RESUMEN
Sertoli-Leydig cell tumours account for less than 0.2% of ovarian malignancies. We describe the case of a Sertoli-Leydig cell tumour in a 13-year-old girl who presented with symptoms of hyperandrogenism. We give an overview over current literature and discuss options of therapy of rare Sertoli-Leydig cell tumours.
Asunto(s)
Neoplasias Ováricas/diagnóstico , Tumor de Células de Sertoli-Leydig/diagnóstico , Adolescente , Femenino , Hirsutismo/diagnóstico , Hirsutismo/etiología , Humanos , Hiperandrogenismo/diagnóstico , Hiperandrogenismo/etiología , Neoplasias Ováricas/complicaciones , Neoplasias Ováricas/patología , Tumor de Células de Sertoli-Leydig/complicaciones , Tumor de Células de Sertoli-Leydig/patologíaRESUMEN
AF6 and its rat homologue afadin are multidomain proteins localized at cell junctions and involved in intercellular adhesion. AF6 interacts via its PDZ domain with nectin-1 at epithelial adherens junctions. Nectin-1 serves as a mediator of cell-to-cell spread for Herpes simplex virus 1 (HSV-1). We analyzed the role of AF6 protein in the viral spread and nectin-1 clustering at cell-cell contacts by knockdown of AF6 in epithelial cells. AF6 knockdown reduced efficiency of HSV-1 spreading, however, the clustering of nectin-1 at cell-cell contacts was not affected. Thus, AF6 protein is important for spreading of HSV-1 in epithelial cells, independently of nectin clustering, possibly by stabilization of the E-cadherin-dependent cell adhesion.
Asunto(s)
Herpesvirus Humano 1/fisiología , Cinesinas/metabolismo , Miosinas/metabolismo , Internalización del Virus , Animales , Moléculas de Adhesión Celular/metabolismo , Comunicación Celular , Línea Celular , Chlorocebus aethiops , Humanos , Cinesinas/genética , Miosinas/genética , NectinasRESUMEN
We report here that the interferon-induced protein of 10 kDa (IP-10 or CXCL10) elicits strong anti-tumor and anti-metastatic responses in mice when administered by plasmid DNA. Intratumoral but not intramuscular IP-10 DNA inoculation resulted in reduced tumor formation of malignant melanoma (B16F10) and Lewis lung carcinoma (LL/2) in C57BL/6 mice. In addition, plasmid DNA-encoding IP-10 substantially reduced the establishment of metastases when injected systemically by the intramuscular route. In contrast to the primary tumor model, the anti-metastatic effect of DNA-encoding IP-10 was primarily mediated by NK cells. Compared to DNA-encoding interleukin-12 (IL-12), therapy with DNA-encoding IP-10 exhibits lower efficacy against primary melanoma tumors but equivalent efficacy against primary Lewis lung tumors and against B16F10 lung metastasis formation. Co-administration of DNA-encoding IP-10 and IL-12 enhanced the anti-tumor activity of IL-12 in the lung metastasis model but had little effect in the local treatment of established subcutaneous tumors. Interestingly, treatment of nude mice lacking T lymphocytes with DNA-encoding IP-10 or IL-12 still resulted in a pronounced reduction of tumor growth or metastasis formation.