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1.
Healthcare (Basel) ; 11(14)2023 Jul 16.
Artículo en Inglés | MEDLINE | ID: mdl-37510479

RESUMEN

Lifestyle, a major determinant of health status, comprises a number of habits and behaviours that form a part of daily life. People with healthy lifestyles have a better quality of life, suffer less disease, and have a longer life expectancy. This work reports the design and content validation of a questionnaire-the 'PONTE A 100' questionnaire-assessing the lifestyle of adults. This collects information across five dimensions-'Eating Habits', 'Physical Activity', 'Smoking and use of Alcohol and other Drugs', 'Emotional Wellbeing', and 'Safety and Non-intentional Injuries'-via the answering of a total 33 items. Psychometric validation of the instrument's content was obtained via expert opinions. This was performed by two rounds of assessment and involved 34 experts representing different health science disciplines (mean experience, 27.4 ± 9.4 years). At the end of each round, adjustments were made according to their recommendations. Agreement between the experts was examined using the Aiken V test. A final V value of 0.95 (95% CI, 0.90-1.00) was obtained for the questionnaire as a whole, highlighting the validity of its content. The questionnaire would therefore appear to be an appropriate instrument for assessing the lifestyle of adults.

2.
Cancer Causes Control ; 34(8): 635-645, 2023 Aug.
Artículo en Inglés | MEDLINE | ID: mdl-37160832

RESUMEN

PURPOSE: This study aimed to describe the clinical characteristics and wellness programming preferences of cancer survivors from Acres Homes, a historically Black neighborhood in Houston, Texas, with areas of persistent poverty. The goal of this study was to identify opportunities to increase cancer survivor utilization of healthy eating and active living interventions aligned to cancer center community outreach and engagement efforts. METHODS: This multiple methods study included a retrospective review of electronic health record data (n = 413) and qualitative interviews with cancer survivors (n = 31) immediately preceding initiation of healthy eating, active living programming in Acres Homes. RESULTS: This study found Acres Homes survivors have high rates of co-occurrent cardiometabolic disease including obesity (45.0%), diabetes (30.8%), and other related risk factors as well as treatment-related symptoms. Four major concepts emerged from interviews: (1) Factors that influence survivors' ability to eat well and exercise, (2) Current usage of community resources, (3) Interest in relevant programming, and (4) Specific programming preferences. Opportunities for current and future health promotion programming for cancer survivors were explored. CONCLUSION: Strategically tailoring community resources for cancer survivors can provide a more robust network of support to promote healthy eating and active living in this population. This work informed community implementation of evidence-based health interventions in Acres Homes and may support future projects aiming to enhance community-led cancer prevention efforts in historically underserved communities.


Asunto(s)
Supervivientes de Cáncer , Neoplasias , Humanos , Poblaciones Vulnerables , Ejercicio Físico , Sobrevivientes , Estilo de Vida Saludable , Neoplasias/epidemiología
3.
BMJ Open ; 10(9): e037170, 2020 09 10.
Artículo en Inglés | MEDLINE | ID: mdl-32912985

RESUMEN

INTRODUCTION: Elder mistreatment (EM) is a high prevalence threat to the health and well-being of older adults in the USA. Medics are well-positioned to help with identification of older adults at risk for EM, however, field robust screening tools appropriate for efficient, observation-based screening are lacking. Prior work by this team focused on the development and initial pilot testing of an observation-based EM screening tool named detection of elder abuse through emergency care technicians (DETECT), designed to be implemented by medics during the course of an emergency response (911) call. The objective of the present work is to validate and further refine this tool in preparation for clinical dissemination. METHODS AND ANALYSIS: Approximately 59 400 community-dwelling older adults who place 911 calls during the 36-month study observation period will be screened by medics responding to the call using the DETECT tool. Next, a random subsample of 2520 of the 59 400 older adults screened will be selected to participate in a follow-up interview approximately 2 weeks following the completion of the screening. Follow-up interviews will consist of a medic-led semistructured interview designed to assess the older adult's likelihood of abuse exposure, physical/mental health status, cognitive functioning, and to systematically evaluate the quality and condition of their physical and social living environment. The data from 25% (n=648) of these follow-up interviews will be presented to a longitudinal, experts and all data panel for a final determination of EM exposure status, representing the closest proxy to a 'gold standard' measure available. ETHICS AND DISSEMINATION: This study has been reviewed and approved by the Committee for the Protection of Human Subjects at the University of Texas School of Public Health. The results will be disseminated through formal presentations at local, national and international conferences and through publication in peer-reviewed scientific journals.


Asunto(s)
Abuso de Ancianos , Anciano , Atención a la Salud , Abuso de Ancianos/diagnóstico , Humanos , Vida Independiente , Tamizaje Masivo , Prevalencia
4.
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1390201

RESUMEN

RESUMEN Los antidiabéticos orales (ADO) en pacientes con diabetes mellitus 2 y con comorbilidades reumatológicas no han demostrado interacción relevante con fármacos modificadores de la enfermedad (DMARDs), se debe recordar que la toma de varios medicamentos eleva el riesgo de interacciones o efectos adversos sobre todo si estos tienen un metabolismo en el mismo órgano. El objetivo es dar a conocer que interacciones existen entre los ADO y los DMARDs, mediante la búsqueda de artículos de revisión, guías farmacológicas y estudios. El resultado más sobresaliente fue el hallazgo de un estudio que abarca el uso de un DPP-4 (inhibidor de la dipeptidil peptidasa-4) especificamente sitagliptina como efecto protector frente a la toxicidad producida por el metotrexato. En pacientes con enfermedades reumatológicas y que padecen de diabetes mellitus 2, el uso de la hidroxicloroquina se ha comprobado que logra disminuir los niveles de lípidos, pero no específicamente como interacción medicamentosa con algún ADO, es una propiedad innata de la hidroxicloroquina. Se encontró evidencia de que existe correlación de hipoglicemia en pacientes que toman fármacos secretagogos y sulfazalacina.


ABSTRACT Oral antidiabetics drugs (OADs) in patients with diabetes mellitus 2 and with rheumatic comorbidities have not demonstrated relevant interaction with disease-modifying drugs (DMARDs). It should be remembered that taking several medications increases the risk of interactions or adverse effects especially if these have their metabolism in the same organ. The objective is to make known what interactions exist between OADs and DMARDs, by searching for review articles, pharmacological guides and studies. The most outstanding result was the finding of a study that covers the use of a DPP-4 (dipeptidyl peptidase-4 inhibitor), specifically sitagliptin as a protective effect against the toxicity produced by methotrexate. In patients with rheumatic diseases who also suffer from diabetes mellitus 2, the use of hydroxychloroquine has been shown to reduce lipid levels, but not specifically as a drug interaction with any OADs, it is an innate property of hydroxychloroquine. Evidence was found that there is a correlation of hypoglycemia in patients taking secretagogue drugs and sulfasalazine.

5.
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1390210

RESUMEN

RESUMEN Paciente femenino de 53 años de edad, que ingreso por cuadro clínico de 10 días de evolución caracterizado por mialgias y edema en extremidades inferiores. Al examen físico: facie abotagada, piel seca, cabello fino, disminución del vello axilar y púbico. En laboratorio se evidencia elevación de enzimas musculares (mioglobina 3000 U/L, CPK 2876 U/L), alteración del Na sérico 112 mEq/L, perfil tiroideo alterado (TSH normal a baja 1,14 UI/mL y FT4 baja 0,08 UI/ml), cortisol AM de 7,2 mcg/dL. Se solicito resonancia magnética con protocolo de silla turca se observa; hipófisis disminuida de tamaño en todos sus diámetros que confirma el hallazgo de un síndrome de silla turca parcialmente vacía. La hiponatremia asociada a hipopituitarismo es poco común.


ABSTRACT A 53-year-old female patient was admitted by a clinical case of 10 days of evolution characterized by myalgia and edema in the lower extremities. On physical examination: facial swelling, dry skin, fine hair, decreased axillary and pubic hair. In the laboratory there is evidence of elevation of muscle enzymes (myoglobin 3000 U/L, CPK 2876 U/L), alteration of serum Na 112 mEq/L, altered thyroid profile (normal to low TSH 1.14 IU/mL and FT4 low 0.08 IU/ml), AM cortisol 7.2 mcg/dL. Magnetic resonance imaging is requested with sella Turcica protocol. It is observed a decreased pituitary gland in all its diameters confirming the finding of a partially empty sella syndrome. Hyponatremia associated with hypopituitarism is uncommon.

6.
Rev. ecuat. neurol ; 28(3): 87-93, sep.-dic. 2019. tab, graf
Artículo en Español | LILACS-Express | LILACS | ID: biblio-1058479

RESUMEN

Resumen El síndrome de encefalopatía posterior reversible, es un síndrome clínico-radiológico, caracterizado por imágenes compatibles con edema vasogénico, cuya fisiopatología no está esclarecida por completo. Existen múltiples causas descritas de este síndrome, principalmente hipertensión arterial severa, insuficiencia renal, sepsis, preeclampsia o eclampsia, terapia inmunosupresora, entre otras. Presentamos a continuación el caso de un paciente de 38 años, postrasplante renal en tratamiento con micofenolato y prednisona, con falla renal del trasplante y anemia crónica, hospitalizado por sepsis de foco urinario más tuberculosis ganglionar, quien tras recibir un concentrado de glóbulos rojos presenta convulsiones tónico clónicas, elevación de Hb de 3.1 g/dl e imágenes compatibles con edema vasogénico y resolución casi completa de las mismas a los 16 días.


Abstract Posterior reversible encephalopathy syndrome is a clinical-radiological syndrome, characterized by images compatible with vasogenic edema, whose pathophysiology is not fully clarified. There are multiple described causes of this syndrome, mainly severe arterial hypertension, renal insufficiency, sepsis, preeclampsia or eclampsia, immunosuppressive therapy and others. We report a case of a 38-year-old man, renal post-transplant in treatment with mycophenolate and prednisone, with renal failure of the transplant and chronic anemia, hospitalized by urinary focus sepsis plus lymph node tuberculosis, who after receiving 1 concentrate of red blood cells presents seizures, Hb elevation of 3.1 g/dl and images compatible with vasogenic edema and almost complete resolution of them at 16 days.

7.
Artículo en Inglés | MEDLINE | ID: mdl-31082684

RESUMEN

Sulfur and nitrogen mustards are internationally banned vesicants listed as Schedule 1 chemical agents in the Chemical Weapons Convention. These compounds are highly reactive electrophiles that form stable adducts to a variety of available amino acid residues on proteins upon exposure. We present a quantitative exposure assay that simultaneously measures agent specific protein adducts to cysteine for sulfur mustard (HD) and three nitrogen mustards (HN1, HN2, and HN3). Proteinase K was added to a serum or plasma sample to digest protein adducts and form the target analyte, the blister agent bound to the tripeptide cysteine-proline-phenylalanine (CPF). The mustard adducted-tripeptide was purified by solid phase extraction and analyzed using isotope dilution LC-MS/MS. Product ion structures were identified using high-resolution product ion scan data for HD-CPF, HN1-CPF, HN2-CPF, and HN3-CPF. Thorough matrix comparison, analyte recovery, ruggedness, and stability studies were incorporated during method validation to produce a robust method. The method demonstrated long term-stability, precision (RSD < 15%), and intra- and inter-day accuracies > 85% across the reportable range of 3.00-200 ng/mL for each analyte. Compared to previously published assays, this method quantitates both sulfur and nitrogen mustard exposure biomarkers, requires only 10 µL of sample volume, and can use either a liquid sample or dried sample spot.


Asunto(s)
Exposición a Riesgos Ambientales/análisis , Compuestos de Mostaza/sangre , Albúmina Sérica/química , Biomarcadores/sangre , Biomarcadores/química , Cromatografía Líquida de Alta Presión , Cisteína/sangre , Cisteína/química , Humanos , Compuestos de Mostaza/química , Reproducibilidad de los Resultados , Albúmina Sérica/análisis , Espectrometría de Masas en Tándem
8.
J Elder Abuse Negl ; 31(2): 129-145, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-30614399

RESUMEN

OBJECTIVE: To pilot test the feasibility of implementing an elder abuse (EA) screening tool (DETECT) designed for medics. METHODS: Testing occurred between September 17th, 2015 and October 26th, 2015. MedStar Mobile Healthcare medics completed the DETECT tool when responding to calls for community-dwelling patients 65 years of age or older. RESULTS: The DETECT tool was used 1,248 times by 97% of medics responding to an eligible 911 call. Medics responded affirmatively to at least one screening item on 209 of the completed screenings (16.8%). Immediately following the introduction of the DETECT tool, there was an increase of 5.4 (226% above baseline) reports per month (p = 0.0056). CONCLUSIONS: The DETECT tool was easily incorporated into medic's field-based practice and resulted in an increase in medic generated reports of EA to APS. Future research designed to evaluate the tool's validity and reliability are warranted.


Asunto(s)
Abuso de Ancianos/diagnóstico , Servicios Médicos de Urgencia , Auxiliares de Urgencia , Tamizaje Masivo , Anciano , Femenino , Humanos , Masculino , Proyectos Piloto , Texas
9.
Prev Med ; 114: 168-179, 2018 09.
Artículo en Inglés | MEDLINE | ID: mdl-29981792

RESUMEN

The purpose of this project was to assess the magnitude of the relationship between violence against women and cancer; to identify the exposures and cancers for which this relationship was particularly robust; to identify the effect of violence exposure on cancer screening. We conducted a meta-analysis of 36 studies to determine the relationship between violence against women and cancer outcomes, including screening, in 2017. Results from this review provide evidence of a significant, positive relationship between violence and cancer diagnoses, particularly for cervical cancer. Women who were victims of intimate partner violence and sexual abuse were more likely to be diagnosed with cancer compared with non-victims. Violence against women did not appear to be related to cancer screening practices and routine clinical service utilization; however, violence was associated with greater odds of abnormal pap test results. Victims of intimate partner violence and women who suffered physical abuse were more likely to have abnormal pap test results. In conclusion, use of screening tools for violence against women in clinical settings may improve the breadth and quality of research on violence against women and cancer. Investigators should consider how to creatively apply case-control and retrospective cohort designs to investigate the complex mechanisms and moderators of the relationship between violence against women and cancer.


Asunto(s)
Violencia de Pareja/estadística & datos numéricos , Prueba de Papanicolaou/estadística & datos numéricos , Neoplasias del Cuello Uterino/diagnóstico , Adulto , Femenino , Humanos , Tamizaje Masivo/métodos , Adulto Joven
10.
Prev Med ; 97: 93-99, 2017 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-28111096

RESUMEN

Alcohol and tobacco use during pregnancy are among the strongest and most preventable risk factors for adverse neonatal health outcomes, but few developmentally sensitive, population-based studies of this phenomenon have been conducted. To address this gap, the present study examined the prevalence and correlates of alcohol and tobacco use among pregnant adolescents (aged 12-17) and adults (aged 18-44) in the United States. Data were derived from the population-based National Survey of Drug Use and Health (80,498 adolescent and 152,043 adult women) between 2005 and 2014. Findings show disconcerting levels of past-month use among pregnant women with 11.5% of adolescent and 8.7% of adult women using alcohol, and 23.0% of adolescent and 14.9% of adult women using tobacco. Compared to their non-pregnant counterparts, pregnant adolescents were less likely to report past 30-day alcohol use (AOR=0.52, 95% CI=0.36-0.76), but more likely to report past 30-day tobacco use (AOR=2.20, 95% CI=1.53-3.18). Compared to their non-pregnant adult counterparts, pregnant adults were less likely to report using alcohol (AOR=0.06, 95% CI=0.05-0.07) and tobacco (AOR=0.47, 95% CI=0.43-0.52). Compared to pregnant abstainers, pregnant women reporting alcohol/tobacco use were more likely to have had a major depressive episode in the past 12months, report criminal justice system involvement, and endorse comorbid alcohol/tobacco use. Given alcohol and tobacco's deleterious consequences during pregnancy, increased attention to reducing use is critical. Findings suggest that tobacco use is especially problematic for both adolescents and adults and is strongly linked with depression and criminal justice involvement, especially among adults.


Asunto(s)
Consumo de Bebidas Alcohólicas/epidemiología , Mujeres Embarazadas , Uso de Tabaco/epidemiología , Adolescente , Consumo de Bebidas Alcohólicas/prevención & control , Femenino , Encuestas Epidemiológicas , Humanos , Embarazo , Complicaciones del Embarazo/epidemiología , Complicaciones del Embarazo/psicología , Embarazo en Adolescencia/psicología , Factores de Riesgo , Uso de Tabaco/prevención & control , Estados Unidos/epidemiología
11.
Drug Alcohol Depend ; 169: 5-10, 2016 Dec 01.
Artículo en Inglés | MEDLINE | ID: mdl-27750184

RESUMEN

INTRODUCTION: Prior research has often overlooked potential cohort differences in marijuana views and use across adolescence and young adulthood. To begin to address this gap, we conduct an exploratory examination of marijuana views and use among American youth using a panel of cross-sectional surveys. METHOD: Findings are based on repeated, cross-sectional data collected annually from adolescents (ages 12-17; n=230,452) and young adults (ages 18-21; n=120,588) surveyed as part of the National Survey on Drug Use and Health between 2002 and 2014. For each of the birth years between 1986 and 1996, we combined a series of nationally representative cross-sections to provide multi-year data strings designed to approximate nationally representative cohorts. RESULTS: Compared to youth born in the mid-to-late 1980s, youth born in the mid-1990s reported significantly higher levels of marijuana disapproval during the early adolescent years (Age 14: 1988=64.7%, 1994=70.4%) but lower levels of disapproval during the young adult years (Age 19: 1988=32.0%, 1994=25.0%; Age 20: 1988=27.9%, 1994=19.7%). Moreover, the prevalence of marijuana use among youth born in 1994 was significantly lower-compared to youth born in 1988-at age 14 (1988: 11.39%, 1994: 8.19%) and significantly higher at age 18 (1988: 29.67%, 1994: 34.83%). This pattern held even when adjusting for potential confounding by demographic changes in the population across the study period. CONCLUSIONS: We see evidence of changes in the perceptions of marijuana use among youth born during the late twentieth century.


Asunto(s)
Actitud Frente a la Salud , Cannabis , Fumar Marihuana/psicología , Adolescente , Estudios Transversales , Femenino , Humanos , Masculino , Fumar Marihuana/epidemiología , Prevalencia , Encuestas y Cuestionarios , Estados Unidos , Adulto Joven
12.
BMC Emerg Med ; 16(1): 19, 2016 06 01.
Artículo en Inglés | MEDLINE | ID: mdl-27250247

RESUMEN

BACKGROUND: To develop a screening tool to enhance elder abuse and neglect detection and reporting rates among emergency medical technicians (EMTs). Our primary aim was to identify the most salient indicators of elder abuse and neglect for potential inclusion on a screening tool. We also sought to identify practical elements of the tool that would optimize EMT uptake and use in the field, such as format, length and number of items, and types of response options available. METHODS: Qualitative data were collected from 23 EMTs and Adult Protective Services (APS) caseworkers that participated in one of five semi-structured focus groups. Focus group data were iteratively coded by two coders using inductive thematic identification and data reduction. Findings were subject to interpretation by the research team. RESULTS: EMTs and APS caseworks identified eight domains of items that might be included on a screening tool: (1) exterior home condition; (2) interior living conditions; (3) social support; (4) medical history; (5) caregiving quality; (6) physical condition of the older adult; (7) older adult's behavior; and, (8) EMTs instincts. The screening tool should be based on observable cues in the physical or social environment, be very brief, easily integrated into electronic charting systems, and provide a decision rule for reporting guidance to optimize utility for EMTs in the field. CONCLUSIONS: We described characteristics of a screening tool for EMTs to enhance detection and reporting of elder abuse and neglect to APS. Future research should narrow identified items and evaluate how these domains positively predict confirmed cases of elder abuse and neglect.


Asunto(s)
Abuso de Ancianos/diagnóstico , Auxiliares de Urgencia , Tamizaje Masivo/métodos , Adulto , Anciano , Anciano de 80 o más Años , Cuidadores , Femenino , Grupos Focales , Estado de Salud , Vivienda , Humanos , Masculino , Salud Mental , Persona de Mediana Edad , Investigación Cualitativa , Factores de Riesgo , Apoyo Social , Adulto Joven
13.
Drug Alcohol Depend ; 158: 60-6, 2016 Jan 01.
Artículo en Inglés | MEDLINE | ID: mdl-26613838

RESUMEN

BACKGROUND: Epidemiologic research suggests that 14% of the population do not complete high school, and dropout has been linked to mental health conditions, substance use, chronic health problems, and criminal behavior. Few studies have assessed whether attainment of the general education development (GED) credential is protective from substance use. PURPOSE: To assess the long-term outcomes of school dropout and GED attainment on past year substance use disorders, age of onset, and current smoking status. METHODS: Longitudinal data were included for lifetime substance users who participated in the National Epidemiological Survey on Alcohol and Related Conditions (Waves I and II). Eligible participants (N=30,608) were classified as having completed high school, dropped out of high school and did not complete a GED, or completed GED at Wave I. Survey logistic regression analyses were used to determine whether high school graduation status was associated with substance use disorders and smoking at Wave II. RESULTS: Multivariate results suggest that participants who dropped out of high school (OR=1.53; p<.01) or attained a GED were more likely to have a past year marijuana use disorder (OR=1.62 p<.01) compared to high school graduates. High school dropouts were also more likely to be current smokers (OR=1.88; p<.05) than graduates. CONCLUSIONS: High school dropouts have higher long-term rates of marijuana use disorder and smoking in adulthood than graduates. Attainment of a GED does not appear to be protective from marijuana use disorders in adulthood.


Asunto(s)
Evaluación Educacional , Instituciones Académicas/tendencias , Abandono Escolar/psicología , Trastornos Relacionados con Sustancias/epidemiología , Trastornos Relacionados con Sustancias/psicología , Adolescente , Adulto , Femenino , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Fumar/epidemiología , Fumar/psicología , Fumar/tendencias , Trastornos Relacionados con Sustancias/diagnóstico , Encuestas y Cuestionarios , Factores de Tiempo , Adulto Joven
14.
J Rheumatol ; 42(5): 749-59, 2015 May.
Artículo en Inglés | MEDLINE | ID: mdl-25729037

RESUMEN

OBJECTIVE: To assess surface APRIL (a proliferation-inducing ligand; CD256) expression by circulating myeloid cells in rheumatoid arthritis (RA) and to determine its relationship to disease activity. METHODS: Peripheral blood mononuclear cells (PBMC) and plasma were obtained from patients with RA and healthy donors. PBMC were stained for flow cytometry to detect surface APRIL and blood cell markers to identify circulating myeloid cell subsets. Based on CD14 and CD16 phenotypes, monocyte subsets described as classical (CD14+CD16-), intermediate (CD14+CD16+), and nonclassical (CD14loCD16+) were identified. Levels of surface APRIL expression were measured by flow cytometry and median fluorescence intensity was used for comparisons. Levels of soluble APRIL in the plasma were determined by ELISA. Disease activity was measured by the Disease Activity Score in 28 joints. RESULTS: In patients with RA, total myeloid cells showed expression of surface APRIL that correlated with disease activity and with plasma APRIL levels observed in these patients. In healthy donors, classical monocytes were composed of > 80% of circulating monocytes. However, in patients with RA, the intermediate and nonclassical subsets were elevated and made up the majority of circulating monocytes. In contrast to healthy donors, where high levels of surface APRIL were only observed in nonclassical monocytes, patients with RA showed high levels of surface APRIL expression by all circulating monocyte subsets. CONCLUSION: Surface APRIL is elevated in circulating myeloid cells in patients with RA where it is highly correlated with disease activity. Patients with RA also showed skewing of monocytes toward subsets associated with secretion of tumor necrosis factor-α and/or interleukin 1ß.


Asunto(s)
Artritis Reumatoide/metabolismo , Células Mieloides/metabolismo , Miembro 13 de la Superfamilia de Ligandos de Factores de Necrosis Tumoral/metabolismo , Anciano , Artritis Reumatoide/diagnóstico , Femenino , Humanos , Interleucina-1beta/metabolismo , Leucocitos Mononucleares/metabolismo , Masculino , Persona de Mediana Edad , Monocitos/metabolismo , Índice de Severidad de la Enfermedad , Factor de Necrosis Tumoral alfa/metabolismo
15.
Chem Res Toxicol ; 28(2): 256-61, 2015 Feb 16.
Artículo en Inglés | MEDLINE | ID: mdl-25622494

RESUMEN

Sulfur mustard binds to reactive cysteine residues, forming a stable sulfur-hydroxyethylthioethyl [SHETE]adduct that can be used as a long-term biomarker of sulfur mustard exposure in humans. The digestion of sulfur mustard-exposed blood samples with proteinase K following total protein precipitation with acetone produces the tripeptide biomarker [S-HETE]-Cys-Pro-Phe. The adducted tripeptide is purified by solid phase extraction, separated by ultra high pressure liquid chromatography, and detected by isotope dilution tandem mass spectrometry. This approach was thoroughly validated and characterized in our laboratory. The average interday relative standard deviation was ≤ 9.49%, and the range of accuracy was between 96.1 and 109% over a concentration range of 3.00 to 250. ng/mL with a calculated limit of detection of1.74 ng/mL. A full 96-well plate can be processed and analyzed in 8 h, which is 5 times faster than our previous 96-well plate method and only requires 50 µL of serum, plasma, or whole blood. Extensive ruggedness and stability studies and matrix comparisons were conducted to create a robust, easily transferrable method. As a result, a simple and high-throughput method has been developed and validated for the quantitation of sulfur mustard blood protein adducts in low volume blood specimens which should be readily adaptable for quantifying human exposures to other alkylating agents.


Asunto(s)
Proteínas Sanguíneas/química , Gas Mostaza/análisis , Gas Mostaza/química , Espectrometría de Masas en Tándem/métodos , Cromatografía Líquida de Alta Presión , Voluntarios Sanos , Humanos , Técnicas de Dilución del Indicador , Isótopos , Estructura Molecular
16.
Bogotá; s.n; 2014. 1-40 p. tab, ilus.
Tesis en Español | MOSAICO - Salud integrativa, LILACS | ID: biblio-1007729

RESUMEN

Los productos a base de plantas medicinales se pueden considerar como medicamentos, debido a sus efectos terapéuticos y reacciones adversas. La evidencia científica muestra actividad antiséptica, anti fúngica, reepitelizante, cicatrizante y anti-inflamatoria, que hace que las plantas posean efectos terapéuticos dependiendo su dosificación frente a diversas patologías oculares como conjuntivitis, ojo seco, dacriocistitis, blefaritis, demódex o enfermedades degenerativas. Por esta razón se presenta mediante una herramienta interactiva, las propiedades antimicrobianas en patología ocular, de las plantas medicinales. Objetivo: Caracterizar la evidencia científica del uso de plantas medicinales a nivel ocular a través de un recurso innovador e interactivo. Materiales y métodos: A través de las bases de datos Medline, Science Direct, Pubmed, ProQuest, E-libro, Embase. Se recopiló información asociada al uso de plantas medicinales a nivel general y ocular mediante los términos de búsqueda "antimicrobial activity medicinal plants ocular"," antimicrobial activity medicinal plants eye journal"," review antibacterial effects medicinal plants ocular infection multicentre research"," plantas medicinales a nivel ocular", "etnobotánica", "fitoterapia ocular" "medicinal plants and conjunctivitis", "medicinal plants and blepharitis", "medicinal plants and pterigion" La información se filtró de acuerdo con las fuentes de búsqueda, de libros, webs y artículos de los últimos quince años. Se recopiló información de la historia de la medicina natural, historia de las plantas medicinales, evidencia científica general y ocular de cada una de las plantas, seguidamente se filtró la información de las plantas medicinales seleccionando las más usadas a nivel ocular, con el fin de que el recurso interactivo tenga evidencia clínica actualizada. Finalmente se reprodujo por medio de un recurso interactivo el cual contiene información necesaria para dar al lector un conocimiento amplio de las características de las plantas medicinales y de sus bondades curativas para el tratamiento de las enfermedades oculares del segmento anterior. Conclusiones: Las plantas medicinales con más evidencia científica son Aloe Vera, Eufrasia, Melaleuca, Sándalo y Ajo, quienes muestran eficacia en bacterias como Estafilococos Aureus, Enterococos Escherichia coli, Aspergillus flavus; en diversas patologías oculares como conjuntivitis, blefaritis, ojo seco, dacriocistitis y queratitis. (AU)


Asunto(s)
Plantas Medicinales , Infecciones del Ojo , Colombia , Medicina Tradicional
17.
J Cataract Refract Surg ; 32(7): 1115-8, 2006 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-16857497

RESUMEN

PURPOSE: To assess the frequency and risk factors for intraoperative anesthesia consultation when performing cataract surgery monitored by registered nurses. SETTING: Iowa City Veterans Affairs Medical Center, Iowa City, Iowa, USA. METHODS: This retrospective review was of 270 cataract surgeries performed under local anesthesia from April 1, 2002, to April 1, 2003. RESULTS: The American Society of Anesthesiologists (ASA) classification of each patient was determined: 1 patient was classified as ASA 1. One hundred fifty patients were classified as ASA 2. One hundred nineteen patients were classified as ASA 3. The anesthesiology department was consulted 24 times. Nineteen consultations involved patients who were ASA 3, and 5 consultations involved patients who were ASA 2 (P<.001). In most cases (23 of 24), the anesthesia service provided a consultation (eg, increase oxygen flow rate, clarification of electrocardiogram, start intravenous line, equipment repair) and left the nurses to continue to monitor the patient. In only 1 case (ASA 3), the anesthesia service converted the case to monitored anesthesia care and relieved the nurse to monitor the patient. CONCLUSIONS: In this study, monitoring of routine cataract surgery by registered nurses was associated with a low rate of intraoperative anesthesia consultation. Most consultations resulted in little intervention. The ASA classification appears predictive of the need for intraoperative anesthesia consultation.


Asunto(s)
Anestesiología , Necesidades y Demandas de Servicios de Salud/estadística & datos numéricos , Cuidados Intraoperatorios/estadística & datos numéricos , Monitoreo Intraoperatorio/métodos , Enfermeras Anestesistas/estadística & datos numéricos , Facoemulsificación , Derivación y Consulta/estadística & datos numéricos , Anestesia Local/métodos , Anestésicos Locales/administración & dosificación , Humanos , Concesión de Licencias , Estudios Retrospectivos , Factores de Riesgo , Recursos Humanos
18.
Col. med. estado Táchira ; 11(2): 26-35, jul.-sept. 2002. graf
Artículo en Español | LILACS | ID: lil-417355

RESUMEN

Estudio prospectivo para determinar la relación entre el peso del niño al nacer y el estado nutricional materno. Se estudiaron 28 mujeres sanas con sus neonatos. En ellas se evaluó: índice de masa corporal, ganancia de peso, circunferencia de brazo izquierdo, hemoglobina, hematocrito; en ellos: peso, talla e índice ponderal según edad gestacional. Las madres resultaron 85,71 por ciento eutroficas, 7,14 por ciento obesas y 7,14 por ciento en riesgo de desnutrición. Los neonatos fueron 75 por ciento eutróficos y 14,28 por ciento delgados. El 60,71 por ciento de los niños eutróficos proviene de madres eutróficas. Se concluyó que la mayoría de las madres evaluadas son eutróficas, la minoría con alto riesgo de tener niños de bajo peso


Asunto(s)
Humanos , Femenino , Embarazo , Recién Nacido , Peso al Nacer , Desnutrición Proteico-Calórica/diagnóstico , Nutrición Materna , Salud Pública , Medicina , Ciencias de la Nutrición
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