Asunto(s)
Inhibidor p16 de la Quinasa Dependiente de Ciclina , Tumor de Células de la Granulosa , Neoplasias Ováricas , Proteína p53 Supresora de Tumor , Humanos , Femenino , Tumor de Células de la Granulosa/genética , Tumor de Células de la Granulosa/patología , Neoplasias Ováricas/genética , Neoplasias Ováricas/patología , Neoplasias Ováricas/cirugía , Proteína p53 Supresora de Tumor/genética , Proteína p53 Supresora de Tumor/metabolismo , Adolescente , Inhibidor p16 de la Quinasa Dependiente de Ciclina/genética , Inhibidor p16 de la Quinasa Dependiente de Ciclina/metabolismo , Inhibidor p15 de las Quinasas Dependientes de la Ciclina/genética , Mutación , Recurrencia Local de NeoplasiaRESUMEN
Objective: To investigate the clinicopathologic and molecular characteristics of fumarate hydratase (FH) deficient uterine leiomyoma. Methods: Eighty cases of FH deficient uterine leiomyoma were diagnosed from April 2018 to September 2022 in Department of Pathology, Peking University Third Hospital. Sanger sequencing of FH gene exons (exon 1-10) were performed on tumor tissues and matched non-tumor tissues/peripheral blood for all cases. FH immunohistochemistry were performed in 74 cases; S-(2-succino)-cysteine (2SC) were also detected by immunohistochemistry in five cases. Results: Patients' age ranged from 18 to 54 (36.0±7.5) years, with more than 60% exhibiting clinical symptoms of multiple and large leiomyomas (the median diameter was 70 mm). More than four histologic features, including staghorn vasculature, alveolar-pattern edema, bizarre nuclei, oval nuclei arranged in chains, prominent eosinophilic nucleoli with perinucleolar haloes and eosinophilic intracytoplasmic globules were observed in 98.5% (67/68) patients. The immunohistochemical sensitivity of FH and 2SC were 97.3% and 100%, respectively. Based on the Sanger sequencing results, the cases were divided into germline variant group (31 cases), somatic variant group (29 cases) and no variant group (20 cases). Sixty-nine percent (20/29) of the patients with FH germline variation had clear family history. Conclusions: Clinical features, histological morphology, FH and 2SC immunohistochemistry and Sanger sequencing have their own significance and limitations in differential diagnosis of FH deficient uterine leiomyoma. In clinical practice, the above information should be fully integrated and studied for accurate pathologic diagnosis and selection of patients with FH germline variation.
Asunto(s)
Carcinoma de Células Renales , Leiomioma , Leiomiomatosis , Neoplasias Uterinas , Femenino , Humanos , Adolescente , Adulto Joven , Adulto , Persona de Mediana Edad , Fumarato Hidratasa/genética , Neoplasias Uterinas/patología , Leiomioma/genética , Leiomioma/patología , Mutación de Línea Germinal , Diagnóstico Diferencial , Leiomiomatosis/diagnóstico , Leiomiomatosis/genética , Leiomiomatosis/patología , Carcinoma de Células Renales/diagnósticoRESUMEN
All of 20 patients with phosphorus burn, admitted from 1987 to 1991, have been cured by intravenous drip of calcium gluconate combined with other therapies. The wounds were washed with tap water, followed by eschar formation in 12 days. The clinical data showed that phosphorus poisoning did not occur if calcium was given very early postburn. Calcium also could abolish or ameliorate clinical manifestations of existing phosphorus poisoning, accelerate the elimination of phosphorus and recovery of damaged organs.
Asunto(s)
Quemaduras Químicas/tratamiento farmacológico , Gluconato de Calcio/uso terapéutico , Fósforo/envenenamiento , Adulto , Humanos , Masculino , Intoxicación/tratamiento farmacológicoRESUMEN
20 patients with phosphor burn (TBSA 2%-75%) were cured by i.v. drop of calcium gluconate combined with other therapies including eschar conservation. Our experimental data showed that dogs with burn by spreading 85% phosphoric acid and napalm locally increased the level of plasma phosphorus and pathological damages to the heart, lung, kidney and etc were similar to those previously reported phosphorus burns. Intravenous drop of calcium gluconate after phosphate burn reduced the level of plasma phosphorus to normal rapidly and lessened the visceral damages. We consider that i.v. drop of calcium gluconate can accelerate the elimination of phosphorus, and prevent phosphorus poisoning after phosphorus burns.