[Clinical effect of simultaneous implantation and maxillary sinus floor elevation with or without bone graft].

PURPOSE: To investigate the difference of osteogenic effect and stability of maxillary sinus implants 6 months after maxillary sinus elevation with or without bone grafting. METHODS: A total of 150 patients with maxillary sinus floor lift and simultaneous implantation in Lishui People's Hospital from December 2019 to December 2021 were divided into group A (internal maxillary sinus lift + bone grafting) and group B (internal lift without bone grafting). Preoperative and postoperative CBCT data and implant stability of all patients were measured and analyzed to compare whether there were differences in clinical efficacy between the two groups. SPSS 25.0 software package was used for data analysis. RESULTS: A total of 199 implants were implanted, the implant retention rate was 97.6% in group A and 95.7% in group B one year after surgery, with no significant difference between the two groups(P＞0.05). There was no significant difference in residual bone height(RBH) and gray scale value (HU) between the two groups before and 6 months after operation (P＞0.05). There was no significant difference in ISQ value between the two groups during operation and 6 months after operation(P＞0.05). CONCLUSIONS: In cases with the remaining alveolar bone height of 3～8 mm and the planned lifting height of 3～4 mm, maxillary sinus floor lifting procedure achieved good clinical results in both groups with or without bone grafting, which indicated that bone grafting had little effect on retention rate and stability of the implants.

[Effect of rat allogeneic BMSCs-Bio-Oss-bFGF compound on tooth extraction healing: a micro-CT study].

PRUPOSE: To investigate the effect of a compound of BMSCs-Bio-Oss-bFGF on microstructure of extraction sockets in rats. METHODS: Bone mesenchymal stem cells (BMSCs) were isolated from bone marrow of 3-week SD rats by adherent method. Maxillary posterior teeth of 36 6-week SD rats were extracted and materials were implanted into sockets according to grouping. The rats were divided into 4 groups: compound group with implanting BMSCs-Bio-Oss-bFGF compound, powder group with implanting Bio-Oss, BMSCs group with implanting BMSCs, and control group without implanting any materials. The sockets were scanned by micro-CT 4 weeks, 12 weeks and 24 weeks after implantation. Two-way ANOVA was used to assess whether there was significant difference between groups with GraphPad Prism 6.0 software package. RESULTS: There was no significant difference among groups in bone mineral density (BMD), trabecular separation(Tb.Sp), trabecular thickness(Tb.Th), degree of anisotropy(DA), and trabecular number(Tb.N) 4 weeks after implantation. By 12 weeks, BMD of compound group was significantly greater than those of BMSCs group, powder group and control group (P＜0.05), and significantly greater than those of powder group and control group at 24 weeks (P＜0.05). Tb.Th of compound group was significantly greater than that of BMSCs group at 12 and 24 weeks(P＜0.05). DA had no significant difference among groups at 4, 12, and 24 weeks (P＞0.05). Tb.Sp of compound group was significantly smaller than those of powder group, BMSCs group and control group at 24 weeks(P＜0.05). Tb.N of compound group was significantly greater than those of BMSCs group and control group(P＜0.05). CONCLUSIONS: The compound of rat allogeneic BMSCs-Bio-Oss-bFGF improves socket healing.

[Expression of fibroblast growth factor receptor like 1 protein in oral squamous cell carcinoma and its influence on tumor cell proliferation and migration].

OBJECTIVE: This study aims to investigate the expression of fibroblast growth factor receptor like 1 (FGFRL1) in oral squamous cell carcinoma (OSCC) and reveals its association with tumor cell proliferation and migration. METHODS: Western blot was performed to detect the expression of FGFRL1 protein in OSCC tissues, adjacent normal tissues, OSCC cell lines and normal epithelial cells. After knocking down of FGFRL1 in HN4 cells, CCK-8 and Ki67 assays were performed to detect cell proliferation, wounding healing assay and transwell were performed to detect cell-migration. Western blot was used to detect the expression of protein related to epithelial-mesenchymal transition (EMT). RESULTS: The expression of FGFRL1 in OSCC tissues was higher than that in adjacent nontumor tissues, respectively (t=2.820, P=0.047 8). Moreover, the expression of FGFRL1 in OSCC cells was higher than that in HOK cells. Quantitative real-time polymerase chain reaction (qRT-PCR) showed that FGFRL1 expression of FGFRL1 RNA in HOK cells was lower than that in OSCC cells. HN4 cells transfected with FGFRL1 siRNA were included in the experimental group, whereas HN4 cells treated with NC siRNA were included in the control group. CCK-8 experiment showed no significant difference between the experimental and control groups with regard to proliferation ability at 48 h (P=0.478 1) and 72 h (P=0.334 2). Migration experiment showed that the wound healing areas in the experimental group after 12 h (P=0.022 8), 24 h (P=0.005 1), and 36 h (P=0.009 5）were smaller than that in the control group. Transwell invasion assay showed that the number of invaded cells in the experimental group after 16 h (P=0.008 7) and 24 h (P=0.008 6) were lower than that in the control group. Knocking-down FGFRL1 up-regulated the expression of E-cadherin and down-regulated the expression of N-cadherin and Vimentin in HN4 cells. CONCLUSIONS: FGFRL1 expression in the OSCC tissues was significantly higher than that in the adjacent nontumor tissues. FGFRL1 expression in the OSCC cells was significantly higher than that in the HOK cells, and FGFRL1 had no effect on cell proliferation but promoted tumor cell migration and EMT.

[Cone-beam CT evaluation of volume and surface area changes of impacted upper central incisors before and after orthodontic treatment].

PURPOSE: The purpose of this study was to quantitatively evaluate root resorption by cone-beam computer tomography (CBCT) after orthodontic treatment of impacted upper central incisors. METHODS: Ten patients (12 impacted incisors and 8 contralateral corresponding teeth) were included in this study. With self-designed modified nance arch and straight wire arch, the impacted incisors were treated by combined orthodontics and surgery approach. CBCT images of all patients were taken before and immediately after the treatment. Mimics software was used to build three-dimensional images. The upper central incisors were isolated. Volumes of teeth and superficial area were calculated. The difference between pre- and post- treatment was statistically evaluated with paired samples t test using SPSS13.0 software package. RESULTS: Significant difference in volume and superficial area was found between pre- and post- treatment (P<0.05). The average whole dental volume increase was (9.67±10.86) mm3, and the superficial area increase was (28.97±14.36)mm2. Statistical significance was achieved among 12 impacted incisors. The average whole dental volume increase of the control group was（12.38±10.22）mm3, and the superficial area increase was（30.64±16.74）mm2. There was no significant difference between the impacted and the control group in dental volume and superficial area before and after treatment (P>0.05). CONCLUSIONS: The treatment by combined orthodontics and surgery approach could promote tooth development for impacted incisors without causing root absorption, which can be safely performed.

[Efficacy of bFGF atomization inhalation on postoperative sore throat following oral and maxillofacial surgery under general anesthesia].

PURPOSE: To observe the effect of recombinant human basic fibroblast growth factor (bFGF) atomization inhalation on postoperative sore throat following oral and maxillofacial operation under general anethesia. METHODS: Forty patients in whom oral and maxillofacial operation trachea was removed under general anesthesia were randomly divided into treatment and control groups; the treatment group received bFGF 35000IU + normal saline 20 mL, compression inhalation, day 1, every 20min, continued for 3 d; the control group was given normal saline 5 mL + dexamethasone 5 mg + gentamicin 80000 U + chymotrypsin 4000 U, compression inhalation, twice a day, every 20 min, continued for 3 d. Occurrence of postoperative sore throat 12 h after operation was recorded and visual analog scale (VAS) of sore throat (swallowing) 12,24,48,72 h after operation was measured. SPSS l4.0 software package was used for statistical analysis. RESULTS: The incidence of sore throat in the treatment group was significantly lower than that in the control group (P<0.01) 12 h after operation; sore throat (swallowing) VAS 12,24,48 and 72 h after operation in the treatment group were significantly lower than that in the control group (P<0.05). CONCLUSIONS: bFGF atomization inhalation can reduce the incidence of sore throat and sore throat level in oral and maxillofacial surgery after endotracheal intubation.

Guidelines for the treatment of head and neck venous malformations.

Venous malformation is one of the most common benign vascular lesions, with approximately 40% of cases appearing in the head and neck. They can affect a patient's appearance and functionality and even cause life-threatening bleeding or respiratory tract obstruction. The current methods of treatment include surgery, laser therapy, sclerotherapy, or a combined. The treatment of small and superficial venous malformations is relatively simple and effective; however, the treatment of deep and extensive lesions involving multiple anatomical sites remains a challenge for the physicians. For complex cases, the outcomes achieved with one single treatment approach are poor; therefore, individualized treatment modalities must be formulated based on the patient's condition and the techniques available. Comprehensive multidisciplinary treatments have been adapted to achieve the most effective results. In this paper, based on the national and international literature, we formulated the treatment guidelines for head and neck venous malformations to standardize clinical practice. The guideline will be renewed and updated in a timely manner to reflect cutting-edge knowledge and to provide the best treatment modalities for patients.

[Xeroderma pigmentosum with repeated facial skin carcinogenesis: report of one case].

Xeroderma pigmentosum is a relatively rare pigment atrophic autosomal recessive genetic disease, mainly due to defect of DNA excision repair capacity, resulting in ineffective removal of UV-induced pyrimidine dimers, and finally cell damage. This paper reported a case of xeroderma pigmentosum with recurrent episodes of oral and maxillofacial carcinogenesis, and discussed the etiology, pathology, clinical characteristics, classification, diagnosis and treatment methods of Xeroderma pigmentosum.

[Treatment of infantile hemangiomas with long pulsed 1064nm laser and oral corticosteroids].

PURPOSE: To evaluate the clinical therapeutic efficacy on infantile hemangiomas using long pulsed 1064nm laser and oral corticosteroid. METHODS: One hundred and two infantile hemangiomas in the proliferating phase state were treated with long pulsed 1064nm laser, long pulsed 1064nm laser and oral corticosteroid .Color Doppler was used to detect the depth of tumor. Laser treatment was conducted for 1 to 3 sessions, and oral corticosteroid was one course for 12 weeks. The results were analyzed with SAS6.12 software package for Ridit test. RESULTS: Total response treated with long pulsed 1064nm laser, long pulsed 1064nm laser and oral corticosteroid were achieved in 12 cases(24.00%) and 14 cases(26.92%)respectively, good response were achieved in 18 cases(36.00%) and 27 cases(51.92%). There was significant difference between the two groups(P<0.05). Total response in patients with superficial lesions treated with long pulsed 1064nm laser, long pulsed 1064nm laser and oral corticosteroid were achieved in 8 cases(34.78%) and 7 cases(33.33%) respectively, while good response were achieved in 10 cases(43.48%) and 8 cases(38.10%).There was no significant difference between two groups(P>0.05).Total response in patients with deep lesions treated with long pulsed 1064nm laser, long pulsed 1064nm laser and oral corticosteroid were achieved in 4 cases(14.81%) and 7 cases(22.58%) respectively, good response were achieved in 8 cases(29.63%) and 19 cases(61.29%).There was significant difference between the two groups(P<0.05). CONCLUSION: Combined therapy with long pulsed 1064nm laser and oral corticosteroid is effective for infantile deep-seated hemangiomas in the proliferating stage.

Mushroom-shaped teratoma of the soft palate in a neonate: case report.

Teratomas of the head and neck are uncommon congenital lesions, which are rarely seen in the soft palate. We here reported a male neonate with a soft palate teratoma associated with soft palate cleft. The most common presenting symptom of oral teratoma is feeding problems. The tumour looks like a mushroom, and was successfully treated with surgery. Feeding returned to normal by removing the mass, and the patient is under follow up for selective operation of the cleft palate. This case is unique because teratoma in a mushroom shape was not reported before.

[Peripheral ameloblastoma in mandibular gingiva: Report of one case].

The ameloblastoma mainly occurs in the mandible, rarely seen in the soft tissues. This paper reported 1 case of mandibular gingival peripheral ameloblastoma, and discussed its treatment based on literature review.

[Clinical analysis of 193 patients with thickening and nodular port wine stains].

PURPOSE: The purpose of this study is to investigate the relation between age, gender and thickening and nodules in port wine stains(PWS) patients. METHODS: The gender and age of 193 cases with port wine stains were compared between those with flat, those with thickening, those with thickening and nodules, in 0-19, 20-year age group. SAS6012 software package was used for Fisher's exact test and Kruskal-Wallis test. RESULTS: There were 165 cases with flat (85.49%), 10 cases with thickening (5.18%), 18 cases with thickening and nodules (9.33%). Kruskal-Wallis test demonstrated significant difference between 2 age groups(P<0.01).There was an increase incidence of thickening, and thickening and nodule formation in 0-19, 20-year age group(P<0.01), but there was no gender difference between thickening and nodules.The data were analyzed by SAS6.12 Fisher's exact test and Kruskal-Wallis test. CONCLUSION: The incidence of thickening and nodule formation in PWS increase with age.

[Clinical analysis of neurofibromatosis in oral and maxillofacial regions treated with staged operations and laser therapy].

PURPOSE: To evaluate the clinical effect of neurofibromatosis in the oral and maxillofacial region treated with staged operations and laser therapy. METHODS: Eight cases with neurofibromatosis were treated with staged operations, laser therapy was used for hemostasis during operation, cafe-au-lait was treated with Q-switched laser. RESULTS: The average amount of bleeding was 100-400 ml, no hemorrhagic shock occurred intraoperatively. All patients were of good facial appearance, cafe-au-lait was improved during short-term follow-up. CONCLUSION: Staged operations and laser therapy is a valuable treatment for neurofibromatosis in the oral and maxillofacial region.

[Lip and cheek reconstruction with composite radial forearm-palmaris longus tendon flap].

PURPOSE: To investigate the value of functional reconstruction of the lip and cheek with composite radial forearm-palmaris longus tendon flap. METHODS: Composite radial forearm-palmaris longus tendon flap was used in 5 cases, the flap was folded for repair of oral mucosa and external skin, palmaris longus tendon and the remaining orbicularis muscles were sutured so that oral contour and function could be achieved. All the patients were followed up for at least 20 months. RESULTS: All 5 composite flaps survived well postoperatively. 6 months after reconstruction, the patients had good oral and facial contour; 10-12 months after surgery, the dynamic function of mouth opening and closing as well as mastication was recovered to normal. CONCLUSION: Composite radial forearm-palmaris longus tendon flap is reliable and thus recommended for functional reconstruction of the lip and cheek.

[Thyroid hormone level change in patients undergoing oral and maxillofacial surgery].

PURPOSE: To study the changes of thyroid hormone level in patients undergoing oral and maxillofacial surgery before and after operation, in order to provide some evidences for postoperative treatment and recovery. METHODS: 60 cases with oral and maxillofacial operations under general or local anesthesia were assayed by radioimmunoassay for hormone levels changes of T3 ,T4, r T3 and TSH in serum before operation and on the first, forth and seventh day after operation. SPSS10.0 software package was used for one way ANOVA. RESULTS: Compared to preoperative levels, T3, T4 and r T3 (P<0.01) as well as TSH (P<0.05) in patients receiving operations under general anesthesia on the first, forth and seventh day postoperatively decreased significantly, while the changes of thyroid hormone level in patients receiving operations under local anesthesia before and after operation were not significantly different. CONCLUSION: Thyroid hormone level change in patients who underwent oral and maxillofacial surgery may be related to the type of operation and anesthesia.