest that class II ARFs function in localizing specific proteins, such as Nav1.6, to the AIS.SIGNIFICANCE STATEMENT We found that decreasing the expression of class II ARF proteins, through the generation of ARF4+/-/ARF5-/- mice, impairs Nav1.6 distribution to the axon initial segment (AIS) of cerebellar Purkinje cells (PCs), thereby resulting in the impairment of action potential firing of PCs. The ARF4+/-/ARF5-/- mutant mice exhibited movement-associated essential tremor (ET)-like behavior with pharmacological profiles similar to those in ET patients. The exogenous expression of ARF5 reduced the tremor phenotype and restored the localization of Nav1.6 immunoreactivity to the AIS in ARF4+/-/ARF5-/- mice. Thus, our results suggest that class II ARFs are involved in the localization of Nav1.6 to the AISs in cerebellar PCs and that the reduction of class II ARF activity leads to ET-like movement disorder.
Asunto(s)
Factores de Ribosilacion-ADP/fisiología , Axones/metabolismo , Trastornos del Movimiento/etiología , Canal de Sodio Activado por Voltaje NAV1.6/fisiología , Células de Purkinje/metabolismo , Temblor/etiología , Factores de Ribosilacion-ADP/deficiencia , Factores de Ribosilacion-ADP/genética , Potenciales de Acción , Animales , Dependovirus/genética , Electroencefalografía , Electromiografía , Vectores Genéticos/genética , Vectores Genéticos/uso terapéutico , Genotipo , Movimientos de la Cabeza , Ratones , Ratones Endogámicos C57BL , Ratones Noqueados , Trastornos del Movimiento/metabolismo , Trastornos del Movimiento/fisiopatología , Canal de Sodio Activado por Voltaje NAV1.6/deficiencia , Técnicas de Placa-Clamp , Transporte de Proteínas , Células de Purkinje/fisiología , Prueba de Desempeño de Rotación con Aceleración Constante , Método Simple Ciego , Temblor/metabolismo , Temblor/fisiopatologíaRESUMEN
The Ca(2+)-dependent activator protein for secretion 1 (CAPS1) protein plays a regulatory role in the dense-core vesicle exocytosis pathway. To clarify the functions of this protein in the brain, we searched for novel interaction partners of CAPS1 by mass spectrometry. We identified a specific interaction of CAPS1 with septin family proteins. We also demonstrated that the C-terminal region of the CAPS1 protein binds to part of the deduced GTP-binding domain of septin proteins. It is possible that a tertiary complex of septin, CAPS, and syntaxin contributes to dense-core vesicle trafficking and exocytosis in neurons.
Asunto(s)
Encéfalo/metabolismo , Proteínas de Unión al Calcio/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Septinas/metabolismo , Animales , Células COS , Línea Celular Tumoral , Chlorocebus aethiops , Ratones , Unión Proteica , Dominios y Motivos de Interacción de ProteínasRESUMEN
Amphotericin B (AmB) is a polyene antibiotic and reported to be one of a few reagents having therapeutic effects on prion diseases, such as the delay in the appearing of the clinical signs and the prolongation of the survival time. In prion diseases, glial cells have been suggested to play important roles by proliferating and producing various factors such as nitric oxide, proinflammatory cytokines, and neurotrophic factors. However, the therapeutic mechanism of AmB on prion diseases remains elusive. We have previously reported that AmB changed the expression of neurotoxic and neurotrophic factors in microglia (Motoyoshi et al., 2008, Neurochem. Int. 52, 1290-1296). In the present study, we examined the effects of AmB on cellular functions of rat cultured astrocytes. We found that AmB could activate astrocytes to produce nitric oxide via inducible nitric oxide synthase induction. AmB also induced mRNA expression of interleukin-1ß and tumor necrosis factor-α, and productions of their proteins in astrocytes. Moreover, AmB changed levels of neurotrophic factor mRNAs and proteins. Among three neurotrophic factors examined here, neurotrophin-3 mRNA expression and its protein production in the cells were down-regulated by AmB stimulation. On the other hand, AmB significantly enhanced the amounts of glial cell line-derived neurotrophic factor and brain-derived neurotrophic factor proteins in the cells and the medium. These results suggest that AmB might show therapeutic effects on prion diseases by controlling the expression and production of such mediators in astrocytes.
Asunto(s)
Anfotericina B/farmacología , Astrocitos/efectos de los fármacos , Citocinas/biosíntesis , Factores de Crecimiento Nervioso/biosíntesis , Óxido Nítrico/biosíntesis , Animales , Astrocitos/citología , Astrocitos/metabolismo , Secuencia de Bases , Células Cultivadas , Cartilla de ADN , Ratas , Ratas WistarRESUMEN
Phospholipase D4 (PLD4) is a recently identified protein that is mainly expressed in the ionized calcium binding adapter molecule 1 (Iba1)-positive microglia in the early postnatal mouse cerebellar white matter. Unlike PLD1 and PLD2, PLD4 exhibits no enzymatic activity for conversion of phosphatidylcholine into choline and phosphatidic acid, and its function is completely unknown. In the present study, we examined the distribution of PLD4 in mouse cerebellar white matter during development and under pathological conditions. Immunohistochemical analysis revealed that PLD4 expression was associated with microglial activation under such two different circumstances. A primary cultured microglia and microglial cell line (MG6) showed that PLD4 was mainly present in the nucleus, except the nucleolus, and expression of PLD4 was upregulated by lipopolysaccharide (LPS) stimulation. In the analysis of phagocytosis of LPS-stimulated microglia, PLD4 was co-localized with phagosomes that contained BioParticles. Inhibition of PLD4 expression using PLD4 specific small interfering RNA (siRNA) in MG6 cells significantly reduced the ratio of phagocytotic cell numbers. These results suggest that the increased PLD4 in the activation process is involved in phagocytosis of activated microglia in the developmental stages and pathological conditions of white matter.
Asunto(s)
Encéfalo/metabolismo , Glicoproteínas de Membrana/metabolismo , Microglía/metabolismo , Fagocitosis , Animales , Western Blotting , Encéfalo/citología , Proliferación Celular , Células Cultivadas , Ensayo de Inmunoadsorción Enzimática , Exonucleasas , Técnicas para Inmunoenzimas , Hibridación in Situ , Lipopolisacáridos/farmacología , Glicoproteínas de Membrana/antagonistas & inhibidores , Glicoproteínas de Membrana/genética , Ratones , Ratones Endogámicos C57BL , Microglía/citología , ARN Mensajero/genética , ARN Interferente Pequeño/genética , Reacción en Cadena en Tiempo Real de la PolimerasaRESUMEN
In adult rat brains, brain-derived neurotrophic factor (BDNF) rhythmically oscillates according to the light-dark cycle and exhibits unique functions in particular brain regions. However, little is known of this subject in juvenile rats. Here, we examined diurnal variation in BDNF and neurotrophin-3 (NT-3) levels in 14-day-old rats. BDNF levels were high in the dark phase and low in the light phase in a majority of brain regions. In contrast, NT-3 levels demonstrated an inverse phase relationship that was limited to the cerebral neocortex, including the visual cortex, and was most prominent on postnatal day 14. An 8-h phase advance of the light-dark cycle and sleep deprivation induced an increase in BDNF levels and a decrease in NT-3 levels in the neocortex, and the former treatment reduced synaptophysin expression and the numbers of synaptophysin-positive presynaptic terminals in cortical layer IV and caused abnormal BDNF and NT-3 rhythms 1 week after treatment. A similar reduction of synaptophysin expression was observed in the cortices of Bdnf gene-deficient mice and Ca(2+)-dependent activator protein for secretion 2 gene-deficient mice with abnormal free-running rhythm and autistic-like phenotypes. In the latter mice, no diurnal variation in BDNF levels was observed. These results indicate that regular rhythms of BDNF and NT-3 are essential for correct cortical network formation in juvenile rodents.
Asunto(s)
Factor Neurotrófico Derivado del Encéfalo/metabolismo , Regulación de la Expresión Génica , Neurotrofina 3/metabolismo , Animales , Encéfalo/metabolismo , Calcio/metabolismo , Corteza Cerebral/metabolismo , Masculino , Ratones , Modelos Neurológicos , Fotoperiodo , Proteínas Proto-Oncogénicas c-akt/metabolismo , Ratas , Ratas Sprague-Dawley , Sinapsis/metabolismoRESUMEN
The cerebellar cortical circuit of mammals develops via a series of magnificent cellular events in the postnatal stage of development to accomplish the formation of functional circuit architectures. The contribution of genetic factors is thought to be crucial to cerebellar development. Therefore, it is essential to analyze the underlying transcriptome during development to understand the genetic blueprint of the cerebellar cortical circuit. In this review, we introduce the profiling of large numbers of spatiotemporal gene expression data obtained by developmental time-series microarray analyses and in situ hybridization cellular mRNA mapping, and the creation of a neuroinformatics database called the Cerebellar Development Transcriptome Database. Using this database, we have identified thousands of genes that are classified into various functional categories and are expressed coincidently with related cellular developmental stages. We have also suggested the molecular mechanisms of cerebellar development by functional characterization of several identified genes (Cupidin, p130Cas, very-KIND, CAPS2) responsible for distinct cellular events of developing cerebellar granule cells. Taken together, the gene expression profiling during the cerebellar development demonstrates that the development of cerebellar cortical circuit is attributed to the complex but orchestrated transcriptome.
Asunto(s)
Cerebelo/crecimiento & desarrollo , Regulación del Desarrollo de la Expresión Génica , Animales , Proteínas Reguladoras de la Apoptosis/fisiología , Proteínas de Unión al Calcio/genética , Proteínas de Unión al Calcio/fisiología , Proteínas Portadoras/genética , Clonación Molecular , Proteína Sustrato Asociada a CrK/genética , Proteína Sustrato Asociada a CrK/fisiología , Bases de Datos Genéticas , Exonucleasas , Perfilación de la Expresión Génica , Factores de Intercambio de Guanina Nucleótido/genética , Factores de Intercambio de Guanina Nucleótido/fisiología , Proteínas de Andamiaje Homer , Glicoproteínas de Membrana/fisiología , Ratones , Proteínas de la Mielina/fisiología , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/fisiología , Neuronas/fisiología , Análisis de Secuencia por Matrices de Oligonucleótidos , Proteínas Tirosina Quinasas/fisiología , Células de Purkinje/fisiología , Sinapsis/genética , Factores de Transcripción/genéticaRESUMEN
Ca(2+)-dependent activator protein for secretion (CAPS) regulates exocytosis of catecholamine- or neuropeptide-containing dense-core vesicles (DCVs) at secretion sites, such as nerve terminals. However, large amounts of CAPS protein are localized in the cell soma, and the role of somal CAPS protein remains unclear. The present study shows that somal CAPS1 plays an important role in DCV trafficking in the trans-Golgi network. The anti-CAPS1 antibody appeared to pull down membrane fractions, including many Golgi-associated proteins, such as ADP-ribosylation factor (ARF) small GTPases. Biochemical analyses of the protein-protein interaction showed that CAPS1 interacted specifically with the class II ARF4/ARF5, but not with other classes of ARFs, via the pleckstrin homology domain in a GDP-bound ARF form-specific manner. The pleckstrin homology domain of CAPS1 showed high affinity for the Golgi membrane, thereby recruiting ARF4/ARF5 to the Golgi complex. Knockdown of either CAPS1 or ARF4/ARF5 expression caused accumulation of chromogranin, a DCV marker protein, in the Golgi, thereby reducing its DCV secretion. In addition, the overexpression of CAPS1 binding-deficient ARF5 mutants induced aberrant chromogranin accumulation in the Golgi and consequently reduced its DCV secretion. These findings implicate a functional role for CAPS1 protein in the soma, a major subcellular localization site of CAPS1 in many cell types, in regulating DCV trafficking in the trans-Golgi network; this activity occurs via protein-protein interaction with ARF4/ARF5 in a GDP-dependent manner.
Asunto(s)
Factores de Ribosilacion-ADP/metabolismo , Proteínas de Unión al Calcio/metabolismo , Aparato de Golgi/metabolismo , Vesículas Secretoras/metabolismo , Proteínas de Transporte Vesicular/metabolismo , Factores de Ribosilacion-ADP/genética , Animales , Transporte Biológico/fisiología , Proteínas de Unión al Calcio/genética , Técnicas de Silenciamiento del Gen , Aparato de Golgi/genética , Guanosina Difosfato/genética , Guanosina Difosfato/metabolismo , Ratones , Células PC12 , Estructura Terciaria de Proteína , Ratas , Vesículas Secretoras/genética , Proteínas de Transporte Vesicular/genéticaRESUMEN
Apoptosis-associated tyrosine kinase 1 (AATYK1), also named LMTK1, was previously isolated as an apoptosis-related gene from 32Dcl3 myeloid precursor cells, but its precise function remains unknown. AATYK1A, an isoform without a transmembrane domain, is highly expressed in neurons. We identified palmitoylation of AATYK1A at three N-terminal cysteine residues in cortical cultured neurons and COS-7 cells and found that palmitoylation determined localization of AATYK1A to the transferrin receptor-positive recycling endosomes. Further, we identified the tyrosine kinase Src as a novel AATYK1A-interacting protein. Src and Fyn phosphorylated AATYK1A at tyrosines 25 and 46 in a palmitoylation-dependent manner. The association of AATYK1A with Src in endosomes was also found to be palmitoylation-dependent. These results indicate that palmitoylation is a critical factor not only for the subcellular localization of AATYK1A but also for its interaction with Src.
Asunto(s)
Proteínas Reguladoras de la Apoptosis/clasificación , Proteínas Reguladoras de la Apoptosis/fisiología , Apoptosis/fisiología , Endosomas/metabolismo , Lipoilación , Familia-src Quinasas/metabolismo , Animales , Células COS , Células Cultivadas , Corteza Cerebral/citología , Chlorocebus aethiops , Ratones , Neuronas/metabolismo , Familia-src Quinasas/genéticaRESUMEN
Opalin is a transmembrane protein detected specifically in mammalian oligodendrocytes. Opalin homologs are found only in mammals and not in the genome sequences of other animal classes. We first determined the nucleotide sequences of Opalin orthologs and their flanking regions derived from four prosimians, a group of primitive primates. A global comparison revealed that an evolutionarily conserved region exists in the first intron of Opalin. When the conserved domain was assayed for its enhancer activity in transgenic mice, oligodendrocyte-directed expression was observed. In an oligodendroglial cell line, Oli-neu, the conserved domain showed oligodendrocyte-directed expression. The conserved domain is composed of eight subdomains, some of which contain binding sites for Myt1 and cAMP-response element binding protein (CREB). Deletion analysis and cotransfection experiments revealed that the subdomains have critical roles in Opalin gene expression. Over-expression of Myt1, treatment of the cell with leukemia inhibitory factor (LIF), and cAMP analog (CREB activator) enhanced the expression of endogenous Opalin in Oli-neu cells and activated the oligodendrocyte enhancer. These results suggest that LIF, cAMP signaling cascades and Myt1 play significant roles in the differentiation of oligodendrocytes through their action on the Opalin oligodendrocyte enhancer.
Asunto(s)
Diferenciación Celular/fisiología , Intrones/fisiología , Proteínas de la Mielina/genética , Proteínas de la Mielina/metabolismo , Oligodendroglía/fisiología , Filogenia , Regiones Promotoras Genéticas/fisiología , Animales , Diferenciación Celular/efectos de los fármacos , Células Cultivadas , Clonación Molecular , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/metabolismo , Proteína de Unión a Elemento de Respuesta al AMP Cíclico/farmacología , Proteínas de Unión al ADN/genética , Proteínas de Unión al ADN/metabolismo , Genómica , Humanos , Factor Inhibidor de Leucemia/farmacología , Luciferasas/metabolismo , Ratones , Ratones Transgénicos , Oligodendroglía/efectos de los fármacos , Regiones Promotoras Genéticas/efectos de los fármacos , Estructura Terciaria de Proteína , ARN Mensajero/metabolismo , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Strepsirhini , Factores de Transcripción/genética , Factores de Transcripción/metabolismo , TransfecciónRESUMEN
MYO18B is a class XVIII myosin, cloned as a tumor suppressor gene candidate. To investigate the mechanisms of MYO18B-dependent tumor suppression, MYO18B-interacting proteins were searched for by a yeast two-hybrid screen. HOMER2, a Homer/Ves1 family protein, was identified as a binding partner of MYO18B. These proteins co-localized in the regions of membrane protrusion and stress fiber, which are known as ones with filamentous actin-rich structures. Expression of HOMER2 enhanced the ability of MYO18B to suppress anchorage-independent growth. These results indicate that HOMER2 and MYO18B cooperate together in tumor suppression.
Asunto(s)
Proteínas Portadoras/metabolismo , Adhesión Celular/fisiología , Membrana Celular/fisiología , Proliferación Celular , Miosinas/metabolismo , Proteínas Supresoras de Tumor/metabolismo , Animales , Proteínas de Andamiaje Homer , Ratones , Células 3T3 NIH , Unión ProteicaRESUMEN
Crk-associated substrate (Cas) is a tyrosine-phosphorylated docking protein that is indispensable for the regulation of the actin cytoskeletal organization and cell migration in fibroblasts. The function of Cas in neurons, however, is poorly understood. Here we report that Cas is dominantly enriched in the brain, especially the cerebellum, of postnatal mice. During cerebellar development, Cas is highly tyrosine phosphorylated and is concentrated in the neurites and growth cones of granule cells. Cas coimmunoprecipitates with Src family protein tyrosine kinases, Crk, and cell adhesion molecules and colocalizes with these proteins in granule cells. The axon extension of granule cells is inhibited by either RNA interference knockdown of Cas or overexpression of the Cas mutant lacking the YDxP motifs, which are tyrosine phosphorylated and thereby interact with Crk. These findings demonstrate that Cas acts as a key scaffold that links the proteins associated with tyrosine phosphorylation signaling pathways to the granule cell axon elongation.
Asunto(s)
Axones/fisiología , Cerebelo/crecimiento & desarrollo , Proteína Sustrato Asociada a CrK/metabolismo , Conos de Crecimiento/metabolismo , Familia-src Quinasas/metabolismo , Secuencia de Aminoácidos , Animales , Cadherinas/metabolismo , Cerebelo/citología , Cerebelo/metabolismo , Proteína Sustrato Asociada a CrK/análisis , Proteína Sustrato Asociada a CrK/genética , Gránulos Citoplasmáticos/metabolismo , Ratones , Datos de Secuencia Molecular , Mutación , Fosforilación , Estructura Terciaria de Proteína , Proteínas Proto-Oncogénicas c-crk/metabolismo , ARN Mensajero/análisis , ARN Mensajero/metabolismo , Transducción de Señal , Tirosina/metabolismoRESUMEN
Human bone marrow-derived mesenchymal stem cells (hMSCs) have the potential to differentiate into several types of cells. Calcium ions (Ca(2+)) play an important role in the differentiation and proliferation of hMSCs. We have demonstrated that spontaneous [Ca(2+)](i) oscillations occur without agonist stimulation in hMSCs. However, the precise mechanism of its generation remains unclear. In this study, we investigated the mechanism and role of spontaneous [Ca(2+)](i) oscillations in hMSCs and found that IP(3)-induced Ca(2+) release is essential for spontaneous [Ca(2+)](i) oscillations. We also found that an ATP autocrine/paracrine signaling pathway is involved in the oscillations. In this pathway, an ATP is secreted via a hemi-gap-junction channel; it stimulates the P(2)Y(1) receptors, resulting in the activation of PLC-beta to produce IP(3). We were able to pharmacologically block this pathway, and thereby to completely halt the [Ca(2+)](i) oscillations. Furthermore, we found that [Ca(2+)](i) oscillations were associated with NFAT translocation into the nucleus in undifferentiated hMSCs. Once the ATP autocrine/paracrine signaling pathway was blocked, it was not possible to detect the nuclear translocation of NFAT, indicating that the activation of NFAT is closely linked to [Ca(2+)](i) oscillations. As the hMSCs differentiated to adipocytes, the [Ca(2+)](i) oscillations disappeared and the translocation of NFAT ceased. These results provide new insight into the molecular and physiological mechanism of [Ca(2+)](i) oscillations in undifferentiated hMSCs.
Asunto(s)
Adenosina Trifosfato/fisiología , Comunicación Autocrina/fisiología , Señalización del Calcio/fisiología , Células Madre Mesenquimatosas/metabolismo , Factores de Transcripción NFATC/metabolismo , Comunicación Paracrina/fisiología , Adenosina Trifosfato/metabolismo , Adipogénesis/fisiología , Animales , Núcleo Celular/metabolismo , Células Cultivadas , Uniones Comunicantes/metabolismo , Humanos , Modelos Biológicos , Receptores Purinérgicos/fisiología , Factores de Transcripción/metabolismo , TransfecciónRESUMEN
In dissociated cultures of cerebellar granule cells, extracellular high potassium (HK) and low potassium (LK) concentrations control cell survival and apoptosis, respectively. Apoptosis-associated tyrosine kinase (AATYK) is up-regulated during the LK-induced apoptosis. Overexpression of wild-type AATYK, but not its kinase-deficient mutant, stimulates apoptosis in LK. In this study, we analyzed the relationship between the phosphorylation states of AATYK and the survival of granule cells. AATYK was hypophosphorylated in HK, whereas it was hyperphosphorylated in apoptotic LK. HK-dependent hypophosphorylation of AATYK was controlled by L-type voltage-dependent calcium channel-mediated Ca2+ influx followed by Ca2+-dependent protein phosphatase activity. However, LK-induced hyperphosphorylation of AATYK at multiple sites was blocked by kainate, lithium, and protein kinase C-delta inhibitor. AATYK phosphorylation was concurrent with c-Jun phosphorylation. In addition, mutations of AATYK on either the kinase domain or Ser-480, Ser-558, and Ser-566 residues suppressed the LK-induced hyperphosphorylation and apoptosis, suggesting the involvement of self-kinase activity and these Ser residues in this process. Our data therefore indicate that the phosphorylation states of AATYK are closely related to the HK-induced survival and LK-induced apoptosis of cerebellar granule cells.
Asunto(s)
Proteínas Reguladoras de la Apoptosis/fisiología , Apoptosis , Calcio/metabolismo , Potasio/farmacología , Proteínas Tirosina Quinasas/fisiología , Animales , Western Blotting , Canales de Calcio/metabolismo , Movimiento Celular , Supervivencia Celular , Células Cultivadas , Cerebelo/metabolismo , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos/farmacología , Litio/farmacología , Ratones , Mutación , Fosforilación , Plásmidos/metabolismo , Estructura Terciaria de Proteína , Proteínas Proto-Oncogénicas c-jun/metabolismo , Serina/química , Ácido Valproico/farmacologíaRESUMEN
We isolated cDNAs encoding type 2 and type 3 inositol 1,4,5-trisphosphate (IP(3)) receptors (IP(3)R2 and IP(3)R3, respectively) from mouse lung and found a novel alternative splicing segment, SI(m2), at 176-208 of IP(3)R2. The long form (IP(3)R2 SI(m2)(+)) was dominant, but the short form (IP(3)R2 SI(m2)(-)) was detected in all tissues examined. IP(3)R2 SI(m2)(-) has neither IP(3) binding activity nor Ca(2+) releasing activity. In addition to its reticular distribution, IP(3)R2 SI(m2)(+) is present in the form of clusters in the endoplasmic reticulum of resting COS-7 cells, and after ATP or Ca(2+) ionophore stimulation, most of the IP(3)R2 SI(m2)(+) is in clusters. IP(3)R3 is localized uniformly on the endoplasmic reticulum of resting cells and forms clusters after ATP or Ca(2+) ionophore stimulation. IP(3)R2 SI(m2)(-) does not form clusters in either resting or stimulated cells. IP(3) binding-deficient site-directed mutants of IP(3)R2 SI(m2)(+) and IP(3)R3 fail to form clusters, indicating that IP(3) binding is involved in the cluster formation by these isoforms. Coexpression of IP(3)R2 SI(m2)(-) prevents stimulus-induced IP(3)R clustering, suggesting that IP(3)R2 SI(m2)(-) functions as a negative coordinator of stimulus-induced IP(3)R clustering. Expression of IP(3)R2 SI(m2)(-) in CHO-K1 cells significantly reduced ATP-induced Ca(2+) entry, but not Ca(2+) release, suggesting that the novel splice variant of IP(3)R2 specifically influences the dynamics of the sustained phase of Ca(2+) signals.
Asunto(s)
Empalme Alternativo , Canales de Calcio/genética , Receptores Citoplasmáticos y Nucleares/genética , Adenosina Trifosfato/química , Secuencia de Aminoácidos , Animales , Western Blotting , Células CHO , Células COS , Calcio/metabolismo , Canales de Calcio/química , Línea Celular , Clonación Molecular , Cricetinae , Citoplasma/metabolismo , ADN Complementario/metabolismo , Retículo Endoplásmico/metabolismo , Proteínas Fluorescentes Verdes/metabolismo , Inmunoprecipitación , Receptores de Inositol 1,4,5-Trifosfato , Insectos , Ionóforos/farmacología , Cinética , Pulmón/metabolismo , Ratones , Ratones Endogámicos C57BL , Microscopía Fluorescente , Microsomas/metabolismo , Datos de Secuencia Molecular , Familia de Multigenes , Mutagénesis Sitio-Dirigida , Ratas , Receptores Citoplasmáticos y Nucleares/química , Proteínas Recombinantes/química , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Homología de Secuencia de Aminoácido , Factores de Tiempo , Distribución TisularRESUMEN
By using cDNA subtraction, we identified an extracellular sulfatase (RsulfFP1) from rat oligodendrocyte progenitor cells (OPCs) whose mRNA expression is down-regulated by tumor necrosis factor-alpha. RsulfFP1 mRNA was expressed specifically in the floor plate and the ventral portion of the rat spinal cord at E15. The expression pattern of RsulfFP1 overlapped with the OPCs, which are also located at the ventral region of the ventricular zone. After this stage, RsulfFP1 expression was attenuated, and the OPCs efficiently migrated throughout the spinal cord. The modification of CG-4 cells, a cell line established from rat O2A cells, by RsulfFP1 activated canonical Wnt signaling. Furthermore, the deletion of RsulfFP1 expression by an antisense oligonucleotide caused impairment of OPC migration in rat spinal cord slice culture. Modification of cells by RsulfFP1 resulted in the increased tyrosine phosphorylation of immunoprecipitated beta-catenin, suggesting that sulfation of the extracellular matrix induced by this sulfatase might be responsible for an increase in Wnt signaling that is involved in the migration of OPCs. Thus, the present study revealed that a sulfatase is responsible for the migration of OPCs and activates intracellular mechanisms that regulate migration.