Mesenchymal Stromal Cell (MSC) Functional Analysis-Macrophage Activation and Polarization Assays.

Stem cell-based therapies have evolved to become a key component of regenerative medicine approaches to human pathologies. Exogenous stem cell transplantation takes advantage of the potential of stem cells to self-renew, differentiate, home to sites of injury, and sufficiently evade the immune system to remain viable for the release of anti-inflammatory cytokines, chemokines, and growth factors. Common to many pathologies is the exacerbation of inflammation at the injury site by proinflammatory macrophages. An increasing body of evidence has demonstrated that mesenchymal stromal cells (MSCs) can influence the immunophenotype and function of myeloid lineage cells to promote therapeutic effects. Understanding the degree to which MSCs can modulate the phenotype of macrophages within an inflammatory environment is of interest when considering strategies for targeted cell therapies. There is a critical need for potency assays to elucidate these intercellular interactions in vitro and provide insight into potential mechanisms of action attributable to the immunomodulatory and polarizing capacities of MSCs, as well as other cells with immunomodulatory potential. However, the complexity of the responses, in terms of cell phenotypes and characteristics, timing of these interactions, and the degree to which cell contact is involved, have made the study of these interactions challenging. To provide a research tool to study the direct interactions between MSCs and macrophages, we developed a potency assay that directly co-cultures MSCs with naïve macrophages under proinflammatory conditions. Using this assay, we demonstrated changes in the macrophage secretome and phenotype, which can be used to evaluate the abilities of the cell samples to influence the cell microenvironment. These results suggest the immunomodulatory effects of MSCs on macrophages while revealing key cytokines and phenotypic changes that may inform their efficacy as potential cellular therapies. Key features • The protocol uses monocytes differentiated into naïve macrophages, which are loosely adherent, have a relatively homogeneous genetic background, and resemble peripheral blood mononuclear cells-derived macrophages. • The protocol requires a plate reader and a flow cytometer with the ability to detect six fluorophores. • The protocol provides a quantitative measurement of co-culture conditions by the addition of a fixed number of freshly thawed or culture-rescued MSCs to macrophages. • This protocol uses assessment of the secretome and cell harvest to independently verify the nature of the interactions between macrophages and MSCs.

The application of electrosprayed minocycline-loaded PLGA microparticles for the treatment of glioblastoma.

The survival of patients with glioblastoma multiforme (GBM), the most common and invasive form of malignant brain tumors, remains poor despite advances in current treatment methods including surgery, radiotherapy, and chemotherapy. Minocycline is a semi-synthetic tetracycline derivative that has been widely used as an antibiotic and more recently, it has been utilized as an antiangiogenic factor to inhibit tumorigenesis. The objective of this study was to investigate the utilization of electrospraying process to fabricate minocycline-loaded poly(lactic-co-glycolic acid) (PLGA) microparticles with high drug loading and loading efficiency and to evaluate their ability to induce cell toxicity in human glioblastoma (i.e., U87-MG) cells. The results from this study demonstrated that solvent mixture of dicholoromethane (DCM) and methanol is the optimal solvent combination for minocycline and larger amount of methanol (i.e., 70:30) resulted in a higher drug loading. All three solvent ratios of DCM:methanol tested produced microparticles that were both spherical and smooth, all in the micron size range. The electrosprayed microparticles were able to elicit a cytotoxic response in U87-MG glioblastoma cells at a lower concentration of drug compared to the free drug. This work provides proof of concept to the hypothesis that electrosprayed minocycline-loaded PLGA microparticles can be a promising agent for the treatment of GBM and could have potential application for cancer therapies.

Longitudinal two-dimensional gas chromatography mass spectrometry as a non-destructive at-line monitoring tool during cell manufacturing identifies volatile features correlative to cell product quality.

BACKGROUND AIMS: Cell therapies have emerged as a potentially transformative therapeutic modality in many chronic and incurable diseases. However, inherent donor and patient variabilities, complex manufacturing processes, lack of well-defined critical quality attributes and unavailability of in-line or at-line process or product analytical technologies result in significant variance in cell product quality and clinical trial outcomes. New approaches for overcoming these challenges are needed to realize the potential of cell therapies. METHODS: Here the authors developed an untargeted two-dimensional gas chromatography mass spectrometry (GC×GC-MS)-based method for non-destructive longitudinal at-line monitoring of cells during manufacturing to discover correlative volatile biomarkers of cell proliferation and end product potency. RESULTS: Specifically, using mesenchymal stromal cell cultures as a model, the authors demonstrated that GC×GC-MS of the culture medium headspace can effectively discriminate between media types and tissue sources. Headspace GC×GC-MS identified specific volatile compounds that showed a strong correlation with cell expansion and product functionality quantified by indoleamine-2,3-dioxygenase and T-cell proliferation/suppression assays. Additionally, the authors discovered increases in specific volatile metabolites when cells were treated with inflammatory stimulation. CONCLUSIONS: This work establishes GC×GC-MS as an at-line process analytical technology for cell manufacturing that could improve culture robustness and may be used to non-destructively monitor culture state and correlate with end product function.

Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccine.

Despite success in vaccinating populations against SARS-CoV-2, concerns about immunity duration, continued efficacy against emerging variants, protection from infection and transmission, and worldwide vaccine availability remain. Molecular adjuvants targeting pattern recognition receptors (PRRs) on antigen-presenting cells (APCs) could improve and broaden the efficacy and durability of vaccine responses. Native SARS-CoV-2 infection stimulates various PRRs, including toll-like receptors (TLRs) and retinoic acid-inducible gene I (RIG-I)-like receptors. We hypothesized that targeting PRRs using molecular adjuvants on nanoparticles (NPs) along with a stabilized spike protein antigen could stimulate broad and efficient immune responses. Adjuvants targeting TLR4 (MPLA), TLR7/8 (R848), TLR9 (CpG), and RIG-I (PUUC) delivered on degradable polymer NPs were combined with the S1 subunit of spike protein and assessed in vitro with isogeneic mixed lymphocyte reactions (isoMLRs). For in vivo studies, the adjuvant-NPs were combined with stabilized spike protein or spike-conjugated NPs and assessed using a two-dose intranasal or intramuscular vaccination model in mice. Combination adjuvant-NPs simultaneously targeting TLR and RIG-I receptors (MPLA+PUUC, CpG+PUUC, and R848+PUUC) differentially induced T cell proliferation and increased proinflammatory cytokine secretion by APCs in vitro. When delivered intranasally, MPLA+PUUC NPs enhanced CD4+CD44+ activated memory T cell responses against spike protein in the lungs while MPLA NPs increased anti-spike IgA in the bronchoalveolar (BAL) fluid and IgG in the blood. Following intramuscular delivery, PUUC NPs induced strong humoral immune responses, characterized by increases in anti-spike IgG in the blood and germinal center B cell populations (GL7+ and BCL6+ B cells) in the draining lymph nodes (dLNs). MPLA+PUUC NPs further boosted spike protein-neutralizing antibody titers and T follicular helper cell populations in the dLNs. These results suggest that protein subunit vaccines with particle-delivered molecular adjuvants targeting TLR4 and RIG-I could lead to robust and unique route-specific adaptive immune responses against SARS-CoV-2.

Theranostic nanocarriers combining high drug loading and magnetic particle imaging.

We report preparation of theranostic nanocarriers loaded with up to 50 wt% of the anticancer drug doxorubicin that contain magnetic nanoparticles which enable Magnetic Particle Imaging (MPI), an emerging technology for quantitative and unambiguous imaging of the nanocarriers. The nanocarriers, coated with poly(ethylene glycol)-block-poly(lactic acid) (PEG4.9kD-b-PLA6kD) block copolymer for colloidal stability, are composed of a hydrophobic core of precipitated hydrolysable doxorubicin prodrug (proDox) and magnetic nanoparticles. Transmission electron microscopy (TEM) shows evidence of precipitated proDox for nanocarriers with high drug loading of up to 50 wt%. MPI measurements show that the nanocarriers can be quantitatively imaged. The nanocarriers are internalized by MDA-MB-231 cells and their IC50 value via metabolic assay is 1.1 µM, compared to 0.21 µM for free doxorubicin. The release rate from the nanocarriers was dependent on environmental pH. These nanocarriers with high drug loading and quantitative imaging are promising candidates for future applications.

Cáncer de laringe y sinunasal en Cali, Colombia 1962 ­ 2015 / Sinonasal and laryngeal carcinoma in Cali, Colombia 1962 ­ 2015

Introducción: El cáncer es una enfermedad de alta incidencia, con múltiples factores etiológicos y diferentes características evolutivas. Objetivo: Describir la supervivencia y la tendencia de las tasas de incidencia y mortalidad por cáncer de laringe y senos paranasales en Cali (Colombia) durante el periodo 1962 a 2015. Diseño: Estudio observacional descriptivo. Métodos: La información de incidencia, durante el periodo 1962 a 2012, se obtuvo en el RPCC y la mortalidad entre 1984-2015 de la SSPM. El comportamiento de la tendencia se evaluó con el porcentaje de cambio anual (APC) y la supervivencia relativa se estimó con el método de Ederer II. La estadificación se realizó con la AJCC. Resultados: Durante 1962 a 2012, se diagnosticaron 1623 casos nuevos de cáncer, 85.2% (1383 casos) fueron de laringe y 14.8% (240 casos) sinunasal 76% fueron hombres y el CCE fue el tipo histológico más frecuente 84.1% y 59.6% en laringe y sinunasal respectivamente. Se observó una disminución significativa del riesgo de cáncer de laringe en ambos sexos, siendo mayor la disminución en hombres (APC=-1,1*(ICD95%: -1,6; -0,7)). La disminución en la tasa de incidencia de cáncer de laringe CEC fue mayor en hombres (APC=-1,1*(ICD95%: -1,6; -0,6)) durante 1962-2012. Las tasas de mortalidad disminuyeron significativamente (APC=- 2,5*(ICD95%:-3,6 -1,5)). La supervivencia relativa a 5 años durante el periodo 2008- 2012, 43,5%; 93 pacientes se estadificaron con supervivencia: T3-T4 (41,5%) y T1- T2 (55,0%). Conclusiones: El riesgo del cáncer de laringe y sinunasal escamocelular asociado al tabaquismo disminuyó de manera significativa en hombres y mujeres de Cali durante los últimos 53 años.

Introduction: Cancer is disease with high incidence, multiple etiological factors and different characteristics. Objective: To describe the survival and trend of the incidence and mortality rates for cancer of the larynx and paranasal sinuses in Cali (Colombia) during the period 1962 to 2015. Design: Descriptive observational study. Methods: Incidence information, during the period 1962 to 2012, was obtained in the RPCC and mortality between 1984-2015 of the SSPM. The behavior of the trend was evaluated with the percentage of annual change (APC) and the relative survival was estimated with the method of Ederer II. The arrangement was made with the AJCC. Results: During 1962 to 2012, 1623 new cases of cancer were diagnosed, 85.2% (1383 cases) were laryngeal and 14.8% (240 cases) sinunasal 76% were men and the CCE was the most frequent histological type 84.1% and 59.6% in larynx and sinunasal respectively. A significant decrease in the risk of laryngeal cancer was observed in both sexes, with a greater decrease in men (APC = -1.1 * (ICD95%: -1.6, -0.7)). The decrease in the incidence rate of laryngeal cancer CEC was higher in men (APC = -1.1 * (ICD95%: -1.6, -0.6)) during 1962-2012. Mortality rates decreased significantly (APC = -2.5 * (ICD95%: - 3.6 -1.5)). The 5-year relative survival rate during the period 2008-2012 was 43.5%; 93 patients were stratified with survival: T3-T4 (41.5%) and T1-T2 (55.0%). Conclusions: The risk of laryngeal and squamous cell sinonasal cancer associated to smoking decreased significantly in men and women of Cali during the last 53 years.

Local 3D matrix confinement determines division axis through cell shape.

How the division axis is determined in mammalian cells embedded in three-dimensional (3D) matrices remains elusive, despite that many types of cells divide in 3D environments. Cells on two-dimensional (2D) substrates typically round up completely to divide. Here, we show that in 3D collagen matrices, mammalian cells such as HT1080 human fibrosarcoma and MDA-MB-231 breast cancer cells exhibit division modes distinct from their Counterparts on 2D substrates, with a markedly higher fraction of cells remaining highly elongated through mitosis in 3D matrices. The long axis of elongated mitotic cells accurately predicts the division axis, independently of matrix density and cell-matrix interactions. This 3D-specific elongated division mode is determined by the local confinement produced by the matrix and the ability of cells to protrude and locally remodel the matrix via ß1 integrin. Elongated division is readily recapitulated using collagen-coated microfabricated channels. Cells depleted of ß1 integrin still divide in the elongated mode in microchannels, suggesting that 3D confinement is sufficient to induce the elongated cell-division phenotype.

Hispanic health in the USA: a scoping review of the literature.

Hispanics are the largest minority group in the USA. They contribute to the economy, cultural diversity, and health of the nation. Assessing their health status and health needs is key to inform health policy formulation and program implementation. To this end, we conducted a scoping review of the literature and national statistics on Hispanic health in the USA using a modified social-ecological framework that includes social determinants of health, health disparities, risk factors, and health services, as they shape the leading causes of morbidity and mortality. These social, environmental, and biological forces have modified the epidemiologic profile of Hispanics in the USA, with cancer being the leading cause of mortality, followed by cardiovascular diseases and unintentional injuries. Implementation of the Affordable Care Act has resulted in improved access to health services for Hispanics, but challenges remain due to limited cultural sensitivity, health literacy, and a shortage of Hispanic health care providers. Acculturation barriers and underinsured or uninsured status remain as major obstacles to health care access. Advantageous health outcomes from the "Hispanic Mortality Paradox" and the "Latina Birth Outcomes Paradox" persist, but health gains may be offset in the future by increasing rates of obesity and diabetes. Recommendations focus on the adoption of the Health in All Policies framework, expanding access to health care, developing cultural sensitivity in the health care workforce, and generating and disseminating research findings on Hispanic health.

Correlación de variables antropométricas como predictor de salud, en una población de niños y adolescentes con síndrome de Down de Temuco, Chile / Correlating anthropometric variables as health predictors in a group of children and adolescents suffering Down's syndrome in Temuco, Chile

Antecedentes. En los últimos años se ha evidenciado la importancia de investigar sobre la obesidad, incluyendo a grupos de sujetos que presentan necesidades educativas especiales permanentes, como es el caso del Síndrome de Down. Objetivo. Determinar la correlación entre el Índice de Masa Corporal (IMC) con la circunferencia de cintura (CCi) y el IMC con la circunferencia de cadera (CCa) de los niños y adolescentes con síndrome de Down. Materiales y métodos. Se realizó un estudio de corte transversal, donde se evaluaron 42 niños y adolescentes, 23 hombres (54,7%) y 19 mujeres (35,3%), entre 3 y 16 años de edad, alumnos de escuelas especiales de Temuco, Chile. Para obtener el IMC y la CCa se utilizó el método descrito por la OMS. Resultados. Se evidencia un alto grado de correlación entre IMC y CCi (0,776) y entre IMC y CCa (0,771). Además el 61,9% del total presentan sobrepeso u obesidad. El 52,1% de los hombres y el 73,6% de las mujeres presentan obesidad o sobrepeso. Discusión. A partir de los resultados, es recomendable la utilización de la circunferencia de cintura y cadera, además del IMC para la detección temprana de problemas asociados a la obesidad. Además de focalizar el trabajo a realizar con esta población especial, generando estrategias que involucren a distintos profesionales del ámbito de la salud y del ejercicio físico, permitiendo abordar sistémicamente desde los primeros años la prevención de la obesidad y las patologías asociadas.

Background. The importance of investigating obesity has grown during recent years, especially regarding groups of people involving long-term special education needs, such as Down's syndrome. Objective. Determining the correlation between body mass index (BMI) and waist circumference and BMI and hip circumference in children and teenagers suffering Down's syndrome. Materials and methods. This cross-sectional study involved evaluating 42 children and adolescents aged 3 to 16 years old; 23 of them were male (54.7%) and 19 female (35.3%) and all were studying in specialised schools in Temuco, Chile. The method described by the WHO was used for obtaining BMI, waist and hip circumference measurements. Results. A high correlation was seen for both BMI and waist circumference (0.776) and BMI and hip circumference (0.771). It was also seen that 61.9% of the population studied were overweight or obese; 52.1% of the males and 73.6% of the females were either obese or overweight. Discussion. It is thus recommended that waist and hip circumference and BMI should be used for the early detection of obesity-related problems. Efforts should be specifically focused on this special population of people, leading to strategies involving healthcare workers in different areas as well as physical exercise. This would lead to the systematic prevention of obesity and associated pathologies from an early age.

Caraterísticas psicológicas en 15 niños con estreñimientos crónico funcional del Hospital Universitario del Valle de Cali, Colombia / Psychological characteristics in 15 children with chronic functional constipation of the Hospital Universitario del Valle from Cali, Colombia

Introducción: El manejo integral de los niños con Estreñimiento Crónico Funcional (ECF), incluye nutrición, medicamentos, y educación, con la intervención de varios profesionales de la salud, que ofrezcan un tratamiento integral, inter, trans y multidisciplinario. Los aspectos médicos y psicológicos, si bien están disociados en cuanto a su perspectiva de intervención, se unen en cuanto al interés referente al niño visto de manera integral. Objetivo: Reportar las características psicológicas de 15 niños del Hospital Universitario del Valle (HUV) de Cali, Colombia, con ECF. Metodología: Estudio descriptivo observacional de corte transversal en niños entre los 2 y 12 años de edad, 9 masculinos, que asistieron al Servicio de Gastroenterología Pediátrica del HUV de Cali, Colombia durante el segundo semestre de 2010, a quienes se les realizó una entrevista semiestructurada. Resultados: Diez madres presentaron inconvenientes durante su embarazo (no deseado, intento de aborto, tratamientos para fertilidad, amenaza de aborto, antecedentes de abortos, ansiedad, parto complicado); 4 niños fueron manipulados con enemas o estimulación; 3 fueron castigados físicamente durante el proceso de control de esfínteres; 10 fueron entrenados para el control de esfínteres antes de los 2 años; 4 presentaron enuresis/terrores nocturnos; 7 tenían sus padres separados, y 8 padres maltrataban física o psicológicamente a sus hijos. Conclusión: Los niños con ECF suscitan para la familia y profesionales de la salud, estados de ansiedad, confusión y frustración. Se identificaron dificultades físicas emocionales durante el embarazo, dificultad o temor a fallar en el papel materno, problemas conyugales, y educación precoz en el control de esfínteres; que si bien son normales durante la crianza de los niños, recibieron un manejo inadecuado.

Introduction: Integrated management of children with Chronic Functional Constipation (CFC), includingnutrition, drugs, and education, with the involvement of various health professionals who provide comprehensive inter, tranas and multidisciplinary treatment. Medical and psychological aspects, although they are differenciated in terms of intervention perspective, join in interest concerning the child seen holistically. Objective: To report the psychological characteristics of 15 children with CFC at the Hospital Universitario del Valle (HUV) from Cali, Colombia. Methodology: A descriptive crosssectional observational study in children with 2 and 12 years old; 9 males; attending the Pediatric Gastroenterology Service, during the second half of 2010, who underwent a semistructured interview. Results: Ten mothers had problems during her pregnancy (unwanted, attempeted abortion, fertility treatments, threatened abortion, history of abrotions, anxiety, complicated delivery); 4 children were manipulated with enemas or stimulation; 3 were physically punished during the potty training process; 10 were trained for toliet control before 2 years; 4 had enuresis/night terrors; 7 parents had separed, and 8 parents physically or psychologically abused their children. Conclusion: Children with CFC raise the family and health professionals, anxiety, confusión and frustation. Emotional physical difficulties were indentified during pregnancy, difficulty or fear of failure in the maternal role, marital problems, and early education in controlling sphincters, that are normal butfor the rearing of children received inadequate mangement.

Caracterización psicológica de las familias de niños con enfermedad por reflujo gastroesofágico / Psychological characteristics of families from children with gastroesophageal reflux disease

Objetivo: Materiales y Métodos: La observación de los niños con ERGE y sus familias nos permitió iniciar la comprensión inherente a la enfermedad y todos los temores, y expectativas. Por lo tanto, el conflicto emocional debido a la pérdida de control del mundo externo, sus regularidades y predicciones es evidente, y esto conduce al aumento de ansiedad cuando se enfrentan a la enfermedad física del niño. Durante las entrevistas semiestructuradas llevadas a cabo con las familias de los niños con ERGE, se han encontrado características recurrentes que aluden a una serie de manifestaciones propias de la persona cuando es amenazada por factores en los que no tiene el control. En ese sentido, se observan una serie de defensas que son convenientes en ese momento para soportar el dolor y la incapacidad que la enfermedad del niño representa. Entre las defensas más notables están la negación, la disociación, la intelectualización, la proyección y la regresión, manifestaciones cuyo componente principal es la pérdida de control y la incapacidad de proporcionar al niño la protección que él requiere. Por lo tanto, el conocimiento que el pediatra tiene sobre las capacidades psicológicas del niño y la familia, así como el reconocimiento de la amenaza emocional que la enfermedad implica a los padres, se traducirá en un apoyo adicional a fin de proteger la salud y hacer el proceso un poco más tolerable.

Objective: To describe the psychological characteristics in families of children withGERD. Materials and Methods.Thus, the emotional conflict due to the loss of control of the external world, its regularities and predictions is evident, and this leads to the anxiety increase when facing the physical illness of the child. During the semistructured interviews carried out with the families of the children with GERD, recurrent characteristics that allude to a series of manifestations proper of the person when is threaten by factors on which does not have control have been found. In that sense, a series of defenses that are fine at that moment for bearing the pain and the incapacity that the child's illness represents are observed. Among the most remarkable defenses are: negation, dissociation, intellectualization, projection and regression, manifestations which main component is the loss of control and the inability to provide the child the protection s/he requires. Therefore, the knowledge that the pediatrician has about the psychological capabilities of both the child and the family, as well as the recognition of the emotional threat that the illness implies to the parents will result in an extra support in order to protect the health and make the process a little more tolerable.

[Attention to speed and guide traffic signs with eye movements]. / Atención a señales de velocidad y de orientación mediante movimientos oculares.

The goal of this research is to describe the visual search patterns for diverse traffic signs. Twelve drivers of both genders and different driving experience levels took part in real driving research with an instrumented car provided with an eye-tracking system. Looking at signs has a weak relation with speed reduction in cases where actual driving speed was higher. Nevertheless, among the people who looked at the sign, the percentage of those who reduce the speed below the limit is greater than of those who do not look at the sign. Guide traffic signs, particularly those mounted over the road, are more frequently glanced at than speed limit signs, with a glance duration of more than one second, in sequences of more than two consecutive fixations. Implications for driving and the possibilities and limitations of eye movement analysis for traffic sign research are discussed.

Relación entre hipertensión arterial sistémica y síndrome de apnea-hipopnea obstructiva del sueño y sus factores de resgo asociados, en población hipertensa de un centro médico: Cali (Colombia)2008 / Relation between systemic hypertension and apnea-hypopnea syndrome and its associated risk factors in hypertensive population from a medical center: Cali (Colombia) 2008

Introducción: La relación causa efecto entre la hipertensión arterial sistémica y un mayor riesgo para desarrollar síndrome de apnea-hipopnea obstructiva del sueño (SAHOS) es aún incierta. El desconocimiento de la influencia del SAHOS sobre la hipertensión arterial ha llevado al excesivo diagnóstico de hipertensión arterial idiopática, desmotivando la búsqueda de la real causa subyacente, que en la mayoría de los casos puede deberse a trastornos del sueño. Materiales y métodos: En el presente estudio se incluyen pacientes hipertensos quienes consultaron en el centro médico ONIRIS, especializado en sueño, y se evaluó en ellos variables como índice de masa corporal, índice cintura cadera, circunferencia del cuello, tabaquismo y alcohol, para determinar cuáles de ellos desarrollaron SAHOS. Resultados: De 309 pacientes estudiados, el 67,4 por ciento fue positivo para SAHOS, confirmando algún grado de asociación entre ésta y la hipertensión arterial. Además, variables incluidas como factores de riesgo arrojaron resultados muy similares a los obtenidos en otros estudios que han demostrado dicha asociación. Conclusiones: Tiene predominio por el sexo masculino, se presenta generalmente en mayores de 50 años y obesos; dichos factores ponen en un riesgo elevado al paciente de desarrollar eventos vasculares que disminuyen la calidad de vida e incluso llevarlo a la muerte.

Dynamic surface activity by folding and unfolding an amphiphilic alpha-helix.

We describe a rationally designed peptide with tunable surface activity, where the dynamics of surface activity are an outcome of helical folding. Our rationally designed model peptide is surface-active only as an alpha-helix. We apply circular dichroism to show that the folded population can be controlled with changes in electrolyte concentration, and we apply pendant bubble tensiometry to explore dynamic surfactant activity. This study shows a peptide that responds to environmental stimuli with dynamic folding and surface activity. Extending this concept to selective binding peptides will lead to new tools, where dynamic surface activity is coupled to targeted binding.